青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究

徐静珠; 吴彩娥; 应瑞峰; 李婷婷; 范龚健; 方升佐

doi:10.3969/j.issn.1000-2006.201610064

徐静珠,吴彩娥,应瑞峰,李婷婷,范龚健,方升佐

南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (04) : 6-12.

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南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (04) : 6-12. DOI: 10.3969/j.issn.1000-2006.201610064

专题报道

青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究

徐静珠¹,吴彩娥^1,2*,应瑞峰¹,李婷婷¹,范龚健¹,方升佐^2,3

作者信息 +

Hypoglycemic and antioxidant activity of various polysaccharides from Cyclocarya paliurus leaves in vitro

XU Jingzhu¹, WU Caie^1,2*, YING Ruifeng¹, LI Tingting¹, FAN Gongjian¹,FANG Shengzuo^2,3

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文章历史 +

摘要

【目的】研究青钱柳叶多糖的体外降血糖及抗氧化活性。【方法】以对α-葡萄糖苷酶的抑制作用表示青钱柳叶多糖体外降血糖活性,以总抗氧化能力、清除DPPH自由基、羟基自由基能力和Fe²⁺螯合能力评价其体外抗氧化活性。【结果】青钱柳叶多糖各组分对α-葡萄糖苷酶均有一定的抑制作用,且呈现一定的剂量-效应关系。不同醇沉多糖组分中,50%醇沉组分对α-葡萄糖苷酶的抑制作用最强; 青钱柳叶多糖各组分均具有一定的抗氧化活性,不同醇沉组分清除DPPH自由基能力以及总抗氧化能力由强到弱依次为50% 组分、60% 组分、70%组分、80%组分,而清除羟基自由基能力以及对Fe²⁺螯合能力的强弱正好相反。【结论】青钱柳叶中含有的多糖组分具有良好的体外降血糖和抗氧化活性,可进一步加工利用。

Abstract

【Objective】In order to evaluate the hypoglycemic and antioxidant activity of polysaccharides from Cyclocarya paliurus leaves in vitro. The activity of refined polysaccharides and precipitation fractionations of purified C. paliurus polysaccharide obtained from different concentrations of ethanol were compared in this study.【Method】The activity of inhibiting α-glucosidase was used to evaluate the hypoglycemic effect in vitro. The ability of total antioxidant, scavenging DPPH and(·OH)radicals and chelating Fe²⁺were used to evaluate the antioxidant capacity.【Result】The results showed that refined polysaccharides of C. paliurus and different precipitation fractionations all showed a high inhibition against α-glucosidase, and showed a dose-effect relationship. With the ethanol concentration increasing, the fractions obtained showed a higher inhibition activity. Refined polysaccharides of C. paliurus and different precipitation fractionations all showed a good antioxidant activity. Among different precipitation fractionations, the abilities of scavenging DPPH and total antioxidant were increased with the ethanol concentration increasing. In contrast, the abilities of scavenging(·OH)radicals and chelating Fe²⁺ were decreased.【Conclusion】Different C. paliurus polysaccharides have good in vitro hypoglycemic and antioxidant activity, which can be used for further manufacture and expoliting.

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徐静珠,吴彩娥,应瑞峰,李婷婷,范龚健,方升佐. 青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究[J]. 南京林业大学学报（自然科学版）. 2017, 41(04): 6-12 https://doi.org/10.3969/j.issn.1000-2006.201610064

XU Jingzhu, WU Caie, YING Ruifeng, LI Tingting, FAN Gongjian,FANG Shengzuo. Hypoglycemic and antioxidant activity of various polysaccharides from Cyclocarya paliurus leaves in vitro[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2017, 41(04): 6-12 https://doi.org/10.3969/j.issn.1000-2006.201610064

中图分类号： TS 201.4 S713

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基金

收稿日期:2016-10-31 修回日期:2017-02-18
基金项目:国家自然科学基金项目(31270673); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:徐静珠(1551289377@qq.com)。*通信作者:吴彩娥(sxwucaie@163.com),教授,博士。
引文格式:徐静珠,吴彩娥,应瑞峰,等. 青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究[J]. 南京林业大学学报(自然科学版),2017,41(4):6-12.