以莺哥凤梨(Vriesea carinata)为外类群,利用Clustal X1.81和MEGA3.1软件对31种铁兰属植物的基因序列进行比对和分析,并构建分子系统树。结果表明:31种铁兰属植物供试材料的trnL-F序列长度在806～835 bp之间,变异位点22个,信息位点14个,平均遗传距离0.007,且在301～322 bp和826～830 bp处分别出现了插入和polyT序列。利用ML法建立的系统树显示,31个铁兰属植物分为3组。序列分析结果与形态学结果基本一致,表明trnL-F序列可用于铁兰属植物的亲缘关系分析,并可作为某些特定种的鉴定依据。
Abstract
In this paper, Catopsis subulata was selected as the outgroup, and trnL-F genes of 31 Tillandsia species were analyzed and compared with each other to construct the phylogenetic trees by use of Clusta1 X1.81 and MEGA3.1 softwares. The results showed that the length of trnL-F genes in 31 Tillandsia plants were in the range of 806 bp to 835 bp, and there were 22 variable sites and 14 informative sites among them. On the other hand, the average genetic distance of 31 trnL-F gene sequences was 0.007, and at the points of 301-322 bp,there were 21 deoxyribonucleotides inserted in trnL-F gene sequences from T.fasciculata and T.tenuifolia,and a polyT sequence appeared at 826-830 bp.The phylogenetic trees built by ML method illustrated that 31 species could be clustered together into three groups. The clustered results were consistent with the morphologic identification. This indicated that trnL-F gene could be used for the Tillandsia relationship analysis and as the basis for the identification of the specific species.
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参考文献
[1] 宿静.中国引种的铁兰属植物系统分类及微形态研究[D].南京:南京林业大学,2010.Su J.Phylogenetic relationship analysis and morphology of Tillandsia plants[D].Nanjing:Nanjing Forestry University,2010.
[2] Mez C. Bromeliaceae[C]// Endler A. Das Pflanzenreich.W. Leipzig: Engelmann, 1934-1935.
[3] Smith L B,Downs R J. Pitcairnioideae(Bromeliaceae)[C]// Flora Neotropica Monograph,1974.
[4] Smith L B,Downs R J. Tillandsioideae(Bromeliaceae)[C]// Flora Neotropica Monograph,1977.
[5] Hebert P D,Cywinska A,Ball S L, et al. Biological identifications through DNA barcodes[J]. Proceedings of the Royal Society of London Series B:Biological Sciences, 2003,270:313-321.
[6] 张蓉,殷亚方,徐魁梧,等.基于DNA的木材树种识别进展[J].南京林业大学学报:自然科学版,2014,38(1):151-156.Zhang R, Yin Y F, Xu Q W, et al Progress of wood identification based on DNA method[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2014, 38(1):151-156.
[7] Richardson J E,Fay M F,Cronk Q B,et al. A phylogenetic analysis of Rhamnaceae using rbcL and trnL-F plastid DNA sequences[J].Amer J Bot, 2000,87(9):1309-1324.
[8] Bayer R J, Puttock C F, Kelchner S A. Phylogeny of south African Gnaphalieae(Asteraceae)based on two non-coding chloroplast sequences[J].Amer J Bot, 2000,87:259-272.
[9] Tablert P,Gielly L,Pautou G,et al. Universal primers for amplification of three non-coding regions of chloroplast DNA[J].Plant Mol Bio, 1991,17(5):1105-1109.
[10] 赵卫国,张志芳,潘一乐,等.桑树trnL-F基因间隔区序列的特点及分析[J].蚕业科学, 2002,28(2):83-86.Zhao W G,Zhang Z F,Pan Y L,et al. Studied on the cpDNA trnL-F intergenic spacer sequence in the genus mulberry[J].Science of sericulture,2002,28(2):83-86.
[11] Thompson J D,Gibson T J,Plewniak F,et al. The CLUSTAL X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools[J].Nucleic Acids Research, 1997,25(24):4876-4882.
[12] 宿静,李玉萍,汤庚国. 34种铁兰属植物亲缘关系的SRAP分析[J].南京林业大学学报:自然科学版,2013,37(5):153-157.Su J,Li Y P,Tang G G. Genetic relationship of 34 Tillandsia plants using SRAP marker[J]. Journal of Nanjing Forestry University: Natural Sciences Edition,2013,37(5):153-157.
[13] Small R L,Rybum J A,Cronn R C,et al. The tortoise and the hare:choosing between non-coding plastome and nuclear Adh sequences for phylogeny reconstruction in a recently diverged plant group[J].Amer J Bot, 1998,85:1301-1315.
[14] 杨路存,陈桂琛,周国英.基于不同DNA序列对四数璋牙菜系统位置分析[J].西北植物学报,2010,30(9):1773-1779.Yang L C, Chen G C, Zhou G Y. Systematic position of Swertia tetraptera Maxim. inferred from ITS, rpl16 and trnl-trnf DNA sequences[J].Acta Botanica Boreali-Occidentalia Sinica, 2010,30(9):1773-1779.
[15] Wang F Y, Gong X, Hu C M,et al. Phylogeography of an alpine species Primula secundiflora inferred from the chloroplast DNA sequence variation[J].Journal of Systematics and Evolution, 2008,46(1):13-22.
[16] Liu Y, Yan H F, Cao T,et al. Evaluation of 10 plant barcodes in Brayophyta(Mosses)[J].Journal of Systematics and Evolution, 2010,48(1):36-46.
基金
收稿日期:2013-03-15 修回日期:2014-01-07
基金项目:江苏省镇江市科技计划项目(NY2007044)
第一作者:宿静,讲师。E-mail: sujing-418@163.com。
引文格式:宿静. 基于trnL-F基因序列的铁兰属植物亲缘关系分析[J]. 南京林业大学学报:自然科学版,2014,38(3):175-178.
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