基于SSR标记的美洲黑杨杂交子代的鉴定

doi:10.3969/j.issn.1000-2006.2015.03.009

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2015, Vol. 58 ›› Issue (03): 45-49.doi: 10.3969/j.issn.1000-2006.2015.03.009

基于SSR标记的美洲黑杨杂交子代的鉴定

周文才^1,2, 侯静¹, 郭炜¹, 陈赢男¹,万志兵¹, 尹佟明^1*

1.南京林业大学林学院,江苏南京 210037;
2.江西省林业科学院,江西南昌 330032

出版日期:2015-05-30 发布日期:2015-05-30
基金资助:
收稿日期:2014-07-24 修回日期:2015-01-19
基金项目:国家林业公益性行业科研重大项目(201304102); 国家自然科学基金项目(31400564,31400563); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:周文才,博士生。*通信作者:尹佟明,教授。E-mail: tmyin@njfu.com.cn
引文格式:周文才,侯静,郭炜,等. 基于SSR标记的美洲黑杨杂交子代的鉴定[J]. 南京林业大学学报:自然科学版,2015,39(3):45-49.

Identification of the true hybrids for Populus deltoides by using SSR markers

ZHOU Wencai^1,2, HOU Jing¹, GUO Wei¹, CHEN Yingnan¹, WAN Zhibing¹, YIN Tongming^1*

1. College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2. Jiangxi Academy of Forestry, Nanchang 330032, China

Online:2015-05-30 Published:2015-05-30

摘要/Abstract

摘要： 杂交子代的纯度直接影响到遗传图谱的质量,因而在遗传图谱构建前要对杂交子代进行鉴定与选择。笔者从300对SSR标记中,筛选出11对能明确显示亲本来源的标记,利用这11对标记对380株美洲黑杨杂交子代进行了鉴定。结果表明:有372个个体因同时具有亲本各1条等位基因而被认定为亲子代可用于图谱构建,其余8个个体因产生了非亲本来源或额外的等位基因,这些个体可能源于花粉污染或染色体异常,在作图过程中宜从作图群体中排除。

Abstract: Since the purity of hybrids affects the quality of the genetic map, identification and selection of hybrids should be done before the genetic map construction. In this study, we screened 11 SSR pairs primer that could be used to track the kinship of the hybrids from a total 300 pairs primer. Subsequently, these primers were used to test 380 offsprings from a hybridization of Populus deltoides. Among which, 372 individuals were revealed to own complementary alleles for each parent, and they were identified as true hybrids for genetic map construction. Whereas the remaining eight individuals, they were observed to own non-parental alleles or extra parental alleles, suggesting these progeny were either resulted from pollen contamination or chromosomal abnormalities. Thus they were excluded from map construction.

中图分类号:

S722
Q78

周文才,侯静,郭炜,等. 基于SSR标记的美洲黑杨杂交子代的鉴定[J]. 南京林业大学学报（自然科学版）, 2015, 58(03): 45-49.

ZHOU Wencai, HOU Jing, GUO Wei, CHEN Yingnan, WAN Zhibing, YIN Tongming. Identification of the true hybrids for Populus deltoides by using SSR markers[J].Journal of Nanjing Forestry University (Natural Science Edition), 2015, 58(03): 45-49.DOI: 10.3969/j.issn.1000-2006.2015.03.009.

参考文献

[1] 何祯祥,施季森,邱进清,等.林木遗传图谱构建的技术与策略[J].浙江林学院学报,1998, 15(2):151-157.He Z X, Shi J S, Qiu J Q, et al. Technique and strategy of genome mapping in forest trees[J]. Journal of Zhejiang Forestry College, 1998, 15(2): 151-157.
[2] Frewen B E, Chen T H H, Howe G T, et al. Quantitative trait loci and candidate gene mapping of bud set and bud flush in Populus[J]. Genetics, 2000, 154(2): 837-845.
[3] Cervera M T, Storme V, Ivens B, et al. Dense genetic linkage maps of three Populus species(Populus deltoides, P. nigra and P. trichocarpa)based on AFLP and microsatellite markers[J]. Genetics, 2001, 158(2): 787-809.
[4] 李博.毛白杨与毛新杨转录组图谱构建及若干性状的遗传学联合分析[D].北京:北京林业大学,2009.Li B. Construction of transcriptome maps and QTL analyses of some complex traits by integrating genomics and transcriptomics for Populus tomentosa Carr. and P.tomentos×P.bolleana[D]. Beijing: Beijing Forestry University, 2009.
[5] 董维,苏舒,游录鹏,等.高粱F₆代群体分蘖数的QTL定位[J].南京林业大学学报:自然科学版,2013,37(2): 55-58.Dong W, Su S, You L P, et al. QTLs analysis of tillers number in F₆ sorghum population[J]. Journal of Nanjing Forestry University: Natural Sciences Edition, 2013, 2013,37(2):55-58.
[6] Yin T M, DiFazio S P, Gunter L E, et al. Large-scale heterospecific segregation distortion in Populus revealed by a dense genetic map[J]. Theoretical and Applied genetics, 2004, 109(3):451-463.
[7] Harald H, Goring H, Terwilliger J D. Linkage analysis in the presence of errors II: marker-locus genotyping errors modeled with hypercomplex recombination fractions[J]. American Journal of Human Genetics, 2000, 66(3): 1107-1118.
[8] Brzustowicz L M, Merette C, Xie X, et al. Molecular and statistical approaches to the detection and correction of errors in genotype databases[J]. American Journal of Human Genetics, 1993, 53(5): 1137.
[9] Lincoln S E, Lander E S. Systematic detection of errors in genetic linkage data[J]. Genomics, 1992, 14(3): 604-610.
[10] Abecasis G R, Cherny S S, Cardon L R. The impact of genotyping error on family-based analysis of quantitative traits[J]. European Journal of Human Genetics, 2001, 9(2): 130-134.
[11] Powell W, Machray G C, Provan J. Polymorphism revealed by simple sequence repeats[J]. Trends in Plant Science, 1996,1(7): 215-222.
[12] Maliepaard C, Jansen J, Van Ooijen J W. Linkage analysis in a full-sib family of an outbreeding plant species: overview and consequences for applications[J]. Genetics Research, 1997, 70(3): 237-250.
[13] Sundaram R M, Naveenkumar B, Biradar S K, et al. Identification of informative SSR markers capable of distinguishing hybrid rice parental lines and their utilization in seed purity assessment[J]. Euphytica, 2008, 163(2): 215-224.
[14] Xin Y Y, Zhang Z, Xiong Y P, et al. Identification and purity test of super hybrid rice with SSR molecular markers[J]. Rice Science, 2005, 12(1): 7-12.
[15] 王立新,常利芳,李宏博,等.小麦种子纯度的分子标记检测方法[J].麦类作物学报,2009,29(1):1-8.Wang L X, Chang L F, Li H B, et al. Method of wheat seeds purity testing by molecular markers[J]. Journal of Triticeae Crops, 2009, 29(1): 1-8.
[16] 吴明生,贾希海,田雷,等.利用SSR技术快速准确鉴定杂交玉米种子纯度[J].分子植物育种,2006,4(3):381-384.Wu M S, Jia X H, Tian L, et al. Rapid and reliable purity identification of F1 hybrids ofmaize(Zea may L.)using SSR markers[J]. Molecular Plant Breeding, 2006, 4(3)381-384.
[17] 王斯琪,唐诗哲,孔德仓,等.利用SSR标记进行枣树子代苗父本鉴定[J].园艺学报,2012,39(11):2133-2141.Wang S Q,Tang S Z,Kong D C, et al. Application of SSR markers for the identification of paternal parent for the seedlings of Chinese jujube[J]. Acta Horticulturae Sinica, 2012, 39(11): 2133-2141.
[18] 林二培,马海泉,樊民亮,等.百山祖冷杉SSR体系的建立及人工辅助授粉子代的初步鉴定[J].浙江农林大学学报,2011,28(2):234-240.Lin E P, Ma H Q, Fan M L, et al.An SSR reaction system for Abies beshanzuensis progeny with artificial pollination and progeny identification[J]. Journal of Zhejiang A&F University, 2011, 28(2): 234-240.
[19] 张红莲,李火根,胥猛,等.鹅掌楸属种及杂种的SSR分子鉴定[J].林业科学,2010,46(1):36-39.Zhang H L, Li H G, Xu M, et al. Identification of Liriodendron tulipifera, Liriodendron chinense and Hybrid Liriodendron using species-specific SSR markers[J].Scientia Silvae Sinicae, 2010,46(1):36-39.
[20] Tuskan G A, Gunter L E, Yang Z K, et al. Characterization of microsatellites revealed by genomic sequencing of Populus trichocarpa[J]. Canadian Journal of Forest Research, 2004, 34(1): 85-93.
[21] Yin T M, Zhang X Y, Gunter L E, et al. Microsatellite primer resource for Populus developed from the mapped sequence scaffolds of the Nisqually-1 genome[J]. New Phytologist, 2009, 181(2): 498-503.
[22] 房经贵,章镇,马正强,等.AFLP标记在两个芒果品种间杂交F₁代的多态性及分离方式[J].中国农业科学,2000,33(3):19-24.Fang J G, Zhang Z, Ma Z Q, et al.The polymorphism and segregation patterns of AFLP markers in the F₁ progenies from the cross of two Mango cultivars[J]. Scientia Agricultura Sinica, 2000,33(3):19-24.
[23] Moriya S, Iwanami H, Okada K, et al. A practical method for apple cultivar identification and parent-offspring analysis using simple sequence repeat markers[J]. Euphytica, 2011, 177(1): 135-150.
[24] Bradshaw Jr H D, Stettler R F. Molecular genetics of growth and development in Populus. I. Triploidy in hybrid poplars[J]. Theoretical and Applied Genetics, 1993, 86(2-3): 301-307.
[25] Kong F, Liu J, Chen Y, et al. Marker-aided selection of polyploid poplars[J]. BioEnergy Research, 2013, 6(3): 984-990.

相关文章 15

[1]	王改萍, 章雷, 曹福亮, 丁延朋, 赵群, 赵慧琴, 王峥. 红蓝光质对银杏苗木生长生理特性及黄酮积累的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 105-112.
[2]	宋子琪, 卞国良, 林峰, 胡凤荣, 尚旭岚. 流式细胞仪鉴定青钱柳倍性方法的建立及其应用[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 61-68.
[3]	孙旭高, 陶家璐, 谢微, 石洁, 张宝津, 邓小梅. 米老排优树组培技术体系优化研究[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 69-78.
[4]	顾宸瑞, 袁启航, 姜静, 穆怀志, 刘桂丰. 基于转录组测序的关联分析定位裂叶桦叶形调控基因[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 39-46.
[5]	杨蕴力, 曹俐, 王阳, 顾宸瑞, 陈坤, 刘桂丰. BpGLK1基因干扰表达对裂叶桦叶色及生长的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 18-28.
[6]	王伟, 邱志楠, 李爽, 白向东, 刘桂丰, 姜静. CRISPR/Cas9核糖核蛋白介导的无T-DNA插入的白桦BpGLK1精准突变[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 11-17.
[7]	国颖, 杨港归, 吴雨涵, 何杰, 何玉洁, 廖浩然, 薛良交. DNA甲基化调控植物组织培养过程的分子机制研究进展[J]. 南京林业大学学报（自然科学版）, 2023, 47(6): 1-8.
[8]	陈俊娜, 王晓宇, 陈晨, 彭辉武, 陈娟, 黄卫和, 喻方圆. BR对东京野茉莉种子中脂肪酸合成相关酶活性及油脂积累的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(6): 35-41.
[9]	宫楠, 祖鑫, 解志军, 朱长红, 李淑娴. 紫荆种子吸胀和层积过程中不同相态水分变化的核磁共振检测[J]. 南京林业大学学报（自然科学版）, 2023, 47(6): 42-50.
[10]	王章荣, 季孔庶, 徐立安, 邹秉章, 林能庆, 林景泉. 马尾松实生种子园营建技术、现实增益及多世代低成本经营新模式探讨[J]. 南京林业大学学报（自然科学版）, 2023, 47(6): 9-16.
[11]	欧阳, 欧阳芳群, 孙猛, 王超, 王军辉, 安三平, 王丽芳, 许娜, 王猛. 欧洲云杉无性系幼龄生长节律、年度和密度互作效应及选择策略[J]. 南京林业大学学报（自然科学版）, 2023, 47(6): 95-104.
[12]	罗芊芊, 李峰卿, 肖德卿, 邓章文, 王建华, 周志春. 两个南方红豆杉天然居群的交配系统分析[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 80-86.
[13]	郭伟, 韩秀, 张利, 王迎, 杜辉, 燕语, 孙忠奎, 张林, 李国华, 罗磊. 青檀扦插苗对不同氮素水平的形态、光合生理响应和转录组分析[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 87-96.
[14]	刘蓉, 吴德军, 王因花, 任飞, 李丽, 燕丽萍, 周晓锋. 白蜡花粉最佳离体萌发培养基筛选[J]. 南京林业大学学报（自然科学版）, 2023, 47(3): 70-76.
[15]	张港隆, 朱恩永, 刘丽婷, 杨嘉麒, 汪雁楠, 莫晓勇. 芒萁组培技术体系的优化[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 107-114.

基于SSR标记的美洲黑杨杂交子代的鉴定

Identification of the true hybrids for Populus deltoides by using SSR markers

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿