两株解磷青霉对马尾松根际土壤的微生态效应及其促生作用

doi:10.3969/j.issn.1000-2006.2016.06.017

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南京林业大学学报（自然科学版） ›› 2016, Vol. 59 ›› Issue (06): 109-116.doi: 10.3969/j.issn.1000-2006.2016.06.017

两株解磷青霉对马尾松根际土壤的微生态效应及其促生作用

乔欢,吴小芹^*,魏伟

南方现代林业协同创新中心,南京林业大学林学院,江苏南京 210037

出版日期:2016-12-18 发布日期:2016-12-18
基金资助:
基金项目:“十二五”国家科技支撑计划(2012BAD19B0703); 国家林业公益性行业科研专项项目(201004061); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:乔欢(qh_qiaohuan@163.com)
*通信作者:吴小芹(xqwu@njfu.edu.cn),教授。
引文格式:乔欢,吴小芹,魏伟. 两株解磷青霉对马尾松根际土壤的微生态效应及其促生作用[J]. 南京林业大学学报(自然科学版),2016,40(6):109-116.

Effects of two phosphate-solubilizing Penicillium strains on microbial community structure and their growth-promoting in masson pine rhizosphere

QIAO Huan, WU Xiaoqin^*, WEI Wei

Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Online:2016-12-18 Published:2016-12-18

摘要/Abstract

摘要： 为了研究从马尾松根际土壤中筛选出的2株高效解磷真菌(瓜纳卡斯特青霉JP-NJ2和嗜松青霉JP-NJ4)对马尾松根际土壤微生物种群数量及松树生长的影响。该研究通过Biolog微平板法探究该两株解磷青霉对马尾松根际土壤微生物群落结构的影响,同时观测其对马尾松的持续促生效果,并对马尾松根际土壤产生的微生态效应进行初步分析。结果表明:冬夏两季,2种解磷真菌及其代谢产物均对土壤中微生物物种优势度及丰富度产生了显著影响; 相对于对照处理,解磷真菌处理的根际土壤中细菌数量显著提高,夏季时最高,为59.27×10⁴ cfu/g; 瓜纳卡斯特青霉JP-NJ2及嗜松青霉JP-NJ4菌悬液处理的根际土壤,夏季时物种丰富度指数分别为2.835 0与3.120 0,均低于对照处理,说明夏季时解磷青霉降低了土壤物种丰富度。两株解磷青霉接种600 d后对马尾松仍具有良好的促生作用,苗高增长率可达88.7%,地径增长率最高为53.5%。解磷青霉的施用,明显改变了根际微生物群落中细菌、真菌及放线菌数量,微生物总量也随之变化; 两株供试青霉均对马尾松具有良好的持续促生作用,其中瓜纳卡斯特青霉JP-NJ2菌悬液的促生效果最佳。

Abstract: Two fungal strains Penicillium guanacastense JP-NJ2 and P. pinophilum JP-NJ4 which had strong capability of dissolving phosphate was isolated from Pinus massoniana rhizosphere early. The effects of the strains on microbial community structure and growth-promoting in masson pine rhizosphere were studied. Microbial community structure in masson pine rhizosphere and growth-promoting of the pines were measured with the Biolog method and then the effects of two phosphate-solubilizing fungi(PSF)were analyzed. The impact of two strains on growth of masson pine was observed. The applications of PSF P. guanacastense JP-NJ2 and P. pinophilum JP-NJ4 and their metabolite significantly changed the species dominance and richness index both in winter and summer. The two PSF raised the number of fungi in the soil and reached the largest biomass in summer(59.27×10⁴ cfu/g). In summer, species richness indexes treated by the JP-NJ2 suspension and JP-NJ4 suspension were 2.835 0 and 3.120 0, respectively. Both of the species richness indexes of two fungal suspensions were lower than that of the control. This indicated that soil species richness reduced in summer. The application of JP-NJ2 and JP-NJ4 in masson pine rhizosphere significantly promoted pine growth after inoculation 600 days. The height of masson pine seedlings treated with fungal suspension increased 88.7% and root crown diameter incremented 53.5%.The applications of P. guanacastense JP-NJ2 and P. pinophilum JP-NJ4 were found greatly to alter the number of bacteria, fungi and actinomycetes as well as the total number of microorganisms. Both of two strains could promote masson pine growth well and the effect of P. guanacastense JP-NJ2 suspension was the best. The results of this research showed an important indicative reference to develop phosphate-solubilizing microbiological fertilizer.

中图分类号:

S718.5

乔欢,吴小芹,魏伟. 两株解磷青霉对马尾松根际土壤的微生态效应及其促生作用[J]. 南京林业大学学报（自然科学版）, 2016, 59(06): 109-116.

QIAO Huan, WU Xiaoqin, WEI Wei. Effects of two phosphate-solubilizing Penicillium strains on microbial community structure and their growth-promoting in masson pine rhizosphere[J].Journal of Nanjing Forestry University (Natural Science Edition), 2016, 59(06): 109-116.DOI: 10.3969/j.issn.1000-2006.2016.06.017.

参考文献

[1] 刘文干. 三株红壤高效溶磷菌的分离、鉴定、溶磷特性及对花生促生效应的研究[D]. 南京: 南京农业大学, 2012. Liu W G. Study on screening, identification, characteristic of phosphate solubilizing and promotion effect of three phosphate solubilizingmicroorganism from red soil[D]. Nanjing: Nanjing Agricultural University, 2012.
[2] 吴才武, 赵兰坡. 土壤微生物多样性的研究方法[J]. 中国农学通报, 2011, 27(11): 231-235. Wu C W, Zhao L P. Technologies on soil microbiology diversity[J]. Chinese Agricultural Science Bulletin, 2011, 27(11): 231-235.
[3] 冯月红, 姚拓, 龙瑞军. 土壤解磷菌研究进展[J]. 草原与草坪, 2003, 23(1): 3-7.Doi:10.3969/j.issn.1009-5500.2003.01.001. Feng Y H, Yao T, Long R J.Research progress of phosphate-dissolving microorganisms in plant rhizosphere[J]. Grassland and Turf, 2003, 23(1): 3-7.
[4] 金术超, 杜春梅, 平文祥, 等. 解磷微生物的研究进展[J]. 微生物学杂志, 2006, 26(2): 73-78. Jin S C, Du C M, Ping W X, et al. Advance in phosphorus-dissolving microbes[J]. Journal of Microbiology, 2006, 26(2): 73-78.
[5] 骆宗诗, 温佐吾. 马尾松次生林研究现状和展望[J]. 贵州林业科技, 2004, 32(3): 47-55. Luo Z S, Wen Z W.The status and prospects in research of secondary masson pine forest[J]. Guizhou Forestry Science and Technology, 2004, 32(3): 47-55.
[6] 黄鹏飞,刘君昂,靳爱仙, 等. 马尾松根际土壤溶磷菌分离筛选、鉴定及其溶磷效果研究[J]. 中国农学通报, 2012, 28(19): 12-16.Doi:10.3969/j.issn.1000-6850.2012.19.003. Huang P F, Liu J A, Jin A X, et al. Isolation and Screening, Identification of phosphorus-solubilizing bacteria in rhizosphere soil of Pinus massoniana and its phosphate-degradation capacity[J]. Chinese Agricultural Science Bulletin, 2012, 28(19): 12-16.
[7] 吴庆梅, 黄宝灵, 吕成群, 等. 不同松林根际促生菌的数量研究[J]. 广西农业科学, 2009, 40(5): 521-526.Doi:10.3969/j.issn.2095-119/2009.05.018. Wu Q M, Huang B L, Lv C Q, et al. Population of growth-promoting rhizobacteria(GPR)in different pine forests[J]. Guangxi Agricultural Sciences, 2009, 40(5): 521-526.
[8] Sylvia D M, Fuhmann J J. Princiles and applications of soil microbiology[J]. New Age International, 2005,31(2):11-68.
[9] Peix A, Rivas-Boyero A A, Mateos P F, et al. Growth promotion of chickpea and barley by a phosphate solubilizing strain of Mesorhizobium mediterraneum under growth chamber conditions[J]. Soil Biology and Biochemistry, 2001, 33(1): 103-110.Doi:10.1016/S0038-0717(00)00120-6.
[10] Zaidi A, Khan M S, Amil M D. Interactive effect of rhizotrophic microorganisms on yield and nutrient uptake of chickpea(Cicer arietinum L.)[J]. European Journal of Agronomy, 2003, 19(1): 15-21.Doi:10.1016/S1161-0301(02)0015-1.
[11] 刘志明, 余玉冰, 秦碧霞, 等. 根结线虫幼虫致病细菌的筛选[J]. 中国生物防治, 2000, 16(3): 142.Doi:10.3321/j.issn.005-9261.2000.03.011 Liu Z M, Yu Y B, Qin B X, et al. Screening of the pathogenic bacteria of root knot nematode larva[J]. Chinese Journal of Biological Control, 2000, 16(3): 142.
[12] 郜春花, 王岗, 董云中, 等. 解磷菌剂盆栽及大田施用效果[J]. 山西农业科学, 2003, 31(3): 40-43.Doi:10.3969/j.issn.1002-2481.2003.03.010. Hao C H, Wang G, Dong Y Z, et al. Effect of phosphorus bacteria in the pot and the field[J]. Journal of Shanxi Agricultural Sciences, 2003, 31(3): 40-43.
[13] 吴鹏飞, 张冬明, 郝丽虹, 等. 解磷微生物研究现状及展望[J]. 中国农业科技导报, 2008, 10(3): 40-46.Doi:10.3969/j.issn.1008-0864.2008.03.007. Wu P F, Zhang D M, Hao L H, et al. Status quo and prospects of phosphate-soluble microorganisms[J]. Journal of Agricultural Science and Technology, 2008, 10(3): 40-46.
[14] 魏伟. 马尾松根际高效解磷真菌的筛选鉴定及其促生效应[D]. 南京: 南京林业大学, 2012. Wei W.Screening and identification of masson pine rhizosphere phosphate-solubilizing fungi and its application[D]. Nanjing: Nanjing Forestry University, 2012.
[15] 魏伟, 吴小芹, 乔欢. 马尾松根际高效解磷真菌的筛选鉴定及其促生效应[J]. 林业科学, 2014, 50(9): 82-88.Doi:10.11707/j.1001-7488.20140911. Wei W, Wu X Q, Qiao H. Screening and identification of phosphate-solubilizing fungi of Pinus massoniana rhizosphere and its application[J]. Scientia Silvae Sinicae, 2014, 50(9): 82-88.
[16] 乔欢, 吴小芹, 王早. 一株嗜松青霉JP-NJ4的解磷特性[J]. 微生物学通报, 2014, 41(9): 1741-1748.Doi:10.13344/j.microbiol.china.130828. Qiao H, Wu X Q, Wang Z. Phosphate-solubilizing characteristic of a Penicillium pinophilum strain JP-NJ4[J]. Microbiology China, 2014, 41(9): 1741-1748.
[17] 林先贵. 土壤微生物研究原理与方法[M]. 北京: 高等教育出版社, 2010. Lin X G. Principles and methods of soil microbiology research[M]. Beijing: Higher Education Press, 2010.
[18] 周娟. 山东省典型农产品基地土壤微生物多样性与地球化学元素关系研究[D]. 济南: 山东大学, 2008. Zhou J.Relationships between soil microbial diversity and geochemical elements in typical agricultural products bases in Shandong Province, China[D]. Ji'nan: Shandong University, 2008.
[19] 刘子雄, 朱天辉, 张健. 林木根系分泌物与根际微生物研究进展[J]. 世界林业研究, 2005, 18(6): 25-31.Doi:10.3969/j.issn.1001-424/2005.06.004. Liu Z X, Zhu T H, Zhang J. Research advances in root exudates and rhizosphere microorganisms of forest trees[J].World Forestry Research, 2005, 18(6): 25-31.
[20] Garland J L. Analysis and interpretation of community-level physiological profiles in microbial ecology[J]. FEMS Microbiology Ecology, 1997, 24(4): 289-300.Doi:10.1016/S0168-6496(97)00061-5.
[21] 高悦, 吴小芹, 孙民琴. 马尾松不同菌根苗对氮磷钾的吸收利用[J]. 南京林业大学学报(自然科学版), 2009, 33(4): 77-80.Doi:10.3969/j.issn.1000-2006.2009.04.016. Gao Y, Wu X Q, Sun M Q. Effects of ectomycorrhizal seedlings of masson pine on absorption and utilization of N, P and K[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2009, 33(4): 77-80.
[22] 刘俊杰. 磷浓度对大豆根际微生物群落结构及功能的影响[D]. 哈尔滨: 东北农业大学, 2009. Liu J J. Effects of different phosphorous concentration on microbial community structure and function in soybean rhizosphere[D]. Harbing: Northeast Agricultural University, 2009.
[23] Oberson A, Fardeau J C, Besson J M, et al. Soil phosphorus dynamics in cropping systems managed according to conventional and biological agriculturl methods[J]. Biology and Fertility of Solis, 1993, 16(2): 111-117.Doi:10.1007/bf00369411.
[24] 钟传青, 黄为一. 磷细菌 P17 对不同来源磷矿粉的溶磷作用及机制[J]. 土壤学报, 2004, 41(6): 931-937.Doi:10.3321/j.issn:0564-3929.2004.06.014. Zhong C Q, Huang W Y. Effects and mechanism of P-solubilizing Bacillus sp. 17 strain on phosphorus solubilization of different phosphate rocks[J]. Acta Pedologica Sinica, 2004, 41(6): 931-937.
[25] Cakmakci R, Donmez F, Aydin A, et al. Growth promotion of plants by plant growth-promoting rhizobacteria under greenhouse and two different field soil conditions[J]. Soil Biology and Biochemistry, 2006, 38(6): 1482-1487.Doi:10.1016/j.soilbia.2005.09.019.

两株解磷青霉对马尾松根际土壤的微生态效应及其促生作用

Effects of two phosphate-solubilizing Penicillium strains on microbial community structure and their growth-promoting in masson pine rhizosphere

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