为了解松材线虫致病力及繁殖力的遗传变异规律,以松材线虫强毒虫株及弱毒虫株为亲本进行杂交试验, 并对杂交后代的繁殖力和致病力进行比较。结果表明:不同亲本间杂交成功率不同,同一对亲本的正交、反交成功率亦不同。大多数杂交后代群体在灰葡萄孢上和马尾松苗体内的繁殖力与亲本之间差异显著。杂交后代群体及强毒亲本接种马尾松5周后的致萎蔫率达90%～100%,而弱毒亲本接种的植株在10周后仅20%出现轻微的萎蔫症状。统计分析表明,松材线虫致病力与其在树体内的繁殖力间存在中等程度的正相关性,与灰葡萄孢上的繁殖力相关性较弱。聚类分析表明,不同毒力松材线虫杂交后代群体的致病力与繁殖力之间没有亲缘关系偏向,繁殖力与致病力不存在伴性遗传。
Abstract
In order to understand the genetic variation in pine wood nematode, virulent and avirulent isolates of Bursaphelenchus xylophilus were used as parental isolates to establish crossbred lines, and the virulence and reproductive ability of offsprings were compared with parental isolates. The results showed that hybrid rates were different among different parents, even one pair parents for reciprocal cross. A significant difference was observed in reproductive ability among parental isolates and some crossbred lines on Botrytis cinerea and in masson pine seedlings. The proportion of dead masson pine seedlings inoculating with virulent parent and crossbred lines ranged from 90%-100% after 5 weeks, while only 20% of the seedlings inoculating with avirulent parent showed slight wilting symptons 10 weeks later. Statistical analyses revealed that virulence was positively correlated with propagation rate inside the host pine seedlings in some extent, but was weakly correlated with propagation rate on B. cinerea. Cluster analysis showed that the biological parameters varied between the parents and offsprings, with no kinship bias, suggesting the absence of sex-linked inheritance in virulence and rates of propagation.
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基金
收稿日期:2014-11-03 修回日期:2014-12-08
基金项目:“十二五”国家科技支撑计划(2012BAD19B0703); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:朱丽华,副教授。*通信作者:叶建仁,教授。E-mail: jrye@njfu.com.cn。
引文格式:朱丽华,施学文,胡玉丹,等. 不同毒力松材线虫杂交后代的繁殖力和致病力的比较[J]. 南京林业大学学报:自然科学版,2015,39(1):1-5.
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