两株解磷青霉对马尾松根际土壤的微生态效应及其促生作用

乔欢; 吴小芹; 魏伟

doi:10.3969/j.issn.1000-2006.2016.06.017

乔欢,吴小芹,魏伟

南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (06) : 109-116.

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南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (06) : 109-116. DOI: 10.3969/j.issn.1000-2006.2016.06.017

研究论文

两株解磷青霉对马尾松根际土壤的微生态效应及其促生作用

乔欢,吴小芹^*,魏伟

作者信息 +

Effects of two phosphate-solubilizing Penicillium strains on microbial community structure and their growth-promoting in masson pine rhizosphere

QIAO Huan, WU Xiaoqin^*, WEI Wei

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摘要

为了研究从马尾松根际土壤中筛选出的2株高效解磷真菌(瓜纳卡斯特青霉JP-NJ2和嗜松青霉JP-NJ4)对马尾松根际土壤微生物种群数量及松树生长的影响。该研究通过Biolog微平板法探究该两株解磷青霉对马尾松根际土壤微生物群落结构的影响,同时观测其对马尾松的持续促生效果,并对马尾松根际土壤产生的微生态效应进行初步分析。结果表明:冬夏两季,2种解磷真菌及其代谢产物均对土壤中微生物物种优势度及丰富度产生了显著影响; 相对于对照处理,解磷真菌处理的根际土壤中细菌数量显著提高,夏季时最高,为59.27×10⁴ cfu/g; 瓜纳卡斯特青霉JP-NJ2及嗜松青霉JP-NJ4菌悬液处理的根际土壤,夏季时物种丰富度指数分别为2.835 0与3.120 0,均低于对照处理,说明夏季时解磷青霉降低了土壤物种丰富度。两株解磷青霉接种600 d后对马尾松仍具有良好的促生作用,苗高增长率可达88.7%,地径增长率最高为53.5%。解磷青霉的施用,明显改变了根际微生物群落中细菌、真菌及放线菌数量,微生物总量也随之变化; 两株供试青霉均对马尾松具有良好的持续促生作用,其中瓜纳卡斯特青霉JP-NJ2菌悬液的促生效果最佳。

Abstract

Two fungal strains Penicillium guanacastense JP-NJ2 and P. pinophilum JP-NJ4 which had strong capability of dissolving phosphate was isolated from Pinus massoniana rhizosphere early. The effects of the strains on microbial community structure and growth-promoting in masson pine rhizosphere were studied. Microbial community structure in masson pine rhizosphere and growth-promoting of the pines were measured with the Biolog method and then the effects of two phosphate-solubilizing fungi(PSF)were analyzed. The impact of two strains on growth of masson pine was observed. The applications of PSF P. guanacastense JP-NJ2 and P. pinophilum JP-NJ4 and their metabolite significantly changed the species dominance and richness index both in winter and summer. The two PSF raised the number of fungi in the soil and reached the largest biomass in summer(59.27×10⁴ cfu/g). In summer, species richness indexes treated by the JP-NJ2 suspension and JP-NJ4 suspension were 2.835 0 and 3.120 0, respectively. Both of the species richness indexes of two fungal suspensions were lower than that of the control. This indicated that soil species richness reduced in summer. The application of JP-NJ2 and JP-NJ4 in masson pine rhizosphere significantly promoted pine growth after inoculation 600 days. The height of masson pine seedlings treated with fungal suspension increased 88.7% and root crown diameter incremented 53.5%.The applications of P. guanacastense JP-NJ2 and P. pinophilum JP-NJ4 were found greatly to alter the number of bacteria, fungi and actinomycetes as well as the total number of microorganisms. Both of two strains could promote masson pine growth well and the effect of P. guanacastense JP-NJ2 suspension was the best. The results of this research showed an important indicative reference to develop phosphate-solubilizing microbiological fertilizer.

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乔欢,吴小芹,魏伟. 两株解磷青霉对马尾松根际土壤的微生态效应及其促生作用[J]. 南京林业大学学报（自然科学版）. 2016, 40(06): 109-116 https://doi.org/10.3969/j.issn.1000-2006.2016.06.017

QIAO Huan, WU Xiaoqin, WEI Wei. Effects of two phosphate-solubilizing Penicillium strains on microbial community structure and their growth-promoting in masson pine rhizosphere[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2016, 40(06): 109-116 https://doi.org/10.3969/j.issn.1000-2006.2016.06.017

中图分类号： S718.5

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基金

基金项目:“十二五”国家科技支撑计划(2012BAD19B0703); 国家林业公益性行业科研专项项目(201004061); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:乔欢(qh_qiaohuan@163.com)
*通信作者:吴小芹(xqwu@njfu.edu.cn),教授。
引文格式:乔欢,吴小芹,魏伟. 两株解磷青霉对马尾松根际土壤的微生态效应及其促生作用[J]. 南京林业大学学报(自然科学版),2016,40(6):109-116.