Abstract: The regeneration system in this paper was established by using soft stem of Cryptomeria as explants. Using orthogonal experimental design, among which six Cryptomeria clones were used for the bud proliferation and three clones were used for rooting induction. Main conclusions were shown as follows:① Adventitious bud could be induced by six different clones on DCR basic medium supplement with different combinations of 6-BA and NAA. But there are differences in bud proliferation ability in the different clones. The bud proliferation coefficient was up to 5.00(Jing 1^#).② During the process of rooting culture of Cryptomeria clones, 1/2DCR medium was the optimum basic culture medium. And the rooting rate of three clones which had been experimented all reached the highest when added 0.10 mg/L IBA and 0.20 mg/L NAA. Among them, the rooting rate of 011# clone was up to 93.33%. The research will provide technical support for shortening the breeding cycle of the clones of Cryptomeria.