斑翅果蝇幼虫人工饲料的优化配方

吴军; 骆亚琴; 廖太林; 曾虹; 师振华; 陈集翰; 纪睿; 安榆林

doi:10.3969/j.issn.1000-2006.2015.04.031

斑翅果蝇幼虫人工饲料的优化配方

吴军,骆亚琴,廖太林,曾虹,师振华,陈集翰,纪睿,安榆林

南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (04) : 178-180.

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南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (04) : 178-180. DOI: 10.3969/j.issn.1000-2006.2015.04.031

研究简报

斑翅果蝇幼虫人工饲料的优化配方

吴军¹,骆亚琴¹,廖太林^1*,曾虹¹,师振华¹,陈集翰¹,纪睿¹,安榆林²

作者信息 +

The orthogonal optimization of artificial diet for larvae of Drosophila suzukii Matsumura

WU Jun¹, LUO Yaqin¹, LIAO Tailin^1*, ZENG Hong¹, SHI Zhenhua¹, CHEN Jihan¹, JI Rui¹, AN Yulin²

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摘要

为规模化人工饲养斑翅果蝇,以斑翅果蝇幼虫存活率、幼虫历期、蛹平均质量以及羽化率作为考察指标,采用正交试验设计,对其幼虫人工饲料3种主要成分(玉米颗粒、蔗糖、酵母浸粉)用量进行优化筛选。结果表明:玉米颗粒对斑翅果蝇幼虫存活率有显著影响,蔗糖对蛹平均质量有显著影响,酵母浸粉对幼虫历期有显著影响; 斑翅果蝇幼虫基础人工饲料中的最优质量配比为m(玉米颗粒):m(蔗糖):m(酵母浸粉)=9:16:3。

Abstract

In order to provide the basis for large-scale artificial breeding of Drosophila suzukii Matsumura, the composition of artificial diet for larvae was optimized by orthogonal design with 3 factors and 3 levels. Corn particle, sucrose and yeast extract were selected as the three key factors to determine their effects on the survival rate of larvae, larval developmental duration, mean pupal weight and emergence rate. Corn particle had significant effect on the survival rate of larvae. Sucrose had significant effect on mean pupal weight, and yeast extract had significant effect on larval developmental duration. As the results, the optimized weight ratio of corn particle to sucrose and yeast extract was 9:16:3.

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吴军,骆亚琴,廖太林,曾虹,师振华,陈集翰,纪睿,安榆林. 斑翅果蝇幼虫人工饲料的优化配方[J]. 南京林业大学学报（自然科学版）. 2015, 39(04): 178-180 https://doi.org/10.3969/j.issn.1000-2006.2015.04.031

WU Jun, LUO Yaqin, LIAO Tailin, ZENG Hong, SHI Zhenhua, CHEN Jihan, JI Rui, AN Yulin. The orthogonal optimization of artificial diet for larvae of Drosophila suzukii Matsumura[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(04): 178-180 https://doi.org/10.3969/j.issn.1000-2006.2015.04.031

中图分类号： S763

参考文献

[1] Hauser M. A historic account of the invasion of Drosophila suzukii(Matsumura)(Diptera: Drosophilidae)in the continental United States, with remarks on their identification [J]. Pest Management Science, 2011, 67(11):1352-1357.
[2] Lee J C, Bruck D J, Dreves A J, et al. In Focus: Spotted wing drosophila,Drosophila suzukii, across perspectives [J]. Pest Management Science, 2011, 67: 1349-1351.
[3] 吴军,廖太林,孙鹏,等. 斑翅果蝇生物学特性研究[J]. 植物检疫,2013,27(5):36-41.Wu J, Liao T L, Sun P, et al. Bionomics of Drosophila suzukii Matsumura,1931(Diptera: Drosophilidae)[J]. Plant Quarantine, 2013, 27(5):36-41.
[4] Seljak G. Diagnostics: PM 7/115(1)Drosophila suzukii[J/OL]. EPPO Bulletin, 2013, 43(3):417-424.
[2014-02-18]. http://onlinelibrary.wiley.com/doi:10.1111/epp.12059.pdf.
[5] Lee J C, Bruck D J, Curry H. The susceptibility of small fruits and cherries to the spotted-wing drosophila, Drosophila suzukii[J]. Pest Management Science, 2011, 67(11): 1358-1367.
[6] Landolt P J, Adams T, Rogg H.Trapping spotted wing drosophila, Drosophila suzukii(Matsumura)(Diptera: Drosophilidae), with combinations of vinegar and wine, and acetic acid and ethanol[J]. Journal of Applied Entomology, 2012, 95(136):148-154.
[7] 廖太林,崔平福,吴军,等. 斑翅果蝇的 PCR-RFLP快速鉴定方法[J]. 植物检疫,2013,27(1):25-29.Liao T L, Cui P F, Wu J, et al. Rapid identification of Drosophila suzukii by PCR-RFLP[J]. Plant Quarantine, 2013, 27(1):25-29.
[8] 张会宜.果蝇饲料玉米粉培养基配方的改进[J]. 唐山师范学院学报,2005,27(5):46-47, 64.Zhang H Y. Improvement on culture medium of corn powder for Drosophila[J]. Journal of Tangshan Teachers College, 2005, 27(5):46-47,64.
[9] 卞立红,袁红梅,汪洋,等. 不同配方培养基对果蝇生长和繁殖的影响[J]. 天津农业科学,2012,18(3):144-146,160.Bian L H, Yuan H M, Wang Y, et al. Effect of different culture medium on growth and reproduction of Drosophila[J]. Tianjin Agricultural Sciences, 2012, 18(3): 144-146,160.
[10] 王转斌, 曲志才. 培养果蝇的最佳生态因子研究[J]. 菏泽学院学报,2006,28(5):112-114.Wang Z B, Qu Z C. An investigation on optimal ecological factors for breeding fruit fly[J]. Journal of Heze University, 2006, 28(5): 112-114.
[11] 王翔韩,吴伟坚,李梅辉,等. 瓜实蝇幼虫人工饲料设计及其优化[J]. 环境昆虫学报,2012,34(1):115-119. Wang X H,Wu W J,Li M H,et al. The orthogonal optimization of artificial diet for larvae of Bactrocera cucurbitae(Coquillett)[J]. Journal of Environmental Entomology,2012, 34(1): 115-119.
[12] 林明光,汪兴鉴,张艳,等. 橘小实蝇、瓜实蝇和南亚果实蝇人工饲料的优化[J]. 应用昆虫学报,2013,50(4):1115-1125.Lin M G, Wang X J, Zhang Y, et al. Optimization of artificial diets for mass rearing of Bactrocera dorsalis,Bactrocera cucurbitae and Bactrocera tau [J]. Chinese Journal of Applied Entomology, 2013, 50(4):1115-1125.
[13] 陈毅超,陈玲,商晗武. 混料设计在优化橘小实蝇人工饲料中的应用[J]. 中国生物防治学报,2013,29(3):354-361.Chen Y C, Chen L, Shang H W.Application of a mixture design to optimize the artificial diet for the oriental fruit fly, Bactrocera dorsalis [J]. Chinese Journal of Biological Control, 2013, 29(3): 354-361.
[14] 李丽丽. 橘小实蝇大量饲养技术优化[D]. 福州:福建农林大学,2009. Li L L.Technique optimization of mass rearing oriental fruit fly, Bactrocera dorsalis (Hendel)[D]. Fuzhou: Fujian Agriculture and Forestry University, 2009.

基金

收稿日期:2014-07-10 修回日期:2014-12-10基金项目:江苏出入境检验检疫局科技资助项目(2013KJ51) 第一作者:吴军,农艺师。*通信作者:廖太林,高级工程师,博士。E-mail: liaotl@jsciq.gov.cn。引文格式:吴军,骆亚琴,廖太林,等. 斑翅果蝇幼虫人工饲料的优化配方[J]. 南京林业大学学报:自然科学版,2015,39(4):178-180.