南京林业大学学报(自然科学版) ›› 2015, Vol. 39 ›› Issue (03): 50-54.doi: 10.3969/j.issn.1000-2006.2015.03.010
王浩然1,2,邵志龙1,2,朱燕宇1,2,匡华琳1,2,黄敏仁1,2,陈 英1,2*
出版日期:
2015-05-30
发布日期:
2015-05-30
基金资助:
WANG Haoran1,2, SHAO Zhilong1,2, ZHU Yanyu1,2, KUANG Hualin1,2, HUANG Minren1,2, CHEN Ying1,2*
Online:
2015-05-30
Published:
2015-05-30
摘要: 以‘南林895’杨(Populus deltoides×P.euramericana cv ‘Nanlin895’)为材料,通过RACE-PCR技术,获得了NAC1基因的全长cDNA,命名为PdNAC1。该序列含有1个长906 bp的开放阅读框,编码302个氨基酸。该蛋白的分子质量为34.23 ku,等电点为7.44。分析其氨基酸序列发现,该蛋白具有典型NAC转录因子特征,NAM结构域位于11~135个氨基酸之间,含有A、B、C、D、E 5个亚结构域,该区域可以结合DNA; 而C端含有一个高度变异的转录激活区。利用杨树原生质体对PdNAC1蛋白进行细胞亚定位,结果发现PdNAC1不仅明显定位于细胞核中,而且在细胞膜上也有表达。但PdNAC1是否具有相应的跨膜结构域尚不明确。
中图分类号:
王浩然,邵志龙,朱燕宇,等. ‘南林895’杨PdNAC1基因克隆及蛋白的亚细胞定位[J]. 南京林业大学学报(自然科学版), 2015, 39(03): 50-54.
WANG Haoran, SHAO Zhilong, ZHU Yanyu, KUANG Hualin, HUANG Minren, CHEN Ying. Cloning and sub-cellular localization of PdNAC 1 in poplar ‘Nanlin895’[J].Journal of Nanjing Forestry University (Natural Science Edition), 2015, 39(03): 50-54.DOI: 10.3969/j.issn.1000-2006.2015.03.010.
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