【目的】鉴定龙爪槐枝干溃疡病的病原菌。【方法】采用组织分离法从龙爪槐枝干溃疡病病枝获得疑似病原菌,并通过野外致病性测定该病原菌,结合该病原菌的形态学特征、rDNA-ITS和EF-α基因序列分析,进行病原菌的种类鉴定。【结果】完成了F6菌株致病性分析的科赫法则实验,证实了其致病性; 鉴定该菌为腐皮镰孢菌复合种[Fusarium solani species complex(FSSC)]。【结论】腐皮镰孢菌复合种(FSSC)为引起龙爪槐枝干溃疡病的病原菌。
Abstract
【Objective】 Identify the pathogen causing stem canker on Sophora japonica Linn. var. japonica f. pendula Hort. 【Methods】 The method of tissue isolation was used to screen for suspected pathogens of stem canker on S. japonica Linn. var. japonica f. pendula Hort. A field experiment was performed to confirm the stem canker pathogen. Morphological characters, rDNA-ITS and EF-α gene sequence analyses were used to identify the pathogen causing this disease. 【Result】 A Koch's postulates assay was performed on the F6 isolate, and this fungal strain was identified as Fusarium solani species complex(FSSC).【Conclusion】F. solani species complex(FSSC)was confirmed as the pathogen of stem canker on S. japonica Linn. var. japonica f. pendula Hort. This is the first study describing the pathogen causing stem canker on S. japonica Linn. var. japonica f. pendula Hort.
关键词
龙爪槐枝干溃疡病 /
腐皮镰孢菌复合种(FSSC) /
症状 /
形态学特征 /
rDNA-ITS /
EF-α
Key words
Keywords:stem canker on Sophora japonica Linn. var. japonica f. pendula Hort. /
Fusarium solani species complex(FSSC) /
symptom /
morphological character /
rDNA-ITS /
EF-α
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基金
基金项目:江苏省农业科技自主创新资金项目(CX(16)1005); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:黄麟(lhuang@njfu.edu.cn),副教授,博士。*通信作者:叶建仁(jrye@njfu.edu.cn),教授,博士。
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