南京林业大学学报(自然科学版) ›› 2015, Vol. 39 ›› Issue (03): 45-49.doi: 10.3969/j.issn.1000-2006.2015.03.009
周文才1,2, 侯 静1, 郭 炜1, 陈赢男1,万志兵1, 尹佟明1*
出版日期:
2015-05-30
发布日期:
2015-05-30
基金资助:
ZHOU Wencai1,2, HOU Jing1, GUO Wei1, CHEN Yingnan1, WAN Zhibing1, YIN Tongming1*
Online:
2015-05-30
Published:
2015-05-30
摘要: 杂交子代的纯度直接影响到遗传图谱的质量,因而在遗传图谱构建前要对杂交子代进行鉴定与选择。笔者从300对SSR标记中,筛选出11对能明确显示亲本来源的标记,利用这11对标记对380株美洲黑杨杂交子代进行了鉴定。结果表明:有372个个体因同时具有亲本各1条等位基因而被认定为亲子代可用于图谱构建,其余8个个体因产生了非亲本来源或额外的等位基因,这些个体可能源于花粉污染或染色体异常,在作图过程中宜从作图群体中排除。
中图分类号:
周文才,侯静,郭炜,等. 基于SSR标记的美洲黑杨杂交子代的鉴定[J]. 南京林业大学学报(自然科学版), 2015, 39(03): 45-49.
ZHOU Wencai, HOU Jing, GUO Wei, CHEN Yingnan, WAN Zhibing, YIN Tongming. Identification of the true hybrids for Populus deltoides by using SSR markers[J].Journal of Nanjing Forestry University (Natural Science Edition), 2015, 39(03): 45-49.DOI: 10.3969/j.issn.1000-2006.2015.03.009.
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