瓦雷兹芽孢杆菌FZB42菌株拮抗两种树木病原真菌及其机理研究

doi:10.3969/j.issn.1000-2006.2016.06.016

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南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (06): 103-108.doi: 10.3969/j.issn.1000-2006.2016.06.016

瓦雷兹芽孢杆菌FZB42菌株拮抗两种树木病原真菌及其机理研究

樊奔,李昱龙,祁奇

南方现代林业协同创新中心,南京林业大学林学院,江苏南京 210037

出版日期:2016-12-18 发布日期:2016-12-18
基金资助:
基金项目:国家自然科学基金项目(31100081); 江苏省自然科学基金项目(BK20151514); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:樊奔(fanben@njfu.edu.cn),副教授。
引文格式:樊奔,李昱龙,祁奇. 瓦雷兹芽孢杆菌FZB42菌株拮抗两种树木病原真菌及其机理研究[J]. 南京林业大学学报(自然科学版),2016,40(6):103-108.

Study on antagonism and mechanisms of Bacillus velezensis FZB42 strain against two species of tree fungi pathogens

FAN Ben, LI Yulong, QI Qi

Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Online:2016-12-18 Published:2016-12-18

摘要/Abstract

摘要： 镰刀菌和交链孢菌是导致树木病害的两种常见病原真菌,常导致苗圃植物严重的危害和经济损失。瓦雷兹芽孢杆菌FZB42菌株具有高效生防性能和植物促生活性,为进一步探明该菌株的生防机理,首先测试了FZB42对镰刀菌和交链孢菌的抑制效果。在了解该菌株抑制作用的基础上,进一步构建质粒,通过同源重组的方式构建了FZB42的3个抗生素合成缺损菌株(ΔbmyA,ΔfenA、ΔbmyAΔfenA)。对这3个突变株进一步测试表明,FZB42合成的物质bacillomycin D和fengycin是抑制两种真菌生长的重要原因,且这两种物质的拮抗作用具有明显的协同强化效果。

Abstract: Fusarium and Alternaria are two kinds of common pathogenic fungi which can cause tree diseases, especially harmful to nursery plants, and may lead to serious economic loss. Biological control is a promising and environmentally friendly method to control the phytopathogens. Bacillus velezensis FZB42 strain is a plant growth promoting rhizobacterium with potent biocontrol activity. To explore the spectrum of its antagonistic activity, we tested the effect of FZB42 on two fungi species, Fusarium solani and Alternaria tenuis. The results showed that FZB42 could inhibit the growth of both of the species. Further, we constructed three FZB42 mutants(ΔbmyA, ΔfenA and ΔbmyAΔfenA)via homologous recombination. Using the three mutants we demonstrated that bacillomycin D and fengycin synthesized by FZB42 are important to inhibit the growth of the fungi, and the two antagonistic molecules have significant synergistic effect.

中图分类号:

S763
Q754

樊奔,李昱龙,祁奇. 瓦雷兹芽孢杆菌FZB42菌株拮抗两种树木病原真菌及其机理研究[J]. 南京林业大学学报（自然科学版）, 2016, 40(06): 103-108.

FAN Ben, LI Yulong, QI Qi. Study on antagonism and mechanisms of Bacillus velezensis FZB42 strain against two species of tree fungi pathogens[J].Journal of Nanjing Forestry University (Natural Science Edition), 2016, 40(06): 103-108.DOI: 10.3969/j.issn.1000-2006.2016.06.016.

参考文献

[1] 朱天辉.苗圃植物病虫害防治[M].北京: 中国林业出版社,2002:1-13.
[2] Lacey L A, Goettel M S. Current developments in microbial control of insect pests and prospects for the early 21st Century[J]. Entomophaga, 1995, 40(1): 3-27. Doi:10.1007/bf02372677.
[3] Schmitt C B, Burgess N D, Coad L, et al. Global analysis of the protection status of the world's forests[J].Biological Conservation, 2009, 142(10): 2122-2130. Doi:10.1016/j.biocon.2009.04.012.
[4] Borriss R, Chen X H, Rueckert C, et al. Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7^T and FZB42^T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons[J]. International Journal of Systematic and Evolutionary Microbiology, 2010, 61(8): 1786-1801. Doi:10.1099/ijs.0.023267-0.
[5] Dunlap C A, Kim S J, Kwon S W, et al. Bacillus velezensis is not a later heterotypic synonym of Bacillus amyloliquefaciens; Bacillus methylotrophicus, Bacillus amyloliquefaciens subsp. plantarum and ‘Bacillus oryzicola' are later heterotypic synonyms of Bacillus velezensis based on phylogenomics [J]. Int J Syst Evol Microbiol, 2015,24(10):000858.
[6] Chen X H, Koumoutsi A, Scholz R, et al. Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42[J]. Nat Biotechnol, 2007, 25(9): 1007-1014. Doi:10.1038/nbt1325.
[7] Fan B, Chen X H, Budiharjo A, et al. Efficient colonization of plant roots by the plant growth promoting bacterium Bacillus amyloliquefaciens FZB42, engineered to express green fluorescent protein[J]. J Biotechnol, 2011, 151(4):303-311. Doi:10.1016/j.jbiotec.2010.12.022.
[8] Koumoutsi A, Chen X H, Vater J, et al. DegU and YczE positively regulate the synthesis of bacillomycin D by Bacillus amyloliquefaciens strain FZB42[J]. Appl Environ Microbiol, 2007, 73(21): 6953-6964. Doi:10.1128/AEM.00565-07.
[9] Fan B, Carvalhais L C, Becker A, et al. Transcriptomic profiling of Bacillus amyloliquefaciens FZB42 in response to maize root exudates[J]. BMC Microbiol, 2012, 12: 116. Doi:10.1186/1471-2180-12-116.
[10] Fan B, Li L, Chao Y, et al. dRNA-Seq reveals Genomewide TSSs and noncoding RNAs of plant beneficial rhizobacterium Bacillus amyloliquefaciens FZB42 [J]. PLoS One, 2015, 10(11):e0142002. Doi:10.1371/journal.pone.0142002.
[11] Chen X H, Scholz R, Borriss M, et al. Difficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens are efficient in controlling fire blight disease[J]. Journal of Biotechnology, 2009, 140(1): 38-44. Doi:10.1016/j.jbiotec.2008.10.015.
[12] Budiharjo A, Chowdhury S P, Dietel K, et al. Transposon mutagenesis of the plant-associated Bacillus amyloliquefaciens ssp. plantarum FZB42 revealed that the nfrA and RBAM17410 genes are involved in plant-microbe-interactions[J]. PLoS One, 2014, 9(5): e98267. Doi:10.1371/journal.pone.0098267.
[13] Chen X H, Koumoutsi A, Scholz R, et al. More than anticipated-production of antibiotics and other secondary metabolites by Bacillus amyloliquefaciens FZB42[J]. J Mol Microbiol Biotechnol, 2009, 16(1-2): 14-24. Doi:10.1159/000142891.
[14] Schneider K, Chen X H, Vater J, et al. Macrolactin is the polyketide biosynthesis product of the pks2 cluster of Bacillus amyloliquefaciens FZB42[J]. J Nat Prod, 2007, 70(9): 1417-1423. Doi:10.1021/np070070k.
[15] Koumoutsi A, Chen X H, Henne A, et al. Structural and functional characterization of gene clusters directing nonribosomal synthesis of bioactive cyclic lipopeptides in Bacillus amyloliquefaciens strain FZB42[J]. J Bacteriol, 2004, 186(4): 1084-1096. Doi:10.1128/jb.186.4.1084-1096.2004.
[16] Kalyon B, Helaly S E, Scholz R, et al. Plantazolicin A and B: structure elucidation of ribosomally synthesized thiazole/oxazole peptides from Bacillus amyloliquefaciens FZB42 [J]. Organic Letters, 2011, 13(12): 2996-2999. Doi:10.1021/ol200809m.
[17] Scholz R, Vater J, Budiharjo A, et al. Amylocyclicin, a novel circular bacteriocin produced by Bacillus amyloliquefaciens FZB42 [J]. Journal of Bacteriology, 2014, 196(10):1842-1852. Doi:10.1128/JB.01474-14.
[18] Ogata H, Cao Z, Losi A, et al. Crystallization and preliminary X-ray analysis of the LOV domain of the blue-light receptor YtvA from Bacillus amyloliquefaciens FZB42[J]. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2009, 65(Pt 8): 853-855. Doi:10.1107/S1744309109026670.
[19] Wu L, Wu H, Chen L, et al. Difficidin and bacilysin from Bacillus amyloliquefaciens FZB42 have antibacterial activity against Xanthomonas oryzae rice pathogens [J]. Sci Rep, 2015, 5:12975. Doi:10.1038/srep12975.
[20] Liu Z, Budiharjo A, Wang P, et al. The highly modified microcin peptide plantazolicin is associated with nematicidal activity of Bacillus amyloliquefaciens FZB42[J]. Appl Microbiol Biotechnol, 2013, 97(23):10081-10090. Doi:10.1007/s00253-013-5247-5.
[21] Moyne A L, Shelby R, Cleveland T E, et al. Bacillomycin D: an iturin with antifungal activity against Aspergillus flavus [J]. J Appl Microbiol, 2001, 90(4):622-629. Doi:10.1046/j.1365-2672.2001.01290.x.
[22] Peypoux F, Besson F, Michel G, et al. Structure of bacillomycin D, a new antibiotic of the iturin group [J]. Eur J Biochem, 1981, 118(2):323-327.
[23] Vanittanakom N, Loeffler W, Koch U, et al. Fengycin—a novel antifungal lipopeptide antibiotic produced by Bacillus subtilis F-29-3[J]. J Antibiot, 1986, 39(7): 888-901.
[24] Tao Y, Bie X M, Lv F X, et al. Antifungal activity and mechanism of fengycin in the presence and absence of commercial surfactin against Rhizopus stolonifer [J]. The Journal of Microbiology, 2011, 49(1):146-150. Doi:10.1007/s12275-011-0171-9.
[25] Guo Q, Dong W, Li S, et al. Fengycin produced by Bacillus subtilis NCD-2 plays a major role in biocontrol of cotton seedling damping-off disease [J]. Microbiological Research, 2014, 169(7-8):533-540.Doi:10.1016/j.micres.2013.12.001.

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瓦雷兹芽孢杆菌FZB42菌株拮抗两种树木病原真菌及其机理研究

Study on antagonism and mechanisms of Bacillus velezensis FZB42 strain against two species of tree fungi pathogens

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