配位-有机溶剂法去除银杏叶初提物中银杏酚酸的研究

doi:10.3969/j.issn.1000-2006.2015.02.002

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南京林业大学学报（自然科学版） ›› 2015, Vol. 58 ›› Issue (02): 7-13.doi: 10.3969/j.issn.1000-2006.2015.02.002

配位-有机溶剂法去除银杏叶初提物中银杏酚酸的研究

杨世龙¹,黄春丽²,唐颖³,徐莉^2,3,4*,蒋国斌⁵, 曹福亮^4,5*,雷萌²,陈泳²,王燕芹²

1.南京林业大学化学工程学院,江苏南京 210037;
2.南京林业大学理学院,江苏南京 210037;
3.南京林业大学现代分析测试中心,江苏南京 210037;
4.南京林业大学南方现代林业协同创新中心,江苏南京 210037;
5.南京林业大学林学院,江苏南京 210037

出版日期:2015-03-31 发布日期:2015-03-31
基金资助:
收稿日期:2014-07-28 修回日期:2014-12-30
基金项目:国家林业公益性行业科研专项项目(201004015,20120460102); 江苏省第四期“333高层次人才培养工程”科研项目(BRA2012171); “十二五”国家科技支撑计划(2012BAD21B04); 南京林业大学优秀博士学位论文创新基金项目
第一作者:杨世龙,博士生。*通信作者:徐莉,教授。E-mail: xuliqby@163.com。曹福亮,教授。E-mail:caofl@njfu.edu.cn。
引文格式:杨世龙,黄春丽,唐颖,等. 配位-有机溶剂法去除银杏叶初提物中银杏酚酸的研究[J]. 南京林业大学学报:自然科学版,2015,39(2):7-13.

Study on removing ginkgolic acids from EGb by coordination-organic solvent method

YANG Shilong¹, HUANG Chunli², TANG Ying³, XU Li^2,3,4*, JIANG Guobin⁵, CAO Fuliang^4,5*, LEI Meng², CHEN Yong², WANG Yanqin²

1. College of Chemical Engineering, Nanjing Forestry University,Nanjing 210037, China;
2. College of Science, Nanjing Forestry University,Nanjing 210037, China;
3.Advanced Analysis and Testing Center, Nanjing Forestry University, Nanjing 210037, China;
4. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
5. College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Online:2015-03-31 Published:2015-03-31

摘要/Abstract

摘要： 银杏叶提取物(Extracts of Ginkgo biloba,EGb)具有诸多生物活性,但银杏酚酸的存在限制了EGb的应用。目前,去除EGb中银杏酚酸的方法主要有有机溶剂法和柱层析法,笔者在有机溶剂法的基础上提出配位-有机溶剂法,即在有机溶剂法最佳工艺条件的基础上引入金属离子配位以增加去除银杏酚酸的效果。结果表明:利用配位-有机溶剂法可以有效去除银杏酚酸,并有效减少有机溶剂洗涤次数,且不影响总银杏黄酮苷和总银杏内酯的含量; 同时,EGb中的部分黄酮与锌离子形成配合物,可以提高EGb的抗肝癌细胞(HepG2)的活性。

Abstract: Extracts of Ginkgo biloba (EGb)has many biological activities, however, the existence of ginkgolic acids in EGb limit the applications of EGb. Currently, organic solvent method and column chromatography method are the main ways to remove ginkgolic acids from EGb. As a new method, coordination-organic solvent method was put forward based on organic solvent method. In coordination-organic solvent method,metal ions were added to the mixed solution from the best technological conditions of organic solvent method. The results showed that the coordination-organic solvent method could not only effectively remove ginkgolic acids, but also reduce the extraction times without affecting the content of total flavonoid glycosides and total ginkgolides. Meanwhile, a small amount of flavonoids and zinc ions could form complexes, which could improve the EGb activity of anti-hepatoma cells(HepG2).

中图分类号:

杨世龙,黄春丽,唐颖,等. 配位-有机溶剂法去除银杏叶初提物中银杏酚酸的研究[J]. 南京林业大学学报（自然科学版）, 2015, 58(02): 7-13.

YANG Shilong, HUANG Chunli, TANG Ying, XU Li^2,3,4*, JIANG Guobin, CAO Fuliang^4,5*, LEI Meng, CHEN Yong, WANG Yanqin. Study on removing ginkgolic acids from EGb by coordination-organic solvent method[J].Journal of Nanjing Forestry University (Natural Science Edition), 2015, 58(02): 7-13.DOI: 10.3969/j.issn.1000-2006.2015.02.002.

参考文献

[1] 曹福亮,汪贵斌,郁万文. 银杏叶用林定向培育技术体系的集成[J]. 南京林业大学学报:自然科学版, 2014,38(6):146-152.Cao F L, Wang G B, Yu W W. Oriented cultivation techniques for leaf-harvest plantation of Ginkgo biloba L.[J]. Journal of Nanjing Forestry University: Natural Sciences Edition, 2014,38(6):146-152.
[2] Li X S, Zheng W Y, Lou S X, et al. Effect of ginkgo leaf extract on vascular endothelial function in patients with early stage diabetic nephropathy[J]. Journal of Chinese Integrative Medicine, 2009, 15(1): 26-29.
[3] Baliutyte Giedre, Baniene Rasa, Gendviliene Vida, et al. Influence of ethanol extract of Ginkgo biloba leaves on the isolated rat heart work and mitochondria functions[J]. Journal of Cardiovascular Pharmacology, 2012, 59(5): 450-457.
[4] Kehr J, Yoshitake S, Ijiri S, et al. Ginkgo biloba leaf extract(EGb761(R))and its specific acylated flavonol constituents increase dopamine and acetylcholine levels in the rat medial prefrontal cortex: possible implications for the cognitive enhancing properties of EGb761(R)[J]. International Psychogeriatrics, 2012, 24(1): 25-34.
[5] Cavusoglu Kueltigin, Yapar Kuersad, Yalcin Emine. Antioxidant potential of Ginkgo biloba leaf extract against uranium-induced genotoxicity and oxidative stress in albino mice[J]. Fresenius Environmental Bulletin, 2009, 18(9): 1551-1558.
[6] Yirmibesoglu E, Karahacioglu E, KilicD, et al. The protective effects of Ginkgo biloba extract(EGb-761)on radiation-induced dermatitis: an experimental study[J]. Clinical and Experimental Dermatology, 2012, 37(4): 387-394.
[7] Song X P, Chen Z W, Fang A N, et al. Experimental study on effect of total flavones of Ginkgo biloba on delaying cell senescence[J]. Journal of Chinese Medicinal Materials, 2009, 32(1): 100-102.
[8] Guan X N, Yan F L. Influence of Ginkgo biloba extract on beta-secretase in rat hippocampal neuronal cultures following chronic hypoxic and hypoglycemic conditions[J]. Neural Regeneration Research, 2008, 3(10): 1065-1069.
[9] Huang J C, Li J H, Liu T X. Effect of combined the rapy with hypha cordyceps and ginkgo leaf tablet on micro-inflammation in patients undergoing maintenance hemodialysis[J]. Chinese Journal of Integrated Traditional and Western Medicine, 2008, 28(6): 502-504.
[10] YinY Y, Min X F, Li W Z. Effects of ginkgolide on hemodynamics in myocardial ischemic dogs[J]. Journal of Chinese Medicinal Materials, 2010, 33(5): 759-762.
[11] Maria Stasiuk, AnnaJaromin, Arkadiusz Kozubek. The effect of merulinic acid on biomembranes[J]. Biochimicaet Biophysica Acta, 2004, 1667: 215-221.
[12] Karl Sch?tz. Quantification of allergenic urushiolsin extracts of Ginkgo biloba leaves, in simple one-step extracts and refined manufactured material(EGb761)[J]. Phytochemical Analysis, 2004(15): 1-8.
[13] 邵胜荣,王若谷,范秀林,等. 低酸银杏叶提取物的制备方法:中国,CN1318440C[P]. 2007-05-30.
[14] 倪力军,张立国. GBE中银杏酚酸的分离技术及其检测[J]. 中国中医药信息杂志, 2000, 7(6): 50-52.Ni L J, Zhang L G. Isolation technology and determination of ginkgolic acids from EGB[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2000, 7(6): 50-52.
[15] 曹治权. 中药药效的物质基础和作用机理研究新思路(一)[J]. 上海中医药大学学报, 2000, 14(1): 36-39.Cao Z Q. New thinking about study of pharmacodynamic material basis and functional mechanism in Chinese Materia Medica-study on the relation between morphology and biological activity of chemical species in Chinese Materia medica[J]. Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai, 2000, 14(1): 36-39.
[16] 曹治权. 中药微量元素研究的最新进展和展望[J]. 广东微量元素科学, 1997, 4(7): 9-16.Cao Z Q. Recent progress and prospect of the study on trace elements in traditional Chinese medicine[J]. Guangdong Trace Elements Science, 1997, 4(7): 9-16.
[17] Ravichandran R, Rajendran M, Devapiriam D. Antioxidant study of quercetin and their metal complex and determination of stability constant by spectrophotometry method [J]. Food Chemistry, 2014, 146: 472-478.
[18] Anife Ahmedova, Katarzyna Paradowska, Iwona Wawer. H-1, C-13 MAS NMR and DFT GIAO study of quercetin and its complex with Al(III)in solid state[J]. J Inorg Biochem, 2012, 110: 27-35.
[19] 谢伟玲,杨培慧,蔡继业. 锗(Ⅳ)-槲皮素配合物的制备、表征及其抗氧化活性测定[J]. 分析化学, 2010, 38(12):1809-1812.Xie W L, Yang P H, Cai J Y. Synthesis, characterization and antioxidation activity of Germanium(Ⅵ)-Quercetin complex[J]. Chinese Journal of Analytical Chemistry, 2010, 38(12):1809-1812.
[20] 刘燕,周轶平,罗敏,等. 稀土配合物抗肿瘤活性研究进展[J]. 中国稀土学报, 2014, 32(2): 143-155. Liu Y, Zhou Y P, Luo M, et al. Advances for antitumor activity of rare earth complexes[J]. Journal of the Chinese Society of Rare Earths. 2014, 32(2): 143-155.
[21] Gholamreza Dehghan, Zahra Khoshkam. Tin(II)-quercetin complex: Synthesis, spectral characterisation and antioxidant activity[J]. Food Chemistry, 2012, 131: 422-426.
[22] 刘平平,潘苏华. 银杏叶制剂中银杏酚酸研究进展[J]. 中国中药杂志, 2012, 37(3): 274-277.Liu P P,Pan S H. Advance in study of ginkgolic acid contained in Ginkgo biloba preparations[J]. China Journal of Chinese Materia Medica, 2012, 37(3): 274-277.
[23] 仰榴青,吴向阳,陈钧. 高效液相色谱法测定银杏外种皮中银杏酸的含量[J]. 分析化学, 2002, 30(8): 901-905.Yang L Q, Wu X Y, Chen J. Determination of ginkgolic acids in sarcotesta of Ginkgo biloba by high performance liquid chromatography[J]. Chinese Journal of Analytical Chemistry, 2002, 30(8): 901-905.
[24] 秦燕, 何新英, 庄慧娣, 等. HPLC测定银杏提取物中主要黄酮和萜内酯含量的优化方法[J]. 上海第二医科大学学报, 2003, 23(3): 234-236.Qin Y, He X Y, Zhuang H D, et al. An optilal method for determination of main flavonoids and terpenoids in Ginkgo biloba extract by HPLC[J]. Acta Universitatis Medicinalis Secondae Shanghai, 2003, 23(3): 234-236.
[25] 国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2010.
[26] Kelly L Wolfe, Liu H R. Cellular antioxidant activity(CAA)assay for assessing antioxidants, foods, and dietary supplements[J]. Journal of Agricultural and Food Chemistry, 2007, 55: 8896-8907.
[27] 李方,郑怀礼. 黄酮配合物抗自由基活性的亚甲基蓝光谱测定体系的研究[J]. 光谱学与光谱分析, 2006, 26(12):2294-2297.Li F, Zheng H L. Study on fenton-methylene blue spectral analysis method for determining the inhibitory effect of quercetin complexes on hydroxyl free radical[J]. Spectroscopy and Spectral Analysis, 2006, 26(12): 2294-2297.

配位-有机溶剂法去除银杏叶初提物中银杏酚酸的研究

Study on removing ginkgolic acids from EGb by coordination-organic solvent method

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