板栗愈伤组织对栗疫菌Cp-毒素的抗性响应

韩珊; 朱天辉; 谯天敏; 李姝江; 汪菊; 张博阳

doi:10.3969/j.issn.1000-2006.2015.05.001

韩珊,朱天辉,谯天敏,李姝江,汪菊,张博阳

南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (05) : 1-6.

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南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (05) : 1-6. DOI: 10.3969/j.issn.1000-2006.2015.05.001

专题报道

板栗愈伤组织对栗疫菌Cp-毒素的抗性响应

韩珊^1,2,朱天辉^1,2*,谯天敏³,李姝江^1,2,汪菊²,张博阳²

作者信息 +

Resistance responses of Castanea mollissima callus to Cp-toxin under Cryphonectria parasitica stress

HAN Shan^1,2, ZHU Tianhui^1,2*, QIAO Tianmin³, LI Shujiang^1,2, WANG Ju², ZHANG Boyang²

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摘要

为了探讨板栗愈伤组织对栗疫病的抗性机制,采用栗疫菌Cp-毒素处理板栗愈伤组织,分析了毒素对不同抗性的板栗品种愈伤组织病程相关蛋白、植物保卫素及游离脯氨酸含量的影响。研究表明:在较低浓度毒素处理范围内(5～100 μg/mL),板栗愈伤组织内的游离脯氨酸含量、植物保卫素含量、几丁质酶和β-1,3-葡聚糖酶活性与Cp-毒素有关,抗病品种酶活性升幅大于感病品种; 当毒素质量浓度高于100 μg/mL后,植物保卫素含量以及几丁质酶和β-1,3-葡聚糖酶活性下降,感病品种降幅大于抗病品种,而游离脯氨酸含量在品种间变化不大。说明在毒素低浓度处理下不同品种抗性物质含量都有所增加,证明了几丁质酶和β-1,3-葡聚糖酶、植物保卫素是板栗愈伤组织抵抗病原菌毒素的内在机制,而游离脯氨酸含量变化不是引起品种抗性差异的重要因子。

Abstract

In order to clarify the resistance mechanism of different chestnut cultivars to Cp-toxin stress, effects of different concentrations of Cp-toxin from Cryphonectria parasitica on the activities of chitinase, β-1,3-glucanase, the contents of phytoalexin, free proline(Pro)of chestnut callus were analyzed.In this study, a significant correlation was observed between the treatment concentration and chestnut resistance. The results showed that the activities of enzymes increased notably when treated with the low concentrations of Cp-toxin(5-100 μg/mL). When treated with the high concentrations of Cp-toxin(>100 μg/mL),the activities of enzymes and the contents of phytoalexin decreased notably.These data suggested that resistant materials in different varieties increased significantly under the treatment with low concentration of Cp-toxin. This study also indicates that the chitinase, β-1,3-glucanase and phytoalexins were the inherent mechanism for resistance of chestnut callus to toxin stress, and change of free proline content was not an important factor related to the resistance.

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韩珊,朱天辉,谯天敏,李姝江,汪菊,张博阳. 板栗愈伤组织对栗疫菌Cp-毒素的抗性响应[J]. 南京林业大学学报（自然科学版）. 2015, 39(05): 1-6 https://doi.org/10.3969/j.issn.1000-2006.2015.05.001

HAN Shan, ZHU Tianhui, QIAO Tianmin, LI Shujiang, WANG Ju, ZHANG Boyang. Resistance responses of Castanea mollissima callus to Cp-toxin under Cryphonectria parasitica stress[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(05): 1-6 https://doi.org/10.3969/j.issn.1000-2006.2015.05.001

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基金

收稿日期:2013-11-18 修回日期:2015-02-01
基金项目:高等学校博士学科点专项科研基金项目(201300329701); 国家自然科学基金项目(31400547)
第一作者:韩珊,副教授。*通信作者:朱天辉,教授。E-mail: hanshan6618@gmail.com。
引文格式:韩珊,朱天辉,谯天敏,等. 板栗愈伤组织对栗疫菌Cp-毒素的抗性响应[J]. 南京林业大学学报:自然科学版,2015,39(5):1-6.