水拉恩氏菌JZ-GX1对杨树溃疡病菌的拮抗作用

doi:10.3969/j.issn.1000-2006.201603054

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南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (04): 42-48.doi: 10.3969/j.issn.1000-2006.201603054

水拉恩氏菌JZ-GX1对杨树溃疡病菌的拮抗作用

宋芳旭,吴小芹,赵群

南方现代林业协同创新中心,南京林业大学林学院,江苏省有害生物入侵预防与控制重点实验室,江苏南京 210037

出版日期:2017-08-18 发布日期:2017-08-18
基金资助:
收稿日期:2016-03-28 修回日期:2017-05-04
基金项目:国家林业公益性行业科研专项项目(201304404,201004061); 江苏省高校优势学科建设工程资助项目(PAPD)
第一作者:宋芳旭(sfxd11@126.com)。*通信作者:吴小芹(xqwu@njfu.edu.cn),教授。
引文格式:宋芳旭,吴小芹,赵群,等. 水拉恩氏菌JZ-GX1对杨树溃疡病菌的拮抗作用[J]. 南京林业大学学报(自然科学版),2017,41(4):42-48

Antagonism of plant growth-promoting bacteria Rahnella aquatilis JZ-GX1 to canker in poplar

SONG Fangxu, WU Xiaoqin^*, ZHAO Qun

Co-Innovation Center for the Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Online:2017-08-18 Published:2017-08-18

摘要/Abstract

摘要： 【目的】探讨根际促生细菌水拉恩氏菌(Rahnella aquatilis)JZ-GX1菌株对杨树溃疡病菌的拮抗作用。【方法】采用平板对抗、胞外代谢产物抑菌率测定、SEM电镜观察以及以‘NL895'杨(Populus×euram ericana CL‘NL895')1年生扦插苗为对象,采用茎干涂布和菌剂灌根处理等检测JZ-GX1菌株对杨树溃疡病的防治效果。【结果】水拉恩氏菌JZ-GX1菌体对杨树溃疡病菌金黄壳囊孢(Cytospora chrysosperma)和拟茎点霉(Phomopsis sp.)的抑菌效果明显,对七叶树壳梭孢(Fusicoccum aesculi)无抑制作用; 菌株胞外代谢产物对金黄壳囊孢也具有较好的抑菌效果,抑菌率达77.87%。在茎干涂布和菌剂灌根处理下,杨树盆栽苗的发病率较对照低,其中灌根法对金黄壳囊孢引起的溃疡病防效为84%。电镜观察发现,水拉恩氏菌JZ-GX1菌株发酵液能使金黄壳囊孢菌丝变稀且扭曲变形、分支增多; 抗菌物质检测可知,JZ-GX1菌株能够产生嗜铁素、纤维素酶和磷酸酯酶; 对病原菌细胞膜透性、可溶性糖和MDA有较大影响,处理后的金黄壳囊孢菌丝细胞的可溶性糖浓度(1 584.75 μmol/L)较对照降低,细胞膜透性(相对电导率达77.08%)和MDA含量(浓度达0.408 μmol/L)均高于对照,且JZ-GX1菌株能够在杨树体内定殖。【结论】水拉恩氏菌JZ-GX1菌株具有良好的对杨树溃疡病的生防潜力。

Abstract: 【Objective】 This study is to investigate the antagonistic ability and mechanism of Rahnella aquatilis JZ-GX1 strain against pathogens causing poplar canker to improve the biocontrol of polar canker. 【Method】 The control efficiency of JZ-GX1 strain was studied by antagonistic testing in dural culture, determining of the extracellular metabolites inhibitory rate, observation under SEM(scanning electron microscopy)and inoculation via root pouring and stem coating on one-year-old cuttings of ‘NL 895' polar(Populus×euramericana CL‘NL895'). 【Results】The results showed that R. aquatilis JZ-GX1 strain had significant antagonistic ability to C. chrysosperma and Phomopsis sp., but not to Fusicoccum aesculi. The extracellular metabolites of JZ-GX1 strain showed better antagonistic ability to Cytospora chrysosperma with 77.87% inhibitory rate. The occurrence rate of the disease was lower than the control under root pouring and stem coating treatment, and the control efficiency of JZ-GX1 strain against C. chrysosperma was 84%. The fermented suspension of JZ-GX1 strain can change the morphology of the hyphae of C. chrysosperma to be more thinned, twisted and more branches under SEM. JZ-GX1 strain also can produce siderophore, cellulase and phosphatase, which significantly affect the cell membrane permeability, the concentration of soluble sugar and MDA. The concentration of soluble sugar(1 584.75 μmol/L )decreased comparing to the control after treatment and the cell membrane permeability(with relative conductance of 77.08%)and MDA content increased MDA concentration of 0.408 μmol/L). JZ-GX1 strain can colonize in poplar. 【Conclusion】 Rahnella aquatilis JZ-GX1 strain had a potential to be used as bio-control agent against poplar canker.

中图分类号:

S763

宋芳旭,吴小芹,赵群. 水拉恩氏菌JZ-GX1对杨树溃疡病菌的拮抗作用[J]. 南京林业大学学报（自然科学版）, 2017, 41(04): 42-48.

SONG Fangxu, WU Xiaoqin, ZHAO Qun . Antagonism of plant growth-promoting bacteria Rahnella aquatilis JZ-GX1 to canker in poplar[J].Journal of Nanjing Forestry University (Natural Science Edition), 2017, 41(04): 42-48.DOI: 10.3969/j.issn.1000-2006.201603054.

参考文献

[1] 黄烈健, 苏晓华.我国杨树溃疡病研究进展[J].世界林业研究, 2003, 16(4): 49.DOI: 10.13348/j.cnki.sjlyyj.2003.04.010. HUANG L J, SU X H.The advance of canker disease-resistance breeding in poplar[J].World Forestry Research, 2003, 16(4): 49.
[2] 孙妍, 赵红盈, 宋小双, 等.豚草提取物对杨树溃疡病菌的抑制作用[J].安徽农业科学, 2014, 42(34): 12111-12112.DOI: 10.13989/ j.cnki.0517-6611.2014.34.038. SUN Y, ZHAO H Y, SONG X S, et al.Inhibition effect of crude extracts of Ambrosia artemisiifolia L.against Cytospora chrysosperma[J].Journal of Anhui Agri Sci, 2014, 42(34): 12111-12112.
[3] 陈越渠, 李立梅, 毛赫, 等.杨树溃疡病内生拮抗菌的筛选及鉴定[J].植物保护, 2015, 41(6): 126-131.DOI: 10.396/j.issn.0529-1542.2015.06.023. CHEN Y Q, LI L M, MAO H, et al.Screening of antagonistic bacteria of Valsa sordida and identification of the strain NS3[J].Plant Protection, 2015, 41(6): 126-131.
[4] LI G E, WU X Q, YE J R, et al.Isolation and identification of phytate-degrading rhizobacteria with activity of improving growth of poplar and Masson pine[J].World J Microbiol Biotechnol, 2013, 29: 2181-2193.DOI: 10.1007/s11274- 013-1384-3.
[5] 巢建国, 谈献和, 张瑜, 等.茅苍术快速繁殖[J].中药材, 2001, 24(7): 473.DOI: 10.13863/j.issn1001-4454.2001.07.003. CHAO J G, TAN X H, ZHANG Y, et al.The rapid propagation of Atractylodes lancea[J]. Journal of Chinese Materia Medica, 2001, 24(7): 473.
[6] 任嘉红.杨树溃疡病拮抗细菌的筛选及抗菌物质的研究[D].南京: 南京林业大学, 2009. REN J H.Screening of antagonistic bacteria and study on antibiotic substances of poplar canker disease[D].Nanjing:Nanjing Forestry University, 2009.
[7] 胡长松.水稻内生拮抗细菌的抗生作用及定殖研究[D].扬州: 扬州大学, 2006. HU C S. Antibiosis and colonization of endophytic antagonistic bacteria from rice[D].Yangzhou: Yangzhou University, 2006.
[8] 邢素芝, 汪建飞, 蔡丹.柠檬酸和苹果酸对小白菜种子萌发和细胞膜透性的影响[J].中国农学通报, 2007, 23(9): 312-316.DOI: 10.3969/j.issn.1000-6850.2007.09.067. XING S Z, WANG J F, CAI D.Effects of citrate and malate on the seed germination and membrane permeability of pakchoi[J].Chinese Agricultural Science Bulletin, 2007, 23(9): 312-316.
[9] 梁军, 王媛, 贾秀贞, 等.溃疡病菌对杨树愈伤组织细胞膜透性、可溶性糖MDA含量的影响[J].林业科学, 2008, 44(8): 72-77.DOI: 10.11707/j.1001-7488.20080813. LIANG J, WANG Y, JIA X Z, et al.Effects of infection with Botryosphaeria dothidea on cell membrane permeability, soluble sugar and MDA content in poplar calli[J].Scientia Silvae Sinicae, 2008, 44(8): 72-77.
[10] SCHWYN B, NEILANDS J B. Universal chemical assay for the detection and determination of siderophores[J].Analytical Biochemistry, 1987, 160(1): 47-56.DOI: 10.1016/0003-2697(87)90612-9.
[11] PIKOVSKAYA R I. Mobilization of phosphorus in soil in connection with vital activity of some microbial species[J].Mikrobiologiya, 1948, 17: 363-370.
[12] TEATHER R N, WOOD R J.Use of congored-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen[J].Applied and Environmental Microbiology, 1982, 43(4): 777-780.
[13] 陈天寿.微生物培养基的制造与应用[M].北京: 农业出版社, 1995: 44. CHEN T S.The manufacture and application of microbiological culture media[M].Beijing: China Agriculture Press, 1995:44.
[14] REIMMANN C, BEYELER M, LATIFI A.The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide and lipase[J].Molecular Microbiology, 1997, 24(2): 309-319. DOI:10.1046/j1365-2958.1997.3291701.x.
[15] 李桂娥.林木根际植酸盐降解细菌筛选及促生机理研究[D].南京: 南京林业大学, 2013. LI G E.Isolation and identification of phytate-degrading rhizobacteria and its plant growth promoting mechanisms[D].Nanjing: Nanjing Forestry University, 2013.
[16] 吉林省质量技术监督局. GB 22/T 1768-2013杨树溃疡病综合治理技术规程[S].北京: 中国标准出版社, 2013.
[17] 左胜涵.根际促生细菌水生拉恩氏JZ-GX1的解磷能力及其应用[D].南京: 南京林业大学, 2015. ZUO S H.Phosphate-solubilizing characteristic and application of PGPR Rahnella aquatilis JZ-GX1 strains[D].Nanjing: Nanjing Forestry University, 2015.
[18] 余贤美, 郑服丛, 林超, 等.土壤产嗜铁素拮抗细菌 CAS15的分离鉴定[J].植物保护学报, 2009, 36(2): 129-135.DOI: 10.13802/j.cnki.zwbhxb.2009.02.006. YU X M, ZHENG F C, LIN C, et al.Isolation and identification of siderphore producing bacteria CAS15 from the soil[J].Journal of Plant Protection, 2009, 36(2): 129-135.
[19] 费美娟, 陈建省, 王晓云.小麦叶片磷酸酯酶生化特性的研究[J].西北植物学报, 2006, 26(1): 110.DOI: 10.3321/j.issn: 1000-4025.2006.01.020. FEI M J, CHEN J S, WANG X Y.Biochemical properties of phosphatase in the leaves of wheat[J].Acta Botanica Boreali-Occidentalia Sinica, 2006, 26(1): 110.
[20] 陈凡.水拉恩氏菌HX2菌株防治葡萄根癌病的初步研究[D].北京: 中国农业大学, 2007. CHEN F.Study on antagonistic activity on grapevine crown gall by stain Rahnella aquatilis HX2[D].Beijing: China Agricultural University, 2007.
[21] ENTRY J A, CROMACK K J, KELSEY R G, et al.Response of Douglas-fir to infection by Armillaria ostoyae after thinning or thinning plus fertilization[J].Phytopathology, 1991, 81(6): 682-689. DOI: 10.1094/phyto-81-682.
[22] ENTRY J A, DONNELLY P K, CROMACK K J.Effect of nitrogen and carbon sources on lignin and cellulose degradation by Armillaria ostoyae after thinning or thinning plus fertilization[J].Phytopathology, 1991, 81(6): 682-689. DOI: 10.1094/phyto-81-682.
[22] ENTRY J A, DONNELLY P K, CROMACK K J.Effect of nitrogen and carbon sources on lignin and cellulose degradation by .Forest Pathology, 1993, 23(3): 129-137.DOI: 10.1111/j.1439-0329.1993.tb00952.x.
[23] LINDQUIST S, CRAIG E A.The heat-shock proteins[J].Annu Rev Genet, 1988, 22(1):631-677. DOI: 10.1146/annurev.ge.22.120188.003215.
[24] 潘华珍, 冯立明, 许彩民, 等.丙二醛对红细胞的作用[J].生物化学与生物物理进展, 1984, 2: 34-35. PAN H Z, FENG L M, XU C M, et al.Effects of malondialdehyde on red blood cells[J]. Progress in Biochemistry and Biophysics, 1984, 2: 34-35.

水拉恩氏菌JZ-GX1对杨树溃疡病菌的拮抗作用

Antagonism of plant growth-promoting bacteria Rahnella aquatilis JZ-GX1 to canker in poplar

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