山樱花群体遗传多样性的SSR分析

伊贤贵; 陈洁; 尤禄祥; 从睿; 王华辰; 段一凡; 王贤荣

doi:10.3969/j.issn.1000-2006.201702036

山樱花群体遗传多样性的SSR分析

伊贤贵,陈洁,尤禄祥,从睿,王华辰,段一凡,王贤荣

南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (05) : 25-31.

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南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (05) : 25-31. DOI: 10.3969/j.issn.1000-2006.201702036

研究论文

山樱花群体遗传多样性的SSR分析

伊贤贵,陈洁,尤禄祥,从睿,王华辰,段一凡,王贤荣^*

作者信息 +

Genetic divetsity of Cerasus serrulata populations assessed by SSR markers

YI Xiangui, CHEN Jie, YOU Luxiang, CONG Rui, WANG Huachen,DUAN Yifan, WANG Xianrong^*

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摘要

【目的】分析山樱花不同居群遗传多样性,为其种质资源保护与利用提供理论依据。【方法】利用SSR分子标记对8个山樱花自然居群共224份样本进行遗传多样性分析。【结果】17对SSR引物共检测到113个等位基因,平均每个位点6.647个等位基因,多态位点百分率为92.65%; 物种水平上Nei's遗传多样性指数为0.634,居群水平上Nei's遗传多样性指数、Shannon信息指数分别为0.498、0.939。Structure分析显示8个山樱花居群来自3个基因库,与主坐标分析结果较为一致。【结论】SSR标记可以用于山樱花群体遗传多样性分析,山樱花群体间与群体内遗传多样性均较高。

Abstract

【Objective】Cerasus serrulata(Lindley)Loudon, belonging to the Cerasus genus in the Rosaceae family,possesses renowned ornamental value along with rich and superior genetic characters for developing new flowering cherry cultivars. The present study aimed to reveal its genetic diversity and provide a theoretical basis for protecting and utilizing good C. serrulata resources.【Method】SSR markers were used to evaluate the genetic diversity of 224 C. serrulata indivi-duals from eight populations.【Result】A total of 113 alleles were detected from 17 pairs of SSR primes. The percentage of polymorphic loci was 92.65%. Nei's genetic diversity indexes at the species and population level were, respectively, 0.634 and 0.498. The Shannon diversity index was 0.939. Furthermore, the structure analysis showed that the eight po-pulations were from three different gene pools and the results predominantly agreed with the principal coordinate analysis.【Conclusion】SSR markers were used to effectively conduct a genetic diversity analysis of C. serrulata and revealed the high genetic diversity within and between C. serrulata populations.

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伊贤贵,陈洁,尤禄祥,从睿,王华辰,段一凡,王贤荣. 山樱花群体遗传多样性的SSR分析[J]. 南京林业大学学报（自然科学版）. 2018, 42(05): 25-31 https://doi.org/10.3969/j.issn.1000-2006.201702036

YI Xiangui, CHEN Jie, YOU Luxiang, CONG Rui, WANG Huachen,DUAN Yifan, WANG Xianrong. Genetic divetsity of Cerasus serrulata populations assessed by SSR markers[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2018, 42(05): 25-31 https://doi.org/10.3969/j.issn.1000-2006.201702036

中图分类号： S718.46

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基金

收稿日期:2017-02-22 修回日期:2018-04-18 基金项目:江苏省林业“三新”工程项目(LYSX[2015]17); 江苏省林业科技创新与推广项目(LYKJ[2017]14); 江苏省现代农业推广项目(BE2017037) 第一作者:伊贤贵(354067272@qq.com),讲师。*通信作者:王贤荣(wangxianrong66@njfu.edu.cn),教授。