采用低聚木糖生产废渣替代麸皮固态发酵制备β-甘露聚糖酶,研究了碳源种类和组成、诱导物、氮源种类和组成对黑曲霉固态发酵制备β-甘露聚糖酶的影响。结果表明:低聚木糖废渣可部分替代麸皮固态发酵制备β-甘露聚糖酶。以低聚木糖废渣和麸皮质量比4:6为复合碳源,2%的硫酸铵为氮源,黑曲霉在添加3%魔芋粉的培养基中于30 ℃条件下固态发酵5 d,制备的β-甘露聚糖酶、β-甘露糖苷酶活力分别为341.8、7.02 μmol/(min·g),可溶性蛋白含量为8.40 mg/g。
Abstract
The residues from xylo-oligosaccharides production was applied for β-mannanase preparation instead of wheat bran, and effects of different kinds and ratio of carbon sources, inducer, nitrogen sources and loading on solid state fermentation for β-mannanase preparation by Aspergillus niger were investigated in this work. Residues of xylo-oligosaccharides production could be instead of wheat bran partly for β-mannanase preparation. Solid state fermentation of residues of xylo-oligosaccharides production and bran by A. niger for five days, which the mass ratio of residues of xylo-oligosaccharides production to wheat bran was 4:6, 2% of ammonia sulfate and 3% of konjac flour loading in the medium, the β-mannanase activity and β-mannosidase activity were 341.8 and 7.02 μmol/(min·g), respectively, and soluble protein content was 8.40 mg/g.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Schimbug D, Salmann M. Enzyme handbook(4)[M]. Berlin Heideberg: Springer-Verlag, 1991: 1-5.
[2] Clarke J H, Davidson K, Rixon J E, et al. A comparison of enzyme-aided of softwood paper pulp using combinations of xylanase,mannanase and Alpha-galactosidase[J]. Appl Microbiol Biotechnol, 2000, 53(6):661-667.
[3] 李希明,陈勇,谭云贤,等.生物破胶酶研究及应用[J].石油钻采工艺,2006,28(2):52-54.Li X M, Chen Y, Tan Y X, et al. Study and application of biology viscosity break ferment[J]. Oil Drilling and Production Technology, 2006, 28(2): 52-54.
[4] 陈建红,周海燕,曾分有,等. 魔芋葡甘露低聚糖对小鼠抗氧化酶活性的影响[J]. 氨基酸和生物资源,2006,28(1):40-41.Chen J H, Zhou H Y,Zeng F Y, et al. Effect of konjac oligosaccharifides on antioxidative enzymes in mice[J]. Amino Acids and Bioticresources, 2006, 28(1):40-41.
[5] Moreira L R S, Filho E X F. An overview of mannan structure and mannan-degrading enzyme systems[J]. Applied Microbiology and Biotechnology, 2008, 79(2):165-178.
[6] Chen M, Xia L M, Xue P J. Enzymatic hydrolysis of corncob and ethanol production from cellulosic hydrolysate[J]. International Biodeterioration and Biodegradation, 2007, 59:85-89.
[7] 勇强,范一民,徐勇,等. 低聚木糖生产废渣纤维素酶诱导和水解特性的研究[J]. 林产化学与加工业,2005,25(3):1-4.Yong Q, Fan Y M,Xu Y, et al. Characteristics of cellulase inducement and hydrolysis by residue of xylooligosaccharide production[J]. Chemistry and Industry of Forest Products, 2005, 25(3):1-4.
[8] 欧阳嘉,李建明,李鑫,等. 酶法水解低聚木糖生产废渣工艺的研究[J]. 太阳能学报,2010,31(2):263-267.Ouyang J, Li J M, Li X, et al. An experimental research on enzymatic hydrolysis of waster residue of xylooligosaccharide[J]. Acta Energiae Solaris Sinica, 2010, 31(2):263-267.
[9] 黄达明,吴其飞,陆建明,等. 固态发酵技术及其设备的研究进展[J].食品与发酵工业,2003,29(6):87-91.Huang D M, Wu Q F, Lu J M, et al. Current research progress of solid state fermentation technology and equipment[J]. Food and Fermentation Industries, 2003, 29(6):87-91.
[10] H?lker U, H?fer M, Lenz J. Biotechnological advantages of laboratory-scale solid-state fermentation with fungi[J]. Appl Microbiol Biotechnol, 2004, 64(2):175-186.
[11] 王静,李鑫,朱均均,等. 培养基组成对里氏木霉合成β-甘露聚糖酶的影响[J]. 南京林业大学学报:自然科学版,2013,37(1):101-104.Wang J, Li X, Zhu J J. et al. Effects of medium components on β-mannanase production by Trichoderma reesei[J]. Journal of Nanjing Forestry University: Natural Sciences Edition, 2013, 37(1):101-104.
[12] Adney B, Baker J. Biomass analysis technology team laboratory analysis procedure[R].Golden, CO, USA:National Renewable Energy Laboratory,1996.
[13] Mudau M M. The production, purification and characterization of endo-1,4-β-mannanase from newly isolated strains of Scopulariopsis candida[D]. Bloemfontein: Faculty of Natural and Agricultural Sciences, 2006.
[14] 杨淑蕙. 植物纤维化学[M]. 北京:中国轻工业出版社,2001:6-7.Yang S H. Chemistry of plantfibre[M]. Beijing: China Light Industry Press, 2001:6-7.
[15] 李剑芳,邬敏辰,夏文水. β-甘露聚糖酶高产菌株选育及产酶条件的研究[J].食品与发酵工业,2005,31(9):9-13.Li J F, Wu M C,Xia W S. Breeding of acidic β-Mannanase overproducing strain and studies on its enzyme production conditions[J]. Food and Fermentation Industries Editorial Staff, 2005, 31(9):9-13.
基金
收稿日期:2014-06-13 修回日期:2015-04-07基金项目:国家林业公益性行业科研专项项目(201404615); 江苏省六大人才高峰项目 第一作者:张晓燕,硕士生。*通信作者:勇强,教授。E-mail:swhx@njfu.com.cn。引文格式:张晓燕,李鑫,王静,等. 碳源和氮源对固态发酵制备β-甘露聚糖酶的影响[J]. 南京林业大学学报:自然科学版,2015,39(4):143-148.
PDF(1815594 KB)
