为研究巨紫荆种子萌发期对水分胁迫的耐受性,以80 ℃热水处理12 h,150 mg/L赤霉素浸种12 h,低温沙藏1个月,打破休眠后的巨紫荆种子为材料,采用培养皿纱布滤纸萌发的方法研究了不同浓度聚乙二醇(PEG)溶液对其种子萌发、生长、保护酶活性和有机渗透调节物质含量的影响,并对种子的萌发率、胚根长度等与PEG浓度进行了回归分析。结果表明:①PEG胁迫下,随着干旱胁迫的加剧,巨紫荆种子的发芽率、发芽指数、活力指数、胚根长度呈先升高后下降趋势,说明低浓度(质量分数5%)PEG胁迫可以促进巨紫荆种子的萌发和生长,而高浓度(质量分数10%、20%、30%)时则具有抑制作用; ②随着PEG浓度的升高,巨紫荆种子中3种有机调节物质(可溶性糖、可溶性蛋白、游离脯氨酸)含量、SOD活性均呈现不同程度的先下降后升高趋势, POD活性则呈现先升高后下降趋势。由此可见,巨紫荆种子可通过增加保护酶活性和体内渗透调节物质来适应水分胁迫环境,从而使受抑制程度降到最低。
Abstract
The purpose of this study was to understand the drought tolerance of Cercis gigante seed in germination stage.The seeds were soaked at 80 ℃ hot water for 12h,then treated with 150 mg/L gibberellin for 12h,and finally stratified at low temperature for one month. After dormancy breaking,the seeds of Cercis gigante seeds were germinated on filter paper in Petri dish gauze under different concentration of PEG solution. Effects of drought stress on seed germination percentage, seedling growth, protective enzyme activity and organic osmoregulation substances were studied.A regression analysis was conducted between PEG concentration and the germination rate, radicle length and so on. Results showed that: ① Under PEG stress, the seed germination rate, germination index, vigor index, radicle and hypocotyl length showed a trend of rising first and then decline with the aggravating of drought stress,indicating that low concentration(5%)of PEG could promote the seeds germination and growth, whereas high concentrations(10%,20%, 30%)had the inhibition effects. ② With the increase of PEG concentration, contents of three kinds of organic regulation substances(soluble sugar, soluble protein and free proline)and SOD activity in Cercis gigante seedsall showed a trend of decline first and then rising.POD activity showed a trend of rising first and then decline.Thus it could be seen that the drought tolerance of Cercis gigante seeds could be improved by increasing protective enzyme activities and contents of osmotic regulation substances, making the inhibition degree to the minimum.
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基金
收稿日期:2014-07-05 修回日期:2014-09-10基金项目:江苏省林业三项工程项目(lysx[2013]24) 第一作者:凌敏,硕士生。*通信作者:王良桂,教授。E-mail: wlg@njfu.com.cn。引文格式:凌敏,杨秀莲,王良桂. PEG模拟干旱胁迫对巨紫荆种子萌发及生长生理的影响[J]. 南京林业大学学报:自然科学版,2015,39(4):168-172.
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