低温胁迫下梁山慈竹再生植株叶绿素荧光特性和耐寒转录因子的表达

陈容; 张丽; 曹颖; 卢学琴; 胡尚连; 段宁

doi:10.3969/j.issn.1000-2006.2014.04.008

陈容,张丽,曹颖,卢学琴,胡尚连,段宁

南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (04) : 39-44.

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南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (04) : 39-44. DOI: 10.3969/j.issn.1000-2006.2014.04.008

研究论文

低温胁迫下梁山慈竹再生植株叶绿素荧光特性和耐寒转录因子的表达

陈容, 张丽, 曹颖, 卢学琴, 胡尚连^*, 段宁

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Chlorophyll fluorescence parameters and expression of cold-related transcription factors in regenerated plants of Dendrocalamus farinosus under cold stress

CHEN Rong, ZHANG Li, CAO Ying, LU Xueqin, HU Shanglian^*, DUAN Ning

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摘要

以生长期一致的梁山慈竹实生苗(SS)和梁山慈竹种子成熟胚离体诱导愈伤组织获得的再生植株(No.101-1c、No.42-1-B、No.90-3)为材料,分析不同低温胁迫下其叶绿素荧光参数和耐寒相关转录因子表达量的变化,研究低温胁迫对梁山慈竹再生植株耐寒性的影响。结果表明:SS可耐受0 ℃低温处理,No.90-3仅能耐受4 ℃低温处理,而No.101-1c和No.42-1-B可以耐受-10 ℃低温处理,表明不同再生植株对低温胁迫的耐受能力不同,且与实生苗也有明显差异。低温胁迫条件下, SS、No.101-1c与No.90-3的F_v/F_m、Y(Ⅱ)和qP值均下降; No.42-1-B的Y(Ⅱ)值先上升后下降,其F_v/F_m、NPQ和qP则随温度降低而下降。实生苗的WRKY与CBF1基因的表达量降低,MYB基因的表达量上升; 再生植株No.90-3和No.42-1-B的MYB、WRKY和CBF1基因的表达量均随温度降低而升高; No.101-1c的WRKY和CBF1基因表达量上升,而MYB表达量下降。相关性分析结果表明:实生苗中WRKY和CBF1的表达量与Y(Ⅱ)的值呈显著的正相关关系; No.101-1c中MYB的表达量与Y(Ⅱ)的值呈显著的正相关关系,而WRKY和CBF1的表达量与NPQ的值呈显著的负相关关系; No.42-1-B中WRKY的表达量与qP的值呈显著的负相关关系。

Abstract

In order to explore the cold-resistance of the regenerated plants from Dendrocalamus farinosus, the seedlings of D. farinosus(SS)and three regenerated plants(No.101-1c, No.42-1-B and No.90-3)induced from D. farinosus mature embryo in vitro were exposed to cold stress in this study. Their chlorophyll fluorescence parameters and expression level of cold-related transcription factors under cold stress were investigated. It aimed to the results showed that SS could be resistant to 0 ℃; No.90-3 could be resistant to 4 ℃,while No.101-1c and No.42-1-B could be resistant to-10 ℃. It indicated that the cold-resistance of three regenerated plants was different and had obvious difference with SS. Under cold stress, F_v/F_m, Y(Ⅱ)and qP of SS, No.101-1c and No.90-3 decreased with the temperature decreasing, while Y(Ⅱ) of No.42-1-B went up and then descended, but the F_v/F_m, NPQ and qP reduced. The expression level of WRKY and CBF1 from SS decreased, while the expression level of MYB increased. In No.90-3 and No.42-1-B, the increase of expression level of MYB, WRKY and CBF1 was observed, and the transcription accumulation of WRKY and CBF1 from No.101-1c increased, but the expression level of MYB reduced under the cold stress. The results of correlation analysis showed that the expression of WRKY and CBF1 from SS had a significant positive relationship with Y(Ⅱ); the relationship between MYB and Y(Ⅱ)from No.101-1c was positive significantly, while there was a significant negative relationship among NPQ, WRKY and CBF1. In addition, WRKY showed a significant negative relationship to qP of No.42-1-B.

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陈容,张丽,曹颖,卢学琴,胡尚连,段宁. 低温胁迫下梁山慈竹再生植株叶绿素荧光特性和耐寒转录因子的表达[J]. 南京林业大学学报（自然科学版）. 2014, 38(04): 39-44 https://doi.org/10.3969/j.issn.1000-2006.2014.04.008

CHEN Rong, ZHANG Li, CAO Ying, LU Xueqin, HU Shanglian, DUAN Ning. Chlorophyll fluorescence parameters and expression of cold-related transcription factors in regenerated plants of Dendrocalamus farinosus under cold stress[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2014, 38(04): 39-44 https://doi.org/10.3969/j.issn.1000-2006.2014.04.008

中图分类号： S718.46

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基金

收稿日期:2013-07-07 修回日期:2013-11-05
基金项目:四川省应用基础研究基金项目(2013JY0182)
第一作者:陈容,硕士生。*通信作者:胡尚连,教授,博士。E-mail: hushanglian@126.com。
引文格式:陈容, 张丽, 曹颖,等. 低温胁迫下梁山慈竹再生植株叶绿素荧光特性和耐寒转录因子的表达[J]. 南京林业大学学报:自然科学版,2014,38(4):39-44.