基于SSR标记的美洲黑杨杂交子代的鉴定

周文才; 侯静; 郭炜; 陈赢男; 万志兵; 尹佟明

doi:10.3969/j.issn.1000-2006.2015.03.009

基于SSR标记的美洲黑杨杂交子代的鉴定

周文才,侯静,郭炜,陈赢男,万志兵,尹佟明

南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (03) : 45-49.

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南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (03) : 45-49. DOI: 10.3969/j.issn.1000-2006.2015.03.009

研究论文

基于SSR标记的美洲黑杨杂交子代的鉴定

周文才^1,2, 侯静¹, 郭炜¹, 陈赢男¹,万志兵¹, 尹佟明^1*

作者信息 +

Identification of the true hybrids for Populus deltoides by using SSR markers

ZHOU Wencai^1,2, HOU Jing¹, GUO Wei¹, CHEN Yingnan¹, WAN Zhibing¹, YIN Tongming^1*

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摘要

杂交子代的纯度直接影响到遗传图谱的质量,因而在遗传图谱构建前要对杂交子代进行鉴定与选择。笔者从300对SSR标记中,筛选出11对能明确显示亲本来源的标记,利用这11对标记对380株美洲黑杨杂交子代进行了鉴定。结果表明:有372个个体因同时具有亲本各1条等位基因而被认定为亲子代可用于图谱构建,其余8个个体因产生了非亲本来源或额外的等位基因,这些个体可能源于花粉污染或染色体异常,在作图过程中宜从作图群体中排除。

Abstract

Since the purity of hybrids affects the quality of the genetic map, identification and selection of hybrids should be done before the genetic map construction. In this study, we screened 11 SSR pairs primer that could be used to track the kinship of the hybrids from a total 300 pairs primer. Subsequently, these primers were used to test 380 offsprings from a hybridization of Populus deltoides. Among which, 372 individuals were revealed to own complementary alleles for each parent, and they were identified as true hybrids for genetic map construction. Whereas the remaining eight individuals, they were observed to own non-parental alleles or extra parental alleles, suggesting these progeny were either resulted from pollen contamination or chromosomal abnormalities. Thus they were excluded from map construction.

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周文才,侯静,郭炜,陈赢男,万志兵,尹佟明. 基于SSR标记的美洲黑杨杂交子代的鉴定[J]. 南京林业大学学报（自然科学版）. 2015, 39(03): 45-49 https://doi.org/10.3969/j.issn.1000-2006.2015.03.009

ZHOU Wencai, HOU Jing, GUO Wei, CHEN Yingnan, WAN Zhibing, YIN Tongming. Identification of the true hybrids for Populus deltoides by using SSR markers[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(03): 45-49 https://doi.org/10.3969/j.issn.1000-2006.2015.03.009

中图分类号： S722 Q78

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基金

收稿日期:2014-07-24 修回日期:2015-01-19
基金项目:国家林业公益性行业科研重大项目(201304102); 国家自然科学基金项目(31400564,31400563); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:周文才,博士生。*通信作者:尹佟明,教授。E-mail: tmyin@njfu.com.cn
引文格式:周文才,侯静,郭炜,等. 基于SSR标记的美洲黑杨杂交子代的鉴定[J]. 南京林业大学学报:自然科学版,2015,39(3):45-49.