紫雨桦耐盐性及花色苷合成相关基因的表达特性

doi:10.3969/j.issn.1000-2006.201704048

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南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (02): 25-32.doi: 10.3969/j.issn.1000-2006.201704048

紫雨桦耐盐性及花色苷合成相关基因的表达特性

吕东林,林琳,郭译文,韩锐,姜静^*

林木遗传育种国家重点实验室,东北林业大学,黑龙江哈尔滨 150040

出版日期:2018-04-12 发布日期:2018-04-12
基金资助:
基金项目:大学生创新训练项目(201610225013) 第一作者:吕东林(1247823654@qq.com)。*通信作者:姜静(jiangjing1960@126.com),教授。

Characterization of gene expression in anthocyanin synthesis and salt tolerance of Betula pendula ‘Purple Rain'

LYU Donglin,LIN Lin,GUO Yiwen, HAN Rui,JIANG Jing^*

State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040, China

Online:2018-04-12 Published:2018-04-12

摘要/Abstract

摘要： 【目的】探讨紫雨桦的耐盐性与花色素苷合成相关基因的相关性,为揭示紫雨桦的耐盐机理提供参考。【方法】以紫雨桦和垂枝桦(对照)为试材,测定盐胁迫下其叶绿素荧光参数及生理指标的变化,同时采用qRT-PCR方法分析紫雨桦和垂枝桦中查耳酮合酶基因(CHS)、二氢黄酮醇还原酶基因(DFR)、花色素苷合成基因及MYB转录因子家族基因的表达特性。【结果】经质量分数0.8%NaCl胁迫12 d后,紫雨桦叶片花青素含量指数显著高于垂枝桦,是垂枝桦的3.4倍,其盐害指数显著低于垂枝桦,胁迫12 d的联苯胺蓝(DAB)和氮蓝四唑(NBT)染色显示,紫雨桦叶片中O^-₂和H₂O₂的累积少于垂枝桦; 在叶绿素荧光参数方面,NaCl胁迫12 d时,紫雨桦的F_v/F_m值仍在正常值范围内,与胁迫0 d比较,其qP和Φ_PS_Ⅱ值的降幅也显著小于垂枝桦(P<0.05),紫雨桦的NPQ较0 d提高了282.29%,升幅显著高于垂枝桦(P<0.01)。 0.8%NaCl胁迫下CHS、DFR花色素苷合成基因及MYB转录因子家族基因的表达特性是:紫雨桦中的BpMYB10、BpCHS2和BpDFR5基因表达规律明显不同于垂枝桦,NaCl胁迫下上述3条基因持续高表达。【结论】富含花青素的紫雨桦能够耐受一定浓度范围的NaCl胁迫,紫雨桦中的BpMYB10、BpCHS2和BpDFR5基因表达规律明显不同于垂枝桦,这些基因可能与花青素的合成密切相关,花色苷可增强紫雨桦清除活性氧自由基的能力,减缓膜质氧化,提高其耐盐性。

Abstract: 【Objective】 To understand the mechanism of salt tolerance, we examined a possible relationship between salt tolerance and gene expression for anthocyanin biosynthesis in salt tolerant Betula pendula ‘Purple Rain'.【Methods】 We used Betula pendula ‘Purple Rain' and Betula pendula ‘Roth'(control)to measure chlorophyll fluorescence and other physiological indicators under salt stress. We analyzed gene expression of chalcone synthase(CHS), dihydlroflavonol-4-reductase(DRF), and MYB transcription factor related to anthocyanin biosynthesis using RT-PCR. 【Result】 The results showed that anthocyanin was 3.4 times higher in ‘Purple Rain,' whereas salt damage indexes were significantly lower than those of ‘Roth' after 12 d under 0.8% NaCl stress. The reswlts of DAB and NBT staining showed that accumulation of O^-₂ and H₂O₂ in leaves of ‘Purple Rain' was lower than that of ‘Roth'. The F_v/F_m value for ‘Purple Rain' was within the normal range after 12 d under NaCl stress, and the qP and Φ_PS_Ⅱ values were significantly lower than those of ‘Roth'(P < 0.05). The NPQ was 282.29% and was significantly higher than that of ‘Roth'(P < 0.01). Gene expression of BpCHS2,BpDFR5, and BpMYB10, related to anthocyanin biosynthesis, were continuously up-regulated under NaCl stress. 【Conclusion】 Anthocyanin-rich ‘Purple Rain' can tolerate a certain range of NaCl stress. The BpMYB10, BpCHS2, and BpDFR5 of ‘Purple Rain' exhibited a different expression pattern in comparison with ‘Roth', and this might be closely related to anthocyanin biosynthesis. Anthocyanin can enhance the ability of ‘Purple Rain' to remove reactive oxygen radicals, slow down oxidation of membranes, and improve salt tolerance.

中图分类号:

S722.5

吕东林,林琳,郭译文,等. 紫雨桦耐盐性及花色苷合成相关基因的表达特性[J]. 南京林业大学学报（自然科学版）, 2018, 42(02): 25-32.

LYU Donglin,LIN Lin,GUO Yiwen, HAN Rui,JIANG Jing. Characterization of gene expression in anthocyanin synthesis and salt tolerance of Betula pendula ‘Purple Rain'[J].Journal of Nanjing Forestry University (Natural Science Edition), 2018, 42(02): 25-32.DOI: 10.3969/j.issn.1000-2006.201704048.

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紫雨桦耐盐性及花色苷合成相关基因的表达特性

Characterization of gene expression in anthocyanin synthesis and salt tolerance of Betula pendula ‘Purple Rain'

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