不同提取方法对葡萄渣凝缩类单宁化学组成的影响

doi:10.3969/j.issn.1000-2006.2015.04.018

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南京林业大学学报（自然科学版） ›› 2015, Vol. 58 ›› Issue (04): 105-110.doi: 10.3969/j.issn.1000-2006.2015.04.018

不同提取方法对葡萄渣凝缩类单宁化学组成的影响

兰平¹, 黄润州^1*, 周定国¹, BROSSE Nicolas², CHRUSCIEL Laurent²

1.南京林业大学材料科学与工程学院,江苏南京 210037;
2.Laboratoired’Etude et de Recherche sur le Materiau Bois, Faculte des Sciences et Techniques, Universite de Lorraine, Nancy 54500, France

出版日期:2015-07-31 发布日期:2015-07-31
基金资助:
收稿日期:2014-06-16 修回日期:2014-12-10基金项目:国家自然科学基金项目(31400502, 31300482); 江苏省自然科学基金项目(BK20130966); CPER 2007-2013 “Structuration du P?le de Compétitivité Fibres Grand’Est”(French Competitiveness Fibre Cluster) 第一作者:兰平, 实验师, 博士。*通信作者:黄润州, 讲师,博士。

Influence of different extraction methods on the chemical composition of grape pomace condensed tannins

LAN Ping¹, HUANG Runzhou^1*, ZHOU Dingguo¹, BROSSE Nicolas², CHRUSCIEL Laurent²

1.College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China;
2.Laboratoired’Etude et de Recherche sur le Materiau Bois, Faculte des Sciences et Techniques, Universite de Lorraine, Nancy 54500, France

Online:2015-07-31 Published:2015-07-31

摘要/Abstract

摘要： 分别采用酸沉淀法和冷冻干燥法从葡萄渣中提取凝缩类单宁,借助碳核磁共振波谱法(¹³C-NMR)和基质辅助激光解吸/电离飞行时间质谱法(MALDI-TOF),分析不同的提取方法对凝缩类单宁提取物化学结构和组成的影响。结果表明:这两种单宁提取物以原花青定类凝缩单宁为主,少量的原花青定上含有葡萄糖基取代基和没食子酸取代基,单宁单体间以C4-C8键连接; 酸化凝缩类单宁分子聚合度高于冻干凝缩类单宁; 同时,酸化凝缩类单宁的中碳水化合物含量低于冻干凝缩类单宁,但儿茶酸含量高于冻干凝缩类单宁。

Abstract: Condensed tannins were extracted from grape pomace by acid precipitation and lyophilisation, respectively. Influence of the recovery methods on the chemical structure and composition of tannin extracts was investigated by using Carbon Nuclear Magnetic Resonance(¹³C-NMR)and Matrix-Assisted Laser Desorption/Ionization Time of Flight(MALDI-TOF)Mass Spectrometry. The results showed that the procyanidin units played a dominait role in two kinds of condensed tannin extracts, and small proportion glucose and gallic acid substitutions bonded in procyanidin units which was linked together by C4-C8 bond. The polymerization degree of acidified condensed tannins was higher than that of lyophilized condensed tannins. In addition, relatively low carbonhydrate and high catechinc acid content in acidified condensed tannins were observed compared with lyophilized condensed tannins. Different extraction methods had great influence on the chemical composition of grape pomace tannin extracts.

中图分类号:

TQ432.9

兰平,黄润州,周定国,等. 不同提取方法对葡萄渣凝缩类单宁化学组成的影响[J]. 南京林业大学学报（自然科学版）, 2015, 58(04): 105-110.

LAN Ping, HUANG Runzhou, ZHOU Dingguo, BROSSE Nicolas, CHRUSCIEL Laurent. Influence of different extraction methods on the chemical composition of grape pomace condensed tannins[J].Journal of Nanjing Forestry University (Natural Science Edition), 2015, 58(04): 105-110.DOI: 10.3969/j.issn.1000-2006.2015.04.018.

参考文献

[1] Pizzi A. Condensed tannins for adhesives[J].Ind Eng Chem Prod Res Dev, 1982, 21(3): 359-369.
[2] Vázquez G, Freire S, González J, et al. Characterization of Pinus pinaster bark and its alkaline extracts by diffuse reflectance fourier transform infrared(DRIFT)spectroscopy[J]. Holz als Roh-und Werkstoff, 2000, 58(1): 57-61.
[3] Panamgama L A. Polyphenolic extracts of Pinus radiata bark and networking mechanisms of additive-accelerated polycondensates[J]. Journal of Applied Polymer Science, 2007,103(4):2487-2493.
[4] Fradinho D M, Neto C P, Evtuguin D, et al. Chemical characterization of bark and of alkaline bark extracts from maritime pine grown in Portugal[J]. Industrial Crops and Products, 2002, 16(1): 23-32.
[5] Ping L, Brosse N, Chrusciel L, et al. Extraction of condensed tannins from grape pomace for use as wood adhesives[J]. Industrial Crops and Products, 2011, 33(1): 253-257.
[6] Ping L, Pizzi A, Zhou D G, et al. Condensed tannins extraction from grape pomace: characterization and utilization as wood adhesives for wood particleboard[J]. Industrial Crops and Products, 2011, 34(1): 907-914.
[7] Fu C, Loo A E K, Chia F P P, et al. Oligomeric proanthocyanidins from mangosteen pericarps[J]. Journal of Agriculture and Food Chemistry, 2007, 55(19): 7689-7694.
[8] Hoong Y B, Pizzi A, Tahir P M, et al. Characterization of Acacia mangium polyflavonoid tannins by MALDI-TOF mass spectrometry and CP-MAS ¹³C NMR[J]. European Polymer Journal, 2010, 46(6): 1268-1277.
[9] Navarrete P, Pizzi A, Pasch H, et al. MALDI-TOF and ¹³C NMR characterization of maritime pine industrial tannin extract[J]. Industrial Crops and Products, 2010, 32(2): 105-110.
[10] Pizzi A. Advanced wood adhesive technology[M]. New York: Marcel Dekker Inc., 1994, 156-168.
[11] Sears K D, Casebier R L, Hergert H L, et al. The structure of catechinic acid: a base rearrangement product of catechin[J]. J Org Chem, 1974, 39(22): 3244-3247.
[12] Kiatgrajai P, Wellons J D, Gollob L, et al. Kinetics of epimerization of(+)-catechin and its rearrangement to catechinic acid[J]. J Org Chem, 1982, 47(5): 2910-2912.
[13] Monagasa M, López J E Q, Cordovésa C G, et al. MALDI-TOF MS analysis of plant proanthocyanidins[J]. Journal of Pharmaceutical and Biomedical Analysis, 2010, 51(2):358-372.
[14] Reed J D, Krueger C G, Vestling M M. MALDI-TOF mass spectrometry of oligomeric food polyphenols[J]. Phytochemistry, 2005, 66(18): 2248-2263.
[15] Ricardo-Da-Silva J M, Riguad J, Cheynier V, et al. Procyanidin dimers and trimers from grape seeds[J]. Phytochemistry, 1991, 30(4): 1259-1264.
[16] Hoong Y B, Paridah M T, Luqman C A, et al. Fortification of sulfited tannin from the bark of Acacia mangium with phenol-formaldehyde for use as plywood adhesive[J]. Industrial Crops and Products, 2009, 30(3): 416-421.
[17] Pash H, Pizzi A, Rode K. MALDI-TOF mass spectrometry of polyflavonoid tannins[J]. Polymer, 2001, 42(18): 7531-7539.

不同提取方法对葡萄渣凝缩类单宁化学组成的影响

Influence of different extraction methods on the chemical composition of grape pomace condensed tannins

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