配位-有机溶剂法去除银杏叶初提物中银杏酚酸的研究

杨世龙; 黄春丽; 唐颖; 徐莉; 蒋国斌; 曹福亮; 雷萌; 陈泳; 王燕芹

doi:10.3969/j.issn.1000-2006.2015.02.002

杨世龙,黄春丽,唐颖,徐莉2,3,4*,蒋国斌,曹福亮4,5*,雷萌,陈泳,王燕芹

南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (02) : 7-13.

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南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (02) : 7-13. DOI: 10.3969/j.issn.1000-2006.2015.02.002

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配位-有机溶剂法去除银杏叶初提物中银杏酚酸的研究

杨世龙¹,黄春丽²,唐颖³,徐莉^2,3,4*,蒋国斌⁵, 曹福亮^4,5*,雷萌²,陈泳²,王燕芹²

作者信息 +

Study on removing ginkgolic acids from EGb by coordination-organic solvent method

YANG Shilong¹, HUANG Chunli², TANG Ying³, XU Li^2,3,4*, JIANG Guobin⁵, CAO Fuliang^4,5*, LEI Meng², CHEN Yong², WANG Yanqin²

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文章历史 +

摘要

银杏叶提取物(Extracts of Ginkgo biloba,EGb)具有诸多生物活性,但银杏酚酸的存在限制了EGb的应用。目前,去除EGb中银杏酚酸的方法主要有有机溶剂法和柱层析法,笔者在有机溶剂法的基础上提出配位-有机溶剂法,即在有机溶剂法最佳工艺条件的基础上引入金属离子配位以增加去除银杏酚酸的效果。结果表明:利用配位-有机溶剂法可以有效去除银杏酚酸,并有效减少有机溶剂洗涤次数,且不影响总银杏黄酮苷和总银杏内酯的含量; 同时,EGb中的部分黄酮与锌离子形成配合物,可以提高EGb的抗肝癌细胞(HepG2)的活性。

Abstract

Extracts of Ginkgo biloba (EGb)has many biological activities, however, the existence of ginkgolic acids in EGb limit the applications of EGb. Currently, organic solvent method and column chromatography method are the main ways to remove ginkgolic acids from EGb. As a new method, coordination-organic solvent method was put forward based on organic solvent method. In coordination-organic solvent method,metal ions were added to the mixed solution from the best technological conditions of organic solvent method. The results showed that the coordination-organic solvent method could not only effectively remove ginkgolic acids, but also reduce the extraction times without affecting the content of total flavonoid glycosides and total ginkgolides. Meanwhile, a small amount of flavonoids and zinc ions could form complexes, which could improve the EGb activity of anti-hepatoma cells(HepG2).

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杨世龙,黄春丽,唐颖,徐莉2,3,4*,蒋国斌,曹福亮4,5*,雷萌,陈泳,王燕芹. 配位-有机溶剂法去除银杏叶初提物中银杏酚酸的研究[J]. 南京林业大学学报（自然科学版）. 2015, 39(02): 7-13 https://doi.org/10.3969/j.issn.1000-2006.2015.02.002

YANG Shilong, HUANG Chunli, TANG Ying, XU Li^2,3,4*, JIANG Guobin, CAO Fuliang^4,5*, LEI Meng, CHEN Yong, WANG Yanqin. Study on removing ginkgolic acids from EGb by coordination-organic solvent method[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(02): 7-13 https://doi.org/10.3969/j.issn.1000-2006.2015.02.002

中图分类号： S792.95 TQ460

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基金

收稿日期:2014-07-28 修回日期:2014-12-30
基金项目:国家林业公益性行业科研专项项目(201004015,20120460102); 江苏省第四期“333高层次人才培养工程”科研项目(BRA2012171); “十二五”国家科技支撑计划(2012BAD21B04); 南京林业大学优秀博士学位论文创新基金项目
第一作者:杨世龙,博士生。*通信作者:徐莉,教授。E-mail: xuliqby@163.com。曹福亮,教授。E-mail:caofl@njfu.edu.cn。
引文格式:杨世龙,黄春丽,唐颖,等. 配位-有机溶剂法去除银杏叶初提物中银杏酚酸的研究[J]. 南京林业大学学报:自然科学版,2015,39(2):7-13.