碧桃花瓣转录组微卫星特征分析

doi:10.3969/j.issn.1000-2006.2015.03.007

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南京林业大学学报（自然科学版） ›› 2015, Vol. 58 ›› Issue (03): 34-38.doi: 10.3969/j.issn.1000-2006.2015.03.007

碧桃花瓣转录组微卫星特征分析

马秋月,廖卓毅,张得芳,戴晓港,陈赢男,李淑娴^*

南京林业大学南方现代林业协同创新中心,江苏南京 210037

出版日期:2015-05-30 发布日期:2015-05-30
基金资助:
收稿日期:2014-05-12 修回日期:2014-07-02
基金项目:国家林业公益性行业科研专项重大项目(201304102); 国家自然科学基金项目(31270711); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:马秋月,博士生; 廖卓毅,硕士生。*通信作者:李淑娴,研究员。E-mail: shuxianli@njfu.com.cn。
引文格式:马秋月,廖卓毅,张得芳,等. 碧桃花瓣转录组微卫星特征分析[J]. 南京林业大学学报:自然科学版,2015,39(3):34-38.

Deep sequenced-based transcriptome analysis of microsatellites in peach(Prunus persica cv. duplex) flowers

MA Qiuyue, LIAO Zhuoyi, ZHANG Defang, DAI Xiaogang, CHEN Yingnan, LI Shuxian^*

Co-Innovation Center for the Sustainable Forestry in Southern China,Nanjing Forestry University, Nanjing 210037, China

Online:2015-05-30 Published:2015-05-30

摘要/Abstract

摘要： 为开发碧桃转录组微卫星信息,利用454高通量测序技术,对其花瓣转录组序列进行SSR位点发掘,结果发现含SSR的序列4 705条,共得到5 668个SSR,平均每3.49 kb出现1个SSR。微卫星序列主要以三碱基重复为主,约占总数的42.66%。笔者共发现516种碱基重复基元,所占比例最高的为(AG/CT)_n(18.34%),其次是(AAG/CTT)_n(12.42%)。微卫星多为重复长度小于20 bp 的短序列, 长度大于20 bp 的微卫星仅占总数的12.13%。研究还发现碧桃花瓣微卫星的频率和长度呈显著负相关(P<0.05),相关系数为-0.246。

Abstract: To obtain information of Prunus persica cv. duplex, the transcriptome of peach flowers were sequenced by 454 high-throughput sequencing technology. A total of 5 668 SSRs were identified in 4 705 sequences, with an average density of one SSR per 3.49 kb. Tri-nucleotide repeats were the most abundant, accounted for 42.66% of all SSRs. Among all the 516 SSR motifs,(AG/CT)_n was the most frequent repeat motif(18.34%), followed by the(AAG/CTT)_n(12.42%). The microsatellites in length below 20 bp were in maximum proportion, while the microsatellites over 20 bp were only 12.13%. There were significant negative correlation(P<0.05)between the frequency of microsatellites and the length, the correlation coefficient was -0.246.

中图分类号:

Q948
Q78

马秋月,廖卓毅,张得芳,等. 碧桃花瓣转录组微卫星特征分析[J]. 南京林业大学学报（自然科学版）, 2015, 58(03): 34-38.

MA Qiuyue, LIAO Zhuoyi, ZHANG Defang, DAI Xiaogang, CHEN Yingnan, LI Shuxian. Deep sequenced-based transcriptome analysis of microsatellites in peach(Prunus persica cv. duplex) flowers[J].Journal of Nanjing Forestry University (Natural Science Edition), 2015, 58(03): 34-38.DOI: 10.3969/j.issn.1000-2006.2015.03.007.

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碧桃花瓣转录组微卫星特征分析

Deep sequenced-based transcriptome analysis of microsatellites in peach(Prunus persica cv. duplex) flowers

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