南京林业大学学报(自然科学版) ›› 2016, Vol. 40 ›› Issue (06): 162-166.doi: 10.3969/j.issn.1000-2006.2016.06.025

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无机纳米颗粒在植物转化中的应用

霍爱玲1,2,陈金慧1,4,甄 艳1,夏 兵1,3,陈桢雨1,施季森1,4*   

  1. 1.林木遗传和生物技术省部共建教育部重点实验室,南京林业大学林学院,江苏 南京 210037;
    2.江苏淮阴师范学院, 江苏 淮安 223300;
    3.南京林业大学理学院,江苏 南京 210037;
    4.南方现代林业协同创新中心,江苏 南京 210037
  • 出版日期:2016-12-18 发布日期:2016-12-18
  • 基金资助:
    基金项目:国家高技术研究发展计划(2013AA102705); 高等教育博士课程专项基金项目(SRFDP 20113204130002); 江苏省高校自然科学基金项目(13KJA220001); 江苏高校优势学科建设工程资助项目(PAPD)
    第一作者:霍爱玲(huoailing_hn@163.com),博士生。
    *通信作者:施季森(jshi@njfu.edu.cn),教授。
    引文格式:霍爱玲,陈金慧,甄艳,等. 无机纳米颗粒在植物转化中的应用[J]. 南京林业大学学报(自然科学版),2016,40(6):162-166.

Inorganic nanoparticles as delivery vectors for plant transformation

HUO Ailing1,2,CHEN Jinhui1,4,ZHEN Yan1,XIA Bing1,3,CHEN Zhenyu1,SHI Jisen1,4*   

  1. 1.Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, College of Forestry, Nanjing Forestry University, Nanjing 210037,China;
    2. Huaiyin Normal University,Huaian,223300,China;
    3.College of Science, Nanjing Forestry University, Nanjing 210037,China;
    4. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing 210037, China
  • Online:2016-12-18 Published:2016-12-18

摘要: 随着纳米生物技术的发展,基于纳米材料构建基因载体的植物转基因技术,逐渐成为一类具有划时代意义的创新性高效植物转基因技术。笔者综述了羟基磷灰石、硅、碳纳米管、量子点、磁性纳米颗粒等无机纳米颗粒在植物转化中的应用,并比较了这些纳米载体的优势及存在的问题。分析认为,不同纳米材料对受体植物细胞的影响、纳米材料及其构建的载体入胞机制等基础理论问题迫切需要进一步阐明,入胞途径的细胞生物学和生理生化过程需要进一步实证,开发可定向输送目的基因到特定细胞或细胞器的安全高效新载体、目的基因的高效释放和功能激发等,将是未来一段时间内纳米植物生物技术研究的主要方向。

Abstract: With the development of nanotechnology, nano materials have been increasingly used for delivering genetic materials into plant cells for engineering purposes, which represent a major breakthrough in the 21st century. In this article, we reviewed the advancement of genetic engineering in plant using nanomaterials, including hydroxyapatite, silica, carbon nanotube, magnetite and quantum dots. The advantages and disadvantages of those materials were summarized. The effects of nanomaterials on the physiology of plant cells, as well as the mechanisms by which those nanomaterials are transported across cells are still largely unclear. The cellular biological and biochemistry mechanisms related to this process also need to be clarified. As a result, developing much safer nanomaterials that can specifically deliver genetic materials into target cells or even sub cellular compartments, with highly efficient payload release and functioning will be a main focus in future nanomaterial research.

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