以新疆枸杞和枸杞1年生实生苗为试验材料,通过测定NaHCO3胁迫下两种枸杞苗木干质量、叶片MDA、H2O2含量、脯氨酸含量、Na+和K+含量、电解质外渗率及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸氧化酶(APX)和谷胱甘肽还原酶(GR)等抗氧化酶活性的变化,研究NaHCO3胁迫下两种枸杞渗透调节及抗氧化生理响应机制。结果表明:轻度胁迫促进两种枸杞的生长,随胁迫强度的增加两种枸杞生长受到抑制。两种枸杞叶片Na+和脯氨酸含量随胁迫强度的增加逐渐升高,且新疆枸杞明显高于枸杞。与枸杞相比,新疆枸杞受到的氧化伤害较轻,表现为较低的叶片H2O2和MDA含量及电解质外渗率。随胁迫强度的增加,两种枸杞的SOD活性逐渐增加,CAT活性逐渐降低。两种枸杞的POD活性变化情况与SOD相似,但重度胁迫下枸杞POD活性显著低于对照。两种枸杞APX和GR活性在较低强度胁迫下升高,而在较高强度胁迫下降低。不同的是,重度胁迫下新疆枸杞APX和GR活性虽有所下降,但仍显著高于对照,但重度胁迫下枸杞APX和GR活性却已明显低于对照。研究表明,新疆枸杞的抗NaHCO3能力强于枸杞。分析认为,NaHCO3胁迫下新疆枸杞较好的表现可能是由于其更好的渗透调节能力和更为有效的抗氧化防御体系。
Abstract
In order to explore the tolerant mechanism of two species of Lycium to NaHCO3 stress, the responses of osmotic regulation and antioxidant systems to NaHCO3 stress in Lycium dasystemum and Lycium chinense were investigated by determining the dry weight, the electrolyte leakage, the contents of malondialdehyde(MDA), H2O2, proline, Na+ and K+ as well as the activities of antioxidant enzymes like superoxide dismutase(SOD), catalase(CAT), peroxidase(POD), ascorbate peroxidase(APX)and glutathione reductase(GR). The results showed that growth of two Lycium species were improved under lower stress intensity as compared to that of control. The contents of Na+ and proline in leaves increased with the stress intensity increasing, while that of K+ decreased. The less oxidative damage of Lycium dasystemum was associated with lower MDA content, electrolyte leakage, and H2O2 concentration. With the increasing stress intensity, the activity of SOD increased, while that of CAT decreased gradually. The activities of APX and GR changed identically, which increased under lower NaHCO3 stress, and then began to decrease under severer NaHCO3 stress. These results presented that Lycium dasystemum exhibited a better protection mechanism against oxidative damage and it was more salt-tolerant than Lycium chinense possibly by maintaining better capability in osmotic regulation and a more effective antioxidant defense system.
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基金
收稿日期:2014-01-01 修回日期:2014-04-03
基金项目:吉林省科技发展计划项目(201201084); 吉林省四平市科技发展计划项目(四科合字2013048)
第一作者:刘强,讲师,博士。*通信作者:周晓梅,教授,博士。E-mail: 1402561846@qq.com。
引文格式:刘强,王占武,周晓梅. 两种枸杞对NaHCO3胁迫的抗性生理响应[J]. 南京林业大学学报:自然科学版,2014,38(6):165-169.
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