以聚乳酸为壁材,利用超声乳化同W1/O/W2复乳化方法相结合的方法,设计合成囊壁载有油溶性Fe3O4纳米粒子的磁性中空聚乳酸微囊。扫描电子显微镜分析(SEM)显示微囊外表面光滑,平均直径为1 μm; 透射电子显微镜(TEM)分析显示微囊中空结构明显,Fe3O4纳米粒子集中分布于囊壁结构。采用热重分析法(TGA)测定磁性微囊中Fe3O4质量分数高达12%。制得的磁性微囊具有较好的复溶性,在水溶液中能稳定分散,并具有较好的磁响应性,可望成为一种有效的磁靶向给药载体材料。
Abstract
The biodegradable magnetic poly lactic acid(PLA)microcapsules with Fe3O4 nanoparticles in the wall were designed and prepared based on the combination of ultrasonic emulsification technique and double emulsion-solvent evaporation method. Fe3O4 nanoparticles were prepared by coprecipitation method, and coated with undecylenic acid and oleic acid to improve its stability in dichloromethane. The structure and morphology of the magnetic PLA microcapsules were analyzed by scanning electron microcopy(SEM)and transmission electron microscopy(TEM). Moreover, the thermal properties of the magnetic PLA microcapsules were measured by thermogravimetric analysis(TGA). As demonstrated by experimental results, the diameter of the uniform magnetic microcapsules was about 1 μm, and the magnetic PLA microcapsules could be easily separated from aqueous solution by an external magnetic field.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Pisani E, Tsapis N, Galaz B, et al. Perfluorooctyl bromide polymeric capsules as dual contrast agents for ultrasonography and magnetic resonance imaging[J]. Advanced Functional Materials, 2008, 18(19): 2963-2971.
[2] Zhang C, Hu C, Zhao Y, et al. Encapsulation of laccase in silica colloidosomes for catalysis in organic media[J]. Langmuir, 2013, 29(49): 15457-15462.
[3] Shin M J, Kim J G, Shin J S. Microencapsulation of imidazole curing agents by spray-drying method using W/O emulsion[J]. Journal of Applied Polymer Science, 2012, 126(S2):E108-E115.
[4] Chandrawati R, Odermatt P D, Chong S F, et al. Triggered cargo release by encapsulated enzymatic catalysis in capsosomes[J]. Nano letters, 2011, 11(11): 4958-4963.
[5] Yan Y, Bjo rnmalm M, Caruso F. Assembly of layer-by-layer particles and their interactions with biological systems[J]. Chemistry of Materials, 2013, 26(1): 452-460.
[6] Domaratzki R E, Ghanem A. Encapsulation and release of cladribine from chitosan nanoparticles[J]. Journal of Applied Polymer Science, 2013, 128(3): 2173-2179.
[7] Pichayakorn W, Boonme P. Evaluation of cross-linked chitosan microparticles containing metronidazole for periodontitis treatment[J]. Materials Science and Engineering: C, 2013, 33(3): 1197-1202.
[8] Hui P C L, Wang W Y, Kan C W, et al. Preparation and characterisation of chitosan microcapsules loaded with cortex moutan[J]. International Journal of Biological Macromolecules, 2013, 55: 32-38.
[9] Molino Cornejo J J, Daiguji H, Takemura F. Factors affecting the size and uniformity of hollow poly(lactic acid)microcapsules fabricated from microbubble templates[J]. The Journal of Physical Chemistry B, 2011, 115(47): 13828-13834.
[10] Hwang T, Park T J, Koh W G, et al. Fabrication of nano-scale liposomes containing doxorubicin using Shirasu porous glass membrane[J]. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2011, 392(1): 250-255.
[11] Stride E, Edirisinghe M. Novel microbubble preparation technologies[J]. Soft Matter, 2008, 2(12): 2350-2359.
[12] Eisenbrey J R, Burstein O, Kambhampati R. Development and optimization of a doxorubicin loaded poly(lactic acid)contrast agent for ultrasound directed drug delivery[J]. Journal of Control Release, 2010, 143(1): 38-44.
[13] Liu R, Ma G H, Meng F T. Preparation of uniform-sized PLA microcapsules by combining Shirasu porous glass membrane emulsification technique and multiple emulsion-solvent evaporation method[J]. J. Control. Release, 2005, 103(1): 31-43.
[14] Daiguji H, Takada S, Cornejo J J M, et al. Fabrication of hollow poly(lactic acid)microcapsules from microbubble templates[J]. The Journal of Physical Chemistry: B, 2009, 113(45):15002-15009.
[15] Sun Y, Zheng Y, Ran H, et al. Superparamagnetic PLGA-iron oxide microcapsules for dual-modality US/MR imaging and high intensity focused US breast cancer ablation[J]. Biomaterials, 2012, 33(24): 5854-5864.
[16] Chow A M, Chan K W Y, Cheung J S, et al. Enhancement of gas-filled microbubble R2* by iron oxide nanoparticles for MRI[J]. Magnetic Resonance in Medicine, 2010, 63(1): 224-229.
[17] Xu B, Dou H, Tao K, et al. “Two-in-One” fabrication of Fe3O4/MePEG-PLA composite nanocapsules as a potential ultrasonic/MRI dual contrast agent[J]. Langmuir, 2011, 27(19): 12134-12142.
[18] Zhao H, Saatchi K, Häfeli U O. Preparation of biodegradable magnetic microspheres with poly(lactic acid)-coated magnetite[J]. Journal of Magnetism and Magnetic Materials, 2009, 321(10): 1356-1363.
[19] Kanouni M, Rosano H L, Naouli N. Preparation of a stable double emulsion(W 1/O/W 2): role of the interfacial films on the stability of the system[J]. Advances in Colloid and Interface Science, 2002, 99(3): 229-254.
基金
收稿日期:2014-05-09 修回日期:2014-10-11基金项目:江苏省基础研究计划项目(BK20130969); 江苏高校优势学科建设工程资助项目(PAPD); 2013年南京林业大学大学生实践创新训练计划项目 第一作者:徐鹏,讲师。*通信作者:张蕤,教授。E-mail:zhangrui@njfu.edu.cn。引文格式:徐鹏,陈懿玺,唐进根,等. 载Fe3O4纳米粒子磁性PLA微囊的制备[J]. 南京林业大学学报:自然科学版,2015,39(4)
PDF(1929304 KB)
