Abstract: Xylo-oligosaccharides and cello-oligosaccharides are benificial for health and they are usually prepared from crops, such as corn cob. In this work, sand-fixing pioneer plant, Tamarix ramosissima, was used as the raw material for preparing the xylo-oligosaccharides and cello-oligosaccharides. The 100 g dried Tamarix ramosissima was pretreated by steam explosion at 200 ℃ for 180 s, 8.74 g xylo-oligosaccharides and 49.8% xylo-oligosaccharides yield were obtained. When the steam-exploded Tamarix ramosissima was cooked in 25% alkali charge(NaOH+Na₂S, 30% sulfidity)at a ratio of solid to liquid 1:6, 160 ℃ for 90 min, the lignin removal and cellulose recovery were 96.2% and 82.8%, respectively. Three-stage enzymatic hydrolysis of steam exploded and sulfate pretreated Tamarix ramosissima by deffcient β-glucosidase cellulase at dosage of 20 μmol/(min·g), substrate loading 5%, 50 ℃, pH 4.8 for 24 h, the yield of cello-oligosaccharides and the selectivity of cellulase to cello-oligosaccharides were 49.03% and 60.09%, respectively. The results showed that the steam explosion was suitable for xylo-oligosaccharides preparation from Tamarix ramosissima, and three-stage enzymatic hydrolysis was beneficial for cello-oligosaccharides preparation from pretreated Tamarix ramosissima.