Abstract: 【Objective】Somatic embryogenesis and plant regeneration of nematode-resistant Pinus densiflora were studied for obtaining a large number of embryogenic callus. 【Method】We used embryogenic calluses 22#-1 and 13#-1 of nematode-resistant P. densiflora as material. First, cell structure of callus was observed under a stereomicroscope. Then we evaluated how combination of hormone, genotype, subculture modes and times affected callus proliferation and biomass. 【Result】The results showed that successful maintenance and proliferation of embryogenic callus depended heavily on plant growth regulating substances, genotype, subculture modes and times. Embryogenic callus of nematode-resistant P. densiflora was classified into two categories: embryogenic callus containing embryonic suspensor mass and non-embryogenic callus. The optimal combination of hormone was 2.0 mg/L 6-BA and 4.0 mg/L NAA. The proliferations of embryogenic calluses in different genotypes were different. The rate of proliferation of clonal embryo callus 22#-1 was slightly faster and 13#-1 was slower, but the difference was not significant. Liquid -solid and solid alternate culture mode was the most suitable proliferation mode. 【Conclusion】we should select embryogenic callus to subculture and the optimal combination of hormone was 2.0 mg/L 6-BA and 4.0 mg/L NAA. The calluses should be cultured in a liquid -solid and solid alternate mode.