青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究

doi:10.3969/j.issn.1000-2006.201610064

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (04): 6-12.doi: 10.3969/j.issn.1000-2006.201610064

青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究

徐静珠¹,吴彩娥^1,2*,应瑞峰¹,李婷婷¹,范龚健¹,方升佐^2,3

1.南京林业大学轻工科学与工程学院,江苏南京 210037;
2.南方现代林业协同创新中心, 江苏南京 210037;
3.南京林业大学林学院,江苏南京 210037

出版日期:2017-08-18 发布日期:2017-08-18
基金资助:
收稿日期:2016-10-31 修回日期:2017-02-18
基金项目:国家自然科学基金项目(31270673); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:徐静珠(1551289377@qq.com)。*通信作者:吴彩娥(sxwucaie@163.com),教授,博士。
引文格式:徐静珠,吴彩娥,应瑞峰,等. 青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究[J]. 南京林业大学学报(自然科学版),2017,41(4):6-12.

Hypoglycemic and antioxidant activity of various polysaccharides from Cyclocarya paliurus leaves in vitro

XU Jingzhu¹, WU Caie^1,2*, YING Ruifeng¹, LI Tingting¹, FAN Gongjian¹,FANG Shengzuo^2,3

1.College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, China;
2.Co-Innovation Centre for Sustainable Forestry in Southern China, Nanjing 210037, China;
3.College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Online:2017-08-18 Published:2017-08-18

摘要/Abstract

摘要： 【目的】研究青钱柳叶多糖的体外降血糖及抗氧化活性。【方法】以对α-葡萄糖苷酶的抑制作用表示青钱柳叶多糖体外降血糖活性,以总抗氧化能力、清除DPPH自由基、羟基自由基能力和Fe²⁺螯合能力评价其体外抗氧化活性。【结果】青钱柳叶多糖各组分对α-葡萄糖苷酶均有一定的抑制作用,且呈现一定的剂量-效应关系。不同醇沉多糖组分中,50%醇沉组分对α-葡萄糖苷酶的抑制作用最强; 青钱柳叶多糖各组分均具有一定的抗氧化活性,不同醇沉组分清除DPPH自由基能力以及总抗氧化能力由强到弱依次为50% 组分、60% 组分、70%组分、80%组分,而清除羟基自由基能力以及对Fe²⁺螯合能力的强弱正好相反。【结论】青钱柳叶中含有的多糖组分具有良好的体外降血糖和抗氧化活性,可进一步加工利用。

Abstract: 【Objective】In order to evaluate the hypoglycemic and antioxidant activity of polysaccharides from Cyclocarya paliurus leaves in vitro. The activity of refined polysaccharides and precipitation fractionations of purified C. paliurus polysaccharide obtained from different concentrations of ethanol were compared in this study.【Method】The activity of inhibiting α-glucosidase was used to evaluate the hypoglycemic effect in vitro. The ability of total antioxidant, scavenging DPPH and(·OH)radicals and chelating Fe²⁺were used to evaluate the antioxidant capacity.【Result】The results showed that refined polysaccharides of C. paliurus and different precipitation fractionations all showed a high inhibition against α-glucosidase, and showed a dose-effect relationship. With the ethanol concentration increasing, the fractions obtained showed a higher inhibition activity. Refined polysaccharides of C. paliurus and different precipitation fractionations all showed a good antioxidant activity. Among different precipitation fractionations, the abilities of scavenging DPPH and total antioxidant were increased with the ethanol concentration increasing. In contrast, the abilities of scavenging(·OH)radicals and chelating Fe²⁺ were decreased.【Conclusion】Different C. paliurus polysaccharides have good in vitro hypoglycemic and antioxidant activity, which can be used for further manufacture and expoliting.

中图分类号:

徐静珠,吴彩娥,应瑞峰,等. 青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究[J]. 南京林业大学学报（自然科学版）, 2017, 41(04): 6-12.

XU Jingzhu, WU Caie, YING Ruifeng, LI Tingting, FAN Gongjian,FANG Shengzuo. Hypoglycemic and antioxidant activity of various polysaccharides from Cyclocarya paliurus leaves in vitro[J].Journal of Nanjing Forestry University (Natural Science Edition), 2017, 41(04): 6-12.DOI: 10.3969/j.issn.1000-2006.201610064.

参考文献

[1] 华思敏, 徐思绮, 管舒杰, 等. 不同工艺黑木耳多糖的抗氧化与糖苷酶抑制作用研究[J]. 中国食品添加剂, 2016,63(4):63-68. DOI: 10.3969/j.issn.1006-2513.2016.04.002. HUA S M, XU S Q, GUAN S J, et al. Studies on the effects of different polysaccharides extraction process from Auricularia auricula on antioxidant activities and α-glycosidase inhibitory[J]. China Food Additives, 2016,63(4):63-68.
[2] 刘杨, 刘仲华. 苦菜降血糖作用及机理研究进展[J]. 食品研究与开发, 2014, 35(22):132-134. DOI: 10.3969/j.issn.1005-6521.2014.22.037. LIU Y, LIU Z H. Sowthistle hyperglycemic effect and mechanism progress[J]. Food Research and Development, 2014, 35(22):132-134.
[3] 许春平, 杨琛琛, 郑坚强, 等. 植物叶多糖的提取和生物活性综述[J]. 食品研究与开发, 2014,35(14):111-114. DOI: 10.3969/j.issn.1005-6521.2014.14.33. XU C P, YANG C C, ZHEN J Q, et al. Review on extraction and biological activity of polysaccharides from plant leave[J]. Food Research and Development, 2014, 35(14):111-114.
[4] 于滨, 马晓燕, 李丹丹, 等. 苦瓜降血糖成分及机制研究进展[J]. 中国果菜, 2013(3):47-52. DOI: 10.3969/j.issn.1008-1038.2013.03.020. YU B, MA X Y, LI D D, et al. Advances in research on hypoglycemic composition and mechanism of Bitter[J]. China Fruit and Vegetable, 2013(3):47-52.
[5] 谢明勇, 谢建华. 青钱柳研究进展[J]. 食品与生物技术学报, 2008, 27(1):113-121. DOI: 10.3321/j.issn:1673-1689.2008.01.021. XIE M Y, XIE J H. Review about the research on Cyclocarya paliurus(Batal.)Iljinskaja[J]. Journal of Food Science and Biotechnology, 2008, 27(1):113-121.
[6] 沈兵. 青钱柳中α-葡萄糖苷酶抑制剂的研究[J]. 浙江大学学报(农业与生命科学版), 2014,40(3):275-280. DOI: 10.3785/j.issn.1008-9209.2014.03.005. SHEN B. Study on an α-glucosidase inhibitor separated from Cyclocarya paliurus leaves[J]. Journal of Zhejiang University(Agriculture and Life Sciences), 2014, 40(3):275-280.
[7] WANG Q Q, JIANG C H, FANG S Z, et al. Antihyperglycemic, antihyperlipidemic and antioxidant effects of ethanol and aqueous extracts of Cyclocarya paliurus leaves in type 2 diabetic rats[J]. Journal of Ethnopharmacology, 2013, 150(3): 1119-1127. DOI: 10.1016/j.jep.2013.10.040.
[8] 上官新晨, 陈木森, 蒋艳, 等. 青钱柳多糖降血糖活性的研究[J]. 食品科技, 2010, 35(3):82-84. SHANGGUAN X C, CHEN M S, JIANG Y, et al. Study on hypoglycemic activity of polysaccharide from Cyclocarya paliurus(Batal.)Iljinskaja leaves[J]. Food Science and Technology, 2010, 35(3):82-84.
[9] 胡文兵, 赵静, 陈婷婷, 等. 青钱柳多糖对高脂血症小鼠的降血脂作用及机制初探[J]. 现代食品科技, 2015,31(11):39-44. HU W B, ZHAO J, CHEN T T, et al. Study on hypolipidemic effect and mechanism of Cyclocarya paliurus(Batal.)Iljinskaja polysaccharide in hyperlipidemic mice[J]. Modern Food Science & Technol-ogy, 2015,31(11):39-44.
[10] 李楠, 赵静, 吴茹, 等. 青钱柳多糖对高脂血症小鼠脂代谢及PPAR γ、ATGL基因mRNA表达的影响[J]. 中国食品学报, 2015,15(9):9-14. DOI: 10.16429/j.1009-7848.2015.09.002. LI N, ZHAO J, WU R, et al. Research on the influence of Cyclocarya paliurus(Batal.)Iljinskaja polysaccharide on PPAR γ and ATGL gene expression in Hyperlipoidemia Mice[J]. Journal of Chinese I-nstitute of Food Science and Technology, 2015,15(9):9-14.
[11] 黄明圈, 上官新晨, 徐明生, 等. 青钱柳多糖降血脂作用的研究[J]. 江西农业大学学报, 2011,33(1):157-161. DOI: 10.3969/j.issn.1000-2286.2011.01.029. HUANG M Q, SHANGGUAN X C, XU M S, et al. Studies on hypolipemic effect of Cyclocarya paliurus(Batal)Iljinskaja Polysaccharide[J]. Acta Agriculturae Universitis Jiangxiensis, 2011,33(1):157-161.
[12] XIE J H, XIE M Y, NIE S R, et al. Isolation, chemical composition and antioxidant activities of a water-soluble polysaccharide from Cyclocarya paliurus(Batal.)Iljinskaja[J]. Food Chemistry, 2010, 119(4):1626-1632. DOI:10.1016/j.foodchem.2009.09.055.
[13] XIE J H, WANG Z J, SHEN M Y, et al. Sulfated modification, characterization and antioxidant activities of polysaccharide from Cyclocarya paliurus[J]. Food Hydrocolloids, 2016, 91:1073-1080. DOI: 10.1016/j. foodhyd.2015.02.018.
[14] XIE J H, LIU X, SHEN M Y, et al. Purification, physicochemical characterisation and anticancer activity of a polysaccharide from Cyclocarya paliurus leaves[J]. Food Chemistry, 2013, 136(3-4): 1453-1460. DOI: 10.1016/j.foodchem.2012.09.078.
[15] 李磊, 谢明勇, 易醒, 等. 青钱柳多糖组分及其降血糖活性研究[J]. 江西农业大学学报, 2001, 23(4): 484-486. DOI: 10.3969/j.issn.1000-2286.2001.04.008. LI L, XIE M Y, YI X, et al. Bioactivity of polysaccharide from Cyclocarya paliurus(Batal.)Iljinsk leaves in reducing blood sugar[J]. Acta Agriculturae Universitis Jiangxiensis, 2001, 23(4): 484-486.
[16] 谢建华, 谢明勇, 聂少平, 等. 苯酚-硫酸法测定青钱柳中多糖含量[J]. 食品工业, 2010(4): 93-95. XIE J H, XIE M Y, NIE S P, et al. Phenol-Sulfuric acid method for determination of polysaccharide content in Cyclocarya paliurus(Batal.)Iljinsk leaves[J]. Food Industry, 2010(4): 93-95.
[17] 郭金龙, 陈有君, 孙国琴, 等. 苯酚-硫酸法测定杏鲍菇多糖方法的研究[J]. 食品科学, 2008,29(12): 555-558. DOI: 10.3321/j.issn:1002-6630.2008.12.129. GUO J L, CHEN Y J, SUN G Q, et al. Study on Phenol-Sulfuric acid method for determination of polysaccharide content in Pleurotus eryngii[J]. Food Sciences, 2008,29(12): 555-558.
[18] 王永刚, 王世伟, 李云春, 等. 考马斯亮蓝G-250与牛血清白蛋白的相互作用研究[J]. 现代食品科技, 2016,32(1):89-94. WANG Y G, WANG S W, LI Y C, et al. Study on the interaction of coomassie brilliant blue G-250 with bovine serum albumin[J]. Modern Food Science & Technology, 2016,32(1):89-94.
[19] 王艾平, 周丽明. 考马斯亮蓝法测定茶籽多糖中蛋白质含量条件的优化[J]. 河南农业科学, 2014, 43(3):150-153. DOI: 10.3969/j.issn.1004-3268.2014.03.033. WANG A P, ZHOU L M. Determination of protein content in Camellia oleifera seed polysaccharides by Coomassie brilliant blue method[J]. Journal of Henan Agricultural Sciences, 2014, 43(3):150-153.
[20] 吕欢, 罗明琍, 方飞, 等. 桑叶提取物对体外α-葡萄糖苷酶活性的影响[J]. 时珍国医国药, 2012,23(1):41-42. DOI: 10.3969/j.issn.1008-0805.2012.01.019. LYU H, LUO M L, FANG F, et al. In vitro inhibitory effect of Mulberry leaf extract on α-glucosidase activity[J]. Lishizhen Medicine and Materia Medica Research, 2012,23(1):41-42.
[21] ZHANG S, LI X Z. Inhibition of α-glucosidase by polysaccharides from the fruit hull of Camellia oleifera Abel[J]. Carbohydrate Polymers, 2015, 115:38-43. DOI: 10.1016/j.carbpol.2014.08.059.
[22] ZHANG Z L, KONG F S, NI K H, et al. Structural characterization, α-glucosidase inhibitory and DPPH scavenging activities of polysaccharides from Guava[J]. Carbohydrate Polymers, 2016, 144:106-114. DOI: 10.1016/j.carbpol.2016.02.030.
[23] XIE J H, DONG C J, NIE S P, et al. Extraction, chemical composition and antioxidant activity of flavonoids from Cyclocarya paliurus(Batal.)Iljinskaja leaves[J]. Food Chemistry, 2015, 186:97-105. DOI: 10.1016/j.foodchem.2014.06.106.
[24] 李广富, 陈伟, 范路平, 等. 茶新菇多糖酸奶体外抗氧化与抗衰老效果[J]. 现代食品科技, 2015,31(10): 1-8. LI G F, CHEN W, FAN L P, et al. In vitro antioxidant and anti-aging effects of Agrocybe polysaccharide yogurt[J]. Modern Food Science & Technology, 2015,31(10):1-8.
[25] ZHONG K, LIN W J, WANG Q, et al. Extraction and radicals scavenging activity of polysaccharides with microwave extraction from Mung bean hulls[J]. International Journal of Biological Macromolecules, 2012, 51(4):612-617. DOI: 10.1016/j.ijbiomac.2012.06.032.
[26] 徐运飞, 刘琴, 宋珅, 等. 响应面法优化黄参多糖的提取工艺及其体外抗氧化活性[J]. 天然产物研究与开发, 2015,27(12):2116-2123. XU Y F, LIU Q, SONG S, et al. Optimization of extraction process by response surface methodology and antioxidant activity of polysaccharide from Sphallerocarpus racills[J]. Natural Product Research and Development, 2015,27,(12):2116-2123.
[27] ZHONG K, WANG Q, HE Y, et al. Evaluation of radicals scavenging, immunity-modulatory and antitumor activities of Longan polysaccharides with ultrasonic extraction on in S180 tumor mice models[J]. International Journal of Biological Macromolecules, 2010, 47(3):356-360. DOI:10.1016/j.ijbiomac.2010.05.022.
[28] LI X Y, WANG L, WANG Y, et al. Effect of drying method on physicochemical properties and antioxidant activities of Hohenbuehelia serotina polysaccharides[J]. Process Biochemistry, 2016, 51(8):1100-1108. DOI: 10.1016/j.procbio.2016.05.006.
[29] 张红霞, 杨丹丹, 王凤, 等. 杜仲叶乙醇提取物的降糖作用机理[J]. 食品科学, 2014, 35(17):197-203.DOI:10.7506/spkx1002-6630-201417038. ZHANG H X, YANG D D, WANG F, et al. Ethanol extract of Eucommia ulmoides leaves inhibits alpha-glucosidase in Caco-2 cells[J]. Food Sciences, 2014, 35(17):197-203.
[30] 秦健,刘洋,方升佐, 等.光质和光强对青钱柳生长和抗氧化酶活性的影响[J].南京林业大学学报(自然科学版),2017,41(4):13-18.DOI:10.3969/j.issn.1000-2006.201610001. QIN J, LIU Y, FANG S Z, et al. Effects of light quality and intensity on growth and antioxidative activities of Cyclocarya paliurus seedlings[J]. Journal of Nanjing Forestry University(Natural Sciences Edition),2017,41(4):13-18.
[31] XIE J H, SHEN M Y, XIE M Y, et al. Ultrasonic-assisted extraction, antimicrobial and antioxidant activities of Cyclocarya paliurus(Batal.)Iljinskaja polysaccharides[J]. Carbohydrate Polymers, 2012, 89(7):177-184. DOI: 10.1016/j.carbpol.2012.02.068.

青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究

Hypoglycemic and antioxidant activity of various polysaccharides from Cyclocarya paliurus leaves in vitro

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价

作者

读者

审稿