龙爪槐枝干溃疡病的病原研究

doi:10.3969/j.issn.1000-2006.201601004

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南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (05): 175-179.doi: 10.3969/j.issn.1000-2006.201601004

龙爪槐枝干溃疡病的病原研究

黄麟，杨济云，李秋成，张雅，叶建仁

南方现代林业协同创新中心,南京林业大学林学院,江苏南京 210037

出版日期:2017-10-18 发布日期:2017-10-18
基金资助:
基金项目:江苏省农业科技自主创新资金项目(CX(16)1005); 江苏高校优势学科建设工程资助项目(PAPD) 第一作者:黄麟(lhuang@njfu.edu.cn),副教授,博士。*通信作者:叶建仁(jrye@njfu.edu.cn),教授,博士。

Identification of the pathogen causing stem canker on Sophora japonica Linn. var. japonica f. pendula Hort.

HUANG Lin, YANG Jiyun, LI Qiucheng, ZHANG Ya, YE Jianren^*

Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Online:2017-10-18 Published:2017-10-18

摘要/Abstract

摘要： 【目的】鉴定龙爪槐枝干溃疡病的病原菌。【方法】采用组织分离法从龙爪槐枝干溃疡病病枝获得疑似病原菌,并通过野外致病性测定该病原菌,结合该病原菌的形态学特征、rDNA-ITS和EF-α基因序列分析,进行病原菌的种类鉴定。【结果】完成了F6菌株致病性分析的科赫法则实验,证实了其致病性; 鉴定该菌为腐皮镰孢菌复合种[Fusarium solani species complex(FSSC)]。【结论】腐皮镰孢菌复合种(FSSC)为引起龙爪槐枝干溃疡病的病原菌。

关键词: 龙爪槐枝干溃疡病, 腐皮镰孢菌复合种(FSSC), 症状, 形态学特征, rDNA-ITS, EF-α

Abstract: 【Objective】 Identify the pathogen causing stem canker on Sophora japonica Linn. var. japonica f. pendula Hort. 【Methods】 The method of tissue isolation was used to screen for suspected pathogens of stem canker on S. japonica Linn. var. japonica f. pendula Hort. A field experiment was performed to confirm the stem canker pathogen. Morphological characters, rDNA-ITS and EF-α gene sequence analyses were used to identify the pathogen causing this disease. 【Result】 A Koch's postulates assay was performed on the F6 isolate, and this fungal strain was identified as Fusarium solani species complex(FSSC).【Conclusion】F. solani species complex(FSSC)was confirmed as the pathogen of stem canker on S. japonica Linn. var. japonica f. pendula Hort. This is the first study describing the pathogen causing stem canker on S. japonica Linn. var. japonica f. pendula Hort.

Key words: Keywords:stem canker on Sophora japonica Linn. var. japonica f. pendula Hort., Fusarium solani species complex(FSSC), symptom, morphological character, rDNA-ITS, EF-α

中图分类号:

S763

黄麟,杨济云,李秋成,等. 龙爪槐枝干溃疡病的病原研究[J]. 南京林业大学学报（自然科学版）, 2017, 41(05): 175-179.

HUANG Lin, YANG Jiyun, LI Qiucheng, ZHANG Ya, YE Jianren. Identification of the pathogen causing stem canker on Sophora japonica Linn. var. japonica f. pendula Hort.[J].Journal of Nanjing Forestry University (Natural Science Edition), 2017, 41(05): 175-179.DOI: 10.3969/j.issn.1000-2006.201601004.

参考文献

[1] SHIDA H, UMINO T, TSUJI K, et al. Studies on the anti-hemorrhagic substances in herbs classified as hemostatics in Chinese medicine. X: on hemostatic activities of the parched herbs for hemostatics[J]. Yakugaku Zasshi, 1989, 109: 79.
[2] TANG Y, LOU F, WANG J, et al. Four new isoflavone triglycosides from Sophora japonica[J]. Journal of Natural Products, 2001, 64: 1107-1110.
[3] KIM J M, YUN-CHOI H S. Anti-platelet effects of flavonoids and flavonoid-glycosides from Sophora japonica[J]. Archives of Pharmacal Research, 2008, 31(7):886-890.DOI:10.1007/s12272-001-1242-1.
[4] 杨旺. 森林病理学[M]. 北京:中国林业出版社, 1996:147-148.
[5] 陈守常, 朱天辉, 杨佐忠. 四川森林病害[M]. 成都:四川科学技术出版社,2006: 473.
[6] 方中达.植病研究方法[M]. 3版. 北京:中国农业出版社,1998:122-124.
[7] HOU Z, XUE C, PENG Y, et al. A mitogen-activated protein kinase gene(MGV1)in Fusarium graminearum is required for female fertility, heterokaryon formation, and plant infection[J]. Molecular Plant-Microbe Interactions, 2002, 15: 1119-1127.
[8] 许志刚. 普通植物病理学[M]. 4版. 北京:中国农业出版社,2003:23-25.
[9] 陈剑山. 来自海南岛的镰刀菌的种类鉴定[D]. 儋州: 华南热带农业大学, 2007. CHEN J S. The identification of the Fusarium spp. from Hainan Island [D]. Danzhou:South China University of Tropical Agriculture, 2007.
[10] LESLIE J F, SUMMERELL B A, BULLOCK S. The Fusarium laboratory manual[M]. Iowa: Wiley-Blackwell, 2006: 250-255.
[11] DAMM U, MOSTERT L, CROUSS P W, et al. Novel Phaeoacremonium species associated with necrotic wood of Prunus trees[J]. Persoonia, 2008, 20:87-102.DOI:10.3767/003158508X324227.
[12] GARDES M, BRUNS T D. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts[J]. Molecular Ecology, 1993, 2:113-118.
[13] WHITE T J, BRUNS T, LEE J, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[C]//PCR protocols: a guide to methods and applications. San Diego, California:Academic Press, 1990:315-322.
[14] O'DONNELL K, KISTLER HC, CIGELNIK E, et al. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogis[J]. Proceedings of the National Academy of Sciences of the United States of America, 1998, 95:2044-2049.
[15] HUANG L, LI Q C, ZHANG Y, et al. Colletotrichum gloeosporioides sensu strict is a pathogen of leaf anthracnose on evergreen spindle tree(Euonymus japonicus)[J]. Plant Disease, 2016, 100:672-678.
[16] ZHANG N, O'DONNELL K, SUTTON D A, et al. Members of the Fusarium solani species complex that cause infections in both humans and plants are common in the environment[J]. Clinical Microbiology, 2006, 44(6): 2186-2190.
[17] O'DONNELL K. Molecular phylogeny of the Nectria haematococca-Fusarium solani species complex[J]. Mycologia, 2000, 92:919-938.

龙爪槐枝干溃疡病的病原研究

Identification of the pathogen causing stem canker on Sophora japonica Linn. var. japonica f. pendula Hort.

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