DNA条形码试剂盒检测技术在墨天牛鉴定中的应用

陈梦义; 徐梅; 钱路; 安榆林; 郝德君

doi:10.3969/j.issn.1000-2006.2015.06.002

陈梦义,徐梅,钱路,安榆林,郝德君

南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (06) : 7-11.

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南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (06) : 7-11. DOI: 10.3969/j.issn.1000-2006.2015.06.002

专题报道

DNA条形码试剂盒检测技术在墨天牛鉴定中的应用

陈梦义^1,2,徐梅³,钱路³,安榆林³,郝德君^1,2*

作者信息 +

The application of DNA barcoding identification kit technology to Monochamus Dejean

CHEN Mengyi^1,2, XU Mei³, QIAN Lu³, AN Yulin³, HAO Dejun^1,2*

Author information +

文章历史 +

摘要

为了对进境口岸截获的俄罗斯木材中的墨天牛属(非中国种)害虫进行准确、快速鉴定,笔者利用DNA 条码试剂盒检测技术,基于墨天牛线粒体COⅠ(线粒体细胞色素氧化酶亚基I)基因设计一对巢式引物,对截获墨天牛属中的8 个种进行COⅠ扩增,将扩增序列上传至有害生物信息网站,依据碱基位点在不同种间的排列和组成的差异,获得不同种在相应位点上的特有碱基,即标记性碱基,以此作为区分墨天牛种的依据。通过这种墨天牛属DNA 条码试剂盒检测技术,可为口岸检疫鉴定提供有效的技术手段。

Abstract

In order to rapidly and accurately identify the Monochamus of wood from Russian, we employed DNA barcode detection kit technology and designed a pair of nested primers based on mitochondrial COⅠ gene(mitochondrial cytochrome oxidase subunit I). Furthermore, COⅠ gene sequence of eight Monocharnus species was amplificated, and uploaded to pest information site. According to differences of arrangement and composition among these species at base sites, species unique bases was got in the corresponding sites, which is named specific site to distinguish this species. In this way, we developed the DNA barcoding identification kit technology to Monochamus species, which will provide a new way for port quarantine identification.

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陈梦义,徐梅,钱路,安榆林,郝德君. DNA条形码试剂盒检测技术在墨天牛鉴定中的应用[J]. 南京林业大学学报（自然科学版）. 2015, 39(06): 7-11 https://doi.org/10.3969/j.issn.1000-2006.2015.06.002

CHEN Mengyi, XU Mei, QIAN Lu, AN Yulin, HAO Dejun. The application of DNA barcoding identification kit technology to Monochamus Dejean[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(06): 7-11 https://doi.org/10.3969/j.issn.1000-2006.2015.06.002

中图分类号： S763

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基金

收稿日期:2014-12-10 修回日期:2015-09-09
基金项目:“十二五”国家科技支撑计划(2012BAK11B03); 国家自然科学基金项目(31170606); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:陈梦义,硕士生。*通信作者:郝德君,教授。E-mail: dejunhao@163.com。
引文格式:陈梦义,徐梅,钱路,等. DNA条形码试剂盒检测技术在墨天牛鉴定中的应用[J]. 南京林业大学学报:自然科学版,2015,39(6):7-11.