天女木兰是集芳香、药用、食用和观赏于一体的珍稀濒危植物,呈“间断式”分布。为探知天女木兰种下分类及其亲缘关系,利用扫描电镜对7个分布地天女木兰叶表皮微形态特征指标进行定性描述及主成分分析和聚类分析。结果表明:①表皮细胞分为浅波状和深波状两种类型; 气孔器为椭圆形,分为凸起和凹陷两种; 叶表结晶体有圆球形和长柱形; 表皮毛密,分为单细胞毛、单列双细胞毛和单列多细胞毛3种类型; 叶表皮气孔大小、密度和表皮毛长短及密度等均存在差异。②主成分分析选出3个主成分,方差累计贡献率达到87.394%。③聚类分析结果表明,7个分布地天女木兰种群分为两类。第Ⅰ类包括吉林石湖和河北祖山的天女木兰,表现为叶下表皮细胞呈浅波状,气孔密度小,气孔凸起,气孔长轴小于10 μm,叶表结晶体呈圆球形; 第Ⅱ类包括浙江清凉峰、安徽黄山、贵州雷公山、贵州麻江和广西猫儿山的天女木兰,表现为叶下表皮细胞多呈深波状,气孔密度大,气孔多凹陷,气孔长轴大于10 μm,叶表结晶体呈长柱形。这表明各天女木兰间的亲缘关系远近与地理分布相关,叶表皮微形态特征可作为种下分类和探讨天女木兰种群间亲缘关系的重要特征。
Abstract
Magnolia sieboldii is an endangered, rare woody plant species, with a discontinuous natural distribution and high values in perfume, pharmaceutical, food and ornamental industries. To explore genetic variations within-species and relationships among different habitats, we used scanning electronic microscope, principal component analysis, and cluster analysis to quantify the micromorphological characters of leaf epidermis among M. sieboldii populations from seven habitats. The results showed that there were two types of epidermal cells(shallow wavy and deep wavy), two types of stomatal apparatuses(elliptically convex and concave), two types of leaf epidermis crystals(spherical and cylindrical), three types of dense epidermal hairs(unicellular, single-row bicellular and single-row multicellular), as well as variations in stomata size, density and epidermal hair length and density. The principal component analysis identified three principal components accounted for 87.394% of the total variation in micromorphological characters of leaf epidermis. The cluster analysis showed that M. sieboldii from seven habitats belonged to two groups. The first group included the M.sieboldii from Shihu in Jilin Province and Zushan in Hebei Province, and this group had shallow wavy leaf epidermal cells, lower stomatal density, convex stomatal apparatus, short stomatal axis(<10 μm)and round leaf crystals. The second group included the M.sieboldii from Qingliangfeng in Zhejiang Province, Huangshan in Anhui Province, Leigong Mountain and Majiang in Guizhou Province and Maoershan in Guangxi Province. This group generally had deep wavy leaf epidermal cells, higher stomatal density, concave stomata, long stomatal axis(>10 μm)and long cylindrical leaf crystals. These results suggested that the genetic relationships among different habitat in M. sieboldii were related to the geographical distribution of the species and that the micromorphological characters of leaf epidermis could be used to study genetic variations and relationships among different habitat in M. sieboldii.
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参考文献
[1] 傅立国. 中国植物红皮书-稀有濒危植物[M]. 北京: 科学出版社, 1992: 736.
[2] 吴小巧, 黄宝龙, 丁雨龙. 中国珍稀濒危植物保护研究现状与进展[J]. 南京林业大学学报(自然科学版), 2004, 28(2): 72-76.Doi:10.3969/j.issn.1000-2006.02.015.
Wu X Q, Huang B L, Ding Y L. The advance on the study of protection of rare and endangered plants in China[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2004, 28(2): 72-76.
[3] 汪松, 解焱. 中国物种红色名录[M]. 北京: 高等教育出版社, 2004: 327.
[4] 宋连芳, 富玉, 秦丽. 建立天女木兰资源保护区的探讨[J]. 吉林林业科技, 2001, 30(2): 35-38.Doi:10.16115/j.cnki.issn.1005-7129.2001.02.013.
Song L F, Fu Y, Qin L. Discussion on establishment of Magnolia parviflora conservancy[J]. Jilin Forestry Science and Technology, 2001, 30(2): 35-38.
[5] 杜凤国, 王欢, 刘春强. 天女木兰群落物种多样性的研究[J]. 东北师范大学学报, 2006, 38(2): 91-94.Doi:10.16163/j.cnki.22-1123/n.2006.02.022.
Du F G, Wang H, Liu C Q. Study on the species diversity in Magnolia sieboldii community[J]. Journal of Northeast Normal University, 2006, 38(2): 91-94.
[6] 杜凤国, 王欢, 杨德冒, 等. 天女木兰种子形态及生物学特性[J]. 北华大学学报, 2006, 7(3): 269-272.
Du F G, Wang H, Yang D M, et al. Morphology and biological characters of seed of Magnolia sieboldii K.Koch[J]. Journal of Beihua University, 2006, 7(3): 269-272.
[7] 丁磊, 胡万良, 王伟, 等. 遮荫对天女木兰光合特性及生长的影响[J]. 林业资源管理, 2009(3): 61-65.Doi:10.13466/j.cnki.lyzygl.2009.03.016.
Ding L, Hu W L, Wang W, et al. Effects of shading on the photosynthetic characteristics and growth of Magnolia sieboldii[J]. Forest Resources Management, 2009(3): 61-65.
[8] 杜凤国, 姜洪源, 郭忠玲, 等. 吉林濒危植物天女木兰种群分布格局与生态位研究[J]. 南京林业大学学报(自然科学版), 2011, 35(3): 33-37.Doi:10.3969/j.issn.1000-2006.2011.03.007.
Du F G, Jiang H Y, Guo Z L, et al. Study on the distribution pattern and niche of endangered Magnolia sieboldii in Jilin province[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2011, 35(3): 33-37.
[9] 陆秀君, 李天来, 倪伟东. 天女木兰种子休眠特性的研究[J]. 沈阳农业大学学报, 2006, 37(5): 703-706.
Lu X J, Li T L, Ni W D. Study on the dormant characteristic of Magnolia sieboldii K.Koch seeds[J]. Journal of Shenyang Agricultural University, 2006, 37(5): 703-706.
[10] 陆秀君, 王妮妮, 李天来, 等. 不同浸种和催芽处理对天女木兰种子的催芽效果[J]. 西北农林科技大学学报, 2008, 36(5): 135-140.Doi:10.13207/j.cnki.jnwafu.2008.05.028.
Lu X J, Wang N N, Li T L, et al. Effect of different soaking and accelerating germination disposals on forced germination of Magnolia sieboldii K.Koch seeds[J]. Journal of Northwest A&F University, 2008, 36(5): 135-140.
[11] 马吉龙, 李艳君. 天女花种子繁殖及其在园林中的应用栽培[J]. 河北林果研究, 1999, 14(3): 238-241.
Ma J L, Li Y J. Application and cultivation of Magnolia sieboldii in gardens and its propagation by seed[J]. Hebei Journal of Forestry and Orchard Research, 1999, 14(3): 238-241.
[12] 王欢, 杜凤国, 杨德冒, 等. 天女木兰硬枝扦插繁殖初步研究[J]. 北华大学学报, 2005, 6(4): 352-354.
Wang H, Du F G, Yang D M, et al. Preliminary study on the cutting propagation of firmwood branches of Magnolia sieboldii[J]. Journal of Beihua University, 2005, 6(4): 352-354.
[13] 王欢, 杜悦, 王子明, 等. 天女木兰嫩枝扦插繁殖技术[J]. 林业科技, 2013, 38(4): 45-47.
Wang H, Du Y, Wang Z M, et al. Propagation techniques of softwood cutting of Magnolia sieboldii[J]. Forestry Science & Technology, 2013, 38(4): 45-47.
[14] 杜凤国, 刁绍起, 王欢, 等. 天女木兰的组织培养[J]. 东北林业大学学报, 2006, 34(2): 42-43.Doi:10.13759/j.cnki.dlxb.2006.02.015.
Du F G, Diao S Q, Wang H, et al. Tissue culture of Magnolia sieboldii K. Koch[J]. Journal of Northeast Forestry University, 2006, 34(2): 42-43.
[15] Ueda K. Taxonomic study of Magnolia sieboldii K. Koch[J]. Acta Phytotax Geobot, 1980, 31: 117-125.
[16] Kikuchi S, Isagi Y. Microsatellite genetic variation in small and isolated populations of Magnolia sieboldii ssp. japonica[J]. Heredity, 2002, 88(4): 313-321.
[17] 刘玉宗, 王志杰. 祖山林区天女花资源及其开发利用[J]. 林业科技开发, 2001, 15(S): 60.
Liu Y Z, Wang Z J. The exploitation and utilization of Magnolia sieboldii resources[J]. The Forestry Science and Technology Development, 2001, 15(S): 60.
[18] 刘大斌. 天女木兰的形态特征及开发利用[J]. 现代园艺, 2014(7): 65.Doi:10.14051/j.cnki.xdyy.2014.14.157.
Liu D B. Morphological characteristics and the development and utilization of Magnolia sieboldii K. Koch[J]. Modern Gardening, 2014(7): 65.
[19] 田建平, 胡远艳, 张俊清, 等. 9种梧桐科植物叶表皮特征的扫描电镜研究[J]. 西北植物学报, 2008, 28(7): 1326-1332.
Tian J P, Hu Y Y, Zhang J Q, et al. Characteristics of leaf epidermis among nine species in Sterculiaceae by SEM[J]. Journal of Northwest Plants, 2008, 28(7): 1326-1332.
[20] 田丽, 周新宇, 孙航, 等. 7种委陵菜属植物叶表皮微形态的研究[J]. 草业科学, 2009, 26(2): 53-56.
Tian L, Zhou X Y, Sun H, et al. The leaf epidermal micro-morphology of the seven species of Potentilla[J]. Pratacultural Science, 2009, 26(2): 53-56.
[21] 马艳, 郭庆梅, 周凤琴. 蒙药鹅绒藤扫描电镜的观察研究[J]. 电子显微学报, 2011, 30(3): 253-256.
Ma Y, Guo Q M, Zhou F Q. Study on the Mongolian medicine above-ground of Cynanchum chinense R. Br. by scanning electron microscopic technique[J]. Journal of Electron Microscopy, 2011, 30(3): 253-256.
[22] 罗玉兰, 张国兵, 崔心红, 等. 鹅掌楸属植物叶表皮扫描电镜观察[J]. 中国农学通报, 2012, 28(34): 40-45.
Luo Y L, Zhang G B, Cui X H, et al. Observation of leaves of Liriodendron by scanning electron microscope[J]. Chinese Agricultural Science Bulletin, 2012, 28(34): 40-45.
[23] 卢纹岱. Spss for Windows统计分析[M]. 北京: 电子工业出版社, 2002: 201-213.
[24] 张淑媛, 杜凤国, 王瑞俭, 等. 天女木兰叶片形态特征的数值分析[J]. 北华大学学报, 2014, 15(2): 240-242.
Zhang S Y, Du F G, Wang R J, et al. Numerical analysis on leaf morphology of Magnolia sieboldii[J]. Journal of Beihua University, 2014, 15(2): 240-242.
基金
收稿日期:2015-07-01 修回日期:2015-12-01
基金项目:国家林业公益性行业科研专项项目(201004095)
第一作者:张淑媛(1184568667@qq.com)。*通信作者:杜凤国(dfg4656@qq.com),教授。
引文格式:张淑媛,杜凤国, 王瑞俭,等. 不同分布地天女木兰叶表皮微形态研究[J]. 南京林业大学学报(自然科学版),2016,40(4):56-62.
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