马占相思和大叶相思优树组培不定根诱导研究

胡峰; 施琼; 黄烈健

doi:10.3969/j.issn.1000-2006.2015.02.010

胡峰,施琼,黄烈健

南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (02) : 57-62.

PDF(2095873 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(2095873 KB)

南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (02) : 57-62. DOI: 10.3969/j.issn.1000-2006.2015.02.010

研究论文

马占相思和大叶相思优树组培不定根诱导研究

胡峰¹, 施琼², 黄烈健^2*

作者信息 +

Induction of adventitious roots during tissue culture of Acacia mangium and A. auriculiformis elite trees

HU Feng¹, SHI Qiong², HUANG Liejian^2*

Author information +

文章历史 +

摘要

以10年生马占相思和大叶相思优良单株为研究对象,其茎段外植体通过初代培养和增殖培养后获得增殖芽,研究了生长素种类及浓度、基本培养基类型、蔗糖浓度和活性炭浓度对增殖芽生根的影响,分析了不同类型增殖培养基多次继代诱导的增殖芽对生根的影响。结果表明:马占相思的最佳生根培养基为1/2MS+0.5 mg/LIBA+0.5 mg/L NAA+3% 蔗糖,大叶相思最佳生根培养基为1/2MS+0.5 mg/L IBA+0.25 mg/L NAA+3% 蔗糖,二者的生根率均能达到93%以上。以马占相思和大叶相思增殖芽为生根材料,含低浓度细胞分裂素且添加活性炭的增殖培养基诱导时,两种相思的生根率均能达到90%以上,生根率随着继代次数的增加保持稳定,生根苗生长健壮。

Abstract

Ten years old Acacia mangium and A. auriculiformis elite individual trees were chosen as study materials in this experiment. Proliferated buds were obtained from stem explants being primarily cultured and proliferatively cultured. This study investigated factors related to rooting development of proliferated buds, such as type and concentration of auxins, type of basic medium, concentration of sucrose and active carbon. On the other hand, this experiment also studied the effects of proliferated buds induced by different medium on rooting rate. Results showed that the optimal rooting medium for A. mangium and A. auriculiformis was 1/2MS+IBA 0.5 mg/L+NAA 0.5 mg/L+sucrose 3% and 1/2MS+IBA 0.5 mg/L+NAA 0.25 mg/L+sucrose 3%, respectively. The rooting rate of both species could reach above 93%. Rooting rate of proliferated buds, after being cultured on proliferation medium with low concentration of cytokinin and active carbon, could reach above 90%, and remain stable with the increase of subculture time. Rooting seedlings grow strongly.

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用

胡峰,施琼,黄烈健. 马占相思和大叶相思优树组培不定根诱导研究[J]. 南京林业大学学报（自然科学版）. 2015, 39(02): 57-62 https://doi.org/10.3969/j.issn.1000-2006.2015.02.010

HU Feng, SHI Qiong, HUANG Liejian. Induction of adventitious roots during tissue culture of Acacia mangium and A. auriculiformis elite trees[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(02): 57-62 https://doi.org/10.3969/j.issn.1000-2006.2015.02.010

中图分类号： S722

参考文献

[1] Skolmen R G. Acacia(Acacia koa Gray)[J]. Biotechnology in Agriculture and Forestry, 1986(1):375-383.
[2] NFTA. Acacia mangium, A fast-growing tree for the humid tropics[J]. Nitrogen Fixing Tree Association, 1987(1): 87-104.
[3] 陆道调, 吴保国, 王希群, 等. 相思树种研究发展综述[J]. 福建林学院学报, 2004, 24(1): 92-96.Lu D D, Wu B G, Wang X Q, et al. A general review on the research development of Acacia spp.[J]. Journal of Fujian College of Forestry, 2004, 24(1): 92-96.
[4] 徐位力, 罗焕亮, 范恩友, 等. 二次正交旋转组合设计对马占相思组培增殖培养基的优化[J]. 广西植物, 2002, 22(6): 517-520. Xu L, Luo H L, Fan E Y, et al.Optimize the multiplication medium of Acacia mangium through the quadratic orthogonal rotation combination design[J]. Guihaia, 2002, 22(6): 517-520.
[5] Mandal P, Sinha Babu S P, Mandal N C. Antimicrobial activity of saponins from Acacia auriculiformis[J]. Fitoterapia,2005,76(5):462-465.
[6] Kabris N, Ray H N, Pal B C, et al. Pharmaceutical composition having virucidal and spermicidal activity[J]. United States Patent and Trademark Office Application,2008,30(1):97-100.
[7] 刘德朝. 马占相思扦插快繁育苗技术研究[J]. 防护林科技, 2007(6): 24-25.Liu D C.Rapid seedling cultivation technology for Acacia mangium cottage[J]. Protection Forest of Science and Technology, 2007(6): 24-25.
[8] 谢响明, 杨明嘉, 尚宇, 等. 相思树种的体外繁殖及基因工程研究进展[J]. 北京林业大学学报, 2006, 28(4): 148-154.Xie X M, Yang M J, Shang Y, et al.Advances in in vitro multiplication and genetic engineering in Acacia[J]. Journal of Beijing Forestry University, 2006, 28(4): 148-154.
[9] 裘珍飞, 曾炳山, 刘英. 马占相思优树组培早期增殖速率研究[J]. 林业科学研究, 2002, 15(1): 61-65.Qiu Z F, Zeng B S, Liu Y.Study on the early multiplicative ratio in vitro of Acacia mangium plus trees[J]. Forestry Research, 2002, 15(1): 61-65.
[10] 黄烈健, 陈祖旭, 张赛群, 等. 马占相思优树组培快繁技术研究[J]. 林业科学研究, 2012, 23(4): 227-230.Huang L J, Chen Z X, Zhang S Q, et al. Tissue culture technique of Acacia mangium elitie trees[J]. Forestry Research, 2012, 23(4): 227-230.
[11] Oliver M, Bon M C. Influence of auxins and darkness on in vitro rooting of micropropagated shoots from mature and juvenile Acacia mangium[J]. Plant Cell Tissue Organ Cult,2000,63:173-177.
[12] Pintu B. Rapid in vitro propagation of Acacia auriculiformis on solid and liquid media: role of organic additive, antioxidant and growth regulators[J]. Cibtech Journal of Bio-Protocols, 2013, 2(1): 39-49.
[13] Maity S, Ray S, Banerjee N. Enhanced in vitro shoot regeneration in multipurpose leguminous tree Acacia auriculiformis [J]. Physiology and Molecular Biology of Plants, 2007, 13(1):77-81.
[14] Girijashankar V.Micropropagation of multipurpose medicinal tree Acacia auriculiformis [J]. Journal of Medicinal Plants Research, 2011, 5(3): 462-466.
[15] 容世清, 曾少玲. 马占相思工厂化组培育苗技术的研究[J]. 热带林业, 1997, 25(2): 55-58.Rong S Q, Zeng S L. Research onindustrialized seedling techniques of tissue culture in Acacia mangium[J]. Tropical Forestry, 1997, 25(2):55-58.
[16] Rashmi M, Nanda, Premananda D, et al. In vitro clonal propagation of Acacia mangium Willd and its evaluation of genetic stability through RAPD marker[J]. Ann For Sci, 2004, 61:381-386.
[17] 裴红美, 韩科厅, 胡可, 等. 瓜叶菊“小丑”系列品种再生体系的研究[J]. 北京林业大学学报, 2011, 33(1): 108-114.Pei H M, Han K T, Hu K, et al.Plant regeneration of five color series of Senecio cruentus ‘Jester’[J]. Journal of Beijing Forestry University, 2011, 33(1): 108-114.
[18] 李校雨, 吕成群, 黄宝灵, 等. 马尾松组培苗不定根诱导及不定根解剖观察[J]. 西北林学院学报, 2009, 24(3): 80-84.Li X Y, Lv C Q, Huang B L, et al.Adventitious roots induction of Pinus massoniana shoots in test tubes and anatomical observation[J]. Journal of Northwest Forestry University, 2009, 24(3):80-84.
[19] Mccown B H, Sellmer J C. General media and vessels suitable for woody plant culture cell and tissue culture in forestry[J]. Forestry Sciences, 1987, 24: 14-16.
[20] 周小红, 张元莉, 李美平, 等. 杉木成年优树组培生根研究[J]. 南京林业大学学报: 自然科学版, 2013, 37(6):169-172.Zhou X H, Zhang Y L, Li M P, et al. Studies on factors influencing rooting efficiency from mature Chinese fir superior clones in vitro[J]. Journal of Nanjing Forestry University: Natural Sciences Edition, 2013, 37(6): 169-172.
[21] 刘用生, 曾兆云, 王秀芹, 等. 活性炭吸附作用的生物鉴定[J]. 植物生理学通讯, 1995, 31(2): 123-126.Liu Y S, Zeng Z Y, Wang X Q, et al. Biologic identification of absorption of active carbon[J]. Plant Physiology Communications, 1995, 31(2): 123-126.
[22] 秦莉, 吴小芹, 王张丽. 抗松材线虫病赤松组培苗的生根与移栽研究[J]. 南京林业大学学报: 自然科学版, 2011, 35(3): 89-92.Qin L, Wu X Q, Wang Z L. Study on rooting and transplanting of the tissue-cultured plantlets from Pinus densiflora with good resistance to pine wilt disease[J]. Journal of Nanjing Forestry University: Natural Sciences Edition, 2011, 35(3): 89-92.
[23] Custodio L, Matins M A, Romano A. Influence of sugars on in vitro rooting and acclimatization of carob tree[J]. Biologia Plantarum, 2004, 48(3): 469-472.

基金

收稿日期:2013-12-29 修回日期:2014-02-12
基金项目:“十二五”国家科技支撑计划(2012BAD01B0402)
第一作者:胡峰,硕士生。*通信作者:黄烈健,副研究员,博士。E-mail: 13802987948@163.com。
引文格式:胡峰, 施琼, 黄烈健. 马占相思和大叶相思优树组培不定根诱导研究[J]. 南京林业大学学报:自然科学版,2015,39(2):57-62.