比拉底白刺苗期抗旱性分析

鲁路; 张景波; 陈金慧; 周艳威; 成铁龙

doi:10.3969/j.issn.1000-2006.2015.05.010

比拉底白刺苗期抗旱性分析

鲁路,张景波,陈金慧,周艳威,成铁龙

南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (06) : 51-55.

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南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (06) : 51-55. DOI: 10.3969/j.issn.1000-2006.2015.05.010

研究论文

比拉底白刺苗期抗旱性分析

鲁路¹,张景波²,陈金慧¹,周艳威¹,成铁龙^1*

作者信息 +

Drought resistance analysis of Nitraria billardieri seedlings

LU Lu¹, ZHANG Jingbo², CHEN Jinhui¹, ZHOU Yanwei¹, CHENG Tielong^1*

Author information +

文章历史 +

摘要

为研究澳大利亚荒漠植物比拉底白刺的抗旱性,以比拉底白刺幼苗作为研究材料,在100 mmol/L甘露醇溶液模拟干旱的环境下,观察了植物形态变化,测定了植物的相对含水量(relative water content,RWC)、过氧化物酶活性(peroxidase,POD)、丙二醛(malondiadehyde,MDA)以及游离脯氨酸(proline,Pro)含量的动态变化。结果表明,干旱处理过程中,比拉底白刺叶片从基部往上逐渐萎蔫变黄,推测其通过保护顶芽的生命力以适应干旱环境; 相对含水量前期相对稳定,后逐渐下降; POD活性先缓慢升高,后快速上升; 游离Pro浓度总体呈上升趋势; MDA含量先升后降。差异显著性分析表明,与对照相比,POD和游离Pro的变化趋势与对照组相比差异显著,说明二者与比拉底白刺的抗旱性具有相关性,通过减少质膜过氧化保护细胞完整性; MDA含量变化不明显,可能由于POD和游离脯氨酸的作用使得白刺细胞的膜脂系统未遭受活性氧自由基的强烈攻击。

Abstract

The drought resistance research of Australia desert plants Nitraria billardieri was focused by observing morphological changes and analyzing variation trends of relative water content(RWC), enzymatic activity of peroxidase(POD), malondiadehyde(MDA), and proline(Pro)under drought stress simulated by 100 mmol/L mannitol solution. The results showed that the leaves turned yellow under drought stress and withered gradually from base to top, presumably by protecting the vitality of buds to adapt the arid environments. The relative water content kept relatively stable at early stage and then decreased gradually. The POD activity increased slowly at first and then increased rapidly. Free Pro concentration had been rising during the treatment. MDA content increased at first and then decreased. According to the statistical analysis, both POD and free Pro increased obviously compared with control group. They reduced plasma membrane peroxidation and kept the cell integrity which showed drought resistance of Nitraria billardieri. MDA concentration did not change significantly, this indicated that cytoplasmic membrane system was protected and did not seriously suffer from reactive oxygen free radical attacking under drought stress probably caused by increased concentration of POD and free Pro.

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鲁路,张景波,陈金慧,周艳威,成铁龙. 比拉底白刺苗期抗旱性分析[J]. 南京林业大学学报（自然科学版）. 2015, 39(06): 51-55 https://doi.org/10.3969/j.issn.1000-2006.2015.05.010

LU Lu, ZHANG Jingbo, CHEN Jinhui, ZHOU Yanwei, CHENG Tielong. Drought resistance analysis of Nitraria billardieri seedlings[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(06): 51-55 https://doi.org/10.3969/j.issn.1000-2006.2015.05.010

中图分类号： S718 Q945.78

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基金

收稿日期:2014-06-01 修回日期:2014-09-11
基金项目:国家自然科学基金项目(31270707); 中国林业科学研究院基本科研业务费专项项目(CAFYBB2011001); 国家林业局“948”引进项目(2011-4-27)
第一作者:鲁路,硕士生。*通信作者:成铁龙,高级工程师。E-mail:ctielong@126.com。
引文格式:鲁路,张景波,陈金慧,等. 比拉底白刺苗期抗旱性分析[J]. 南京林业大学学报:自然科学版,2015,39(6):51-55.