高盆樱桃的组培快繁研究

张帆; 叶露; 董姬妃; 万雪琴; 钟宇

doi:10.3969/j.issn.1000-2006.201706047

高盆樱桃的组培快繁研究

张帆,叶露,董姬妃,万雪琴,钟宇

南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (03) : 111-116.

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南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (03) : 111-116. DOI: 10.3969/j.issn.1000-2006.201706047

研究论文

高盆樱桃的组培快繁研究

张帆¹,叶露¹,董姬妃¹,万雪琴^2*,钟宇²

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Study on tissue culture and rapid propagation of Cerasus cerasoides var. cerasoides

ZHANG Fan¹, YE Lu¹, DONG Jifei¹, WAN Xueqin^2*, ZHONG Yu²

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摘要

【目的】通过对高盆樱桃外植体灭菌方法的筛选和培养条件的优化,解决高盆樱桃在快速繁殖中存在的丛生芽诱导率低、增殖率低、生根难等问题,为建立高盆樱桃组培快繁体系奠定基础。【方法】以高盆樱桃带芽茎段为外植体,探讨了外植体的灭菌方法、基本培养基类型(MS、1/2 MS和1/4 MS)以及不同外源激素(6-BA、NAA和IBA)对丛生芽诱导、丛生芽增殖、生根等过程的影响。【结果】高盆樱桃茎段在75%乙醇灭菌20 s,0.1%升汞灭菌300 s时,污染率最低。在MS培养基中添加1.2 mg/L 6-BA和0.1 mg/L IBA时,丛生芽诱导率最高(82.22%)。在MS培养基中同时添加0.8 mg/L 6-BA、0.06 mg/L IBA、0.08 mg/L NAA时丛生芽增殖效果最好,增殖率为7.32。1/2 MS+IBA(0.5 mg/L)培养基有利于生根,生根率达92.22%。【结论】高盆樱桃带芽茎段适宜的灭菌方法为75%酒精处理20 s后,再以0.1%升汞处理300 s; 丛生芽诱导的适宜培养基为MS+6-BA(1.2 mg/L)+IBA(0.1 mg/L); 丛生芽增殖的适宜培养基为MS+6-BA(0.8 mg/L)+IBA(0.06 mg/L)+NAA(0.08 mg/L); 适宜高盆樱桃生根的培养基为1/2 MS+IBA(0.5 mg/L)。以体积比为1(河沙):1(腐殖质):2(蛭石)的混合基质为移栽基质时,试管苗移栽成活率可达72.2%。该试验结果可为高盆樱桃的规模化生产提供一定的理论基础和技术支撑。

Abstract

Abstract: 【Object】 The aim of this study was to resolve the problems of low induction rates, low proliferation rate, and the difficulty of plantlet rooting in Cerasus cerasoides var. cerasoides, and to establish a rapid propagation system through selecting the suitable method for disinfection of C. cerasoides var. cerasoides and optimizing culture conditions. 【Methods】 The effects of sterilization methods, types of basic medias(MS, 1/2 MS and 1/4 MS)and the plant hormones(6-BA, NAA and IBA)on single bud, cluster buds and rooting were investigated using stems with axillary buds of Cerasus cerasoides var. cerasoides as explants. 【Results】 When the stem with axillary buds was sterilized with 75% alcohol for 20 s and 0.1% HgCl₂ for 300 s, the contamination rate was the lowest. MS medium supplemented with 1.2 mg/L 6-BA and 0.1 mg/L IBA showed the highest clustered bud induction rate(82.22%). MS medium with 0.8 mg/L 6-BA, 0.06 mg/L IBA and 0.08 mg/L NAA exhibited the highest clustered bud proliferation rate(7.32). Half MS medium with 0.5 mg/L IBA was suitable for plantlet rooting, and the rooting rate was 92.22%.【Conclusions】The optimal sterilization method for stem segments of C. cerasoides var. cerasoides with axillary buds was the use of 75% alcohol for 20 s and 0.1% HgCl₂ for 300 s. The optimal medium for clustered bud induction was MS containing 1.2 mg/L 6-BA and 0.1 mg/L IBA. The optimal medium for proliferation of the clustered buds was MS supplemented with 0.8 mg/L 6-BA, 0.06 mg/L IBA, and 0.08 mg/L NAA, while the optimal rooting medium was 1/2 MS containing 0.5 mg/L IBA. The survive rate was above 72.2% when the plantlets were due to be moved to the nursery. The medium with 25% sand, 25% humus and 50% roseite was effective for rooting. The results of this study can provide a basis for large-scale propagation of C. cerasoides var. cerasoides.

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张帆,叶露,董姬妃,万雪琴,钟宇. 高盆樱桃的组培快繁研究[J]. 南京林业大学学报（自然科学版）. 2018, 42(03): 111-116 https://doi.org/10.3969/j.issn.1000-2006.201706047

ZHANG Fan, YE Lu, DONG Jifei, WAN Xueqin, ZHONG Yu. Study on tissue culture and rapid propagation of Cerasus cerasoides var. cerasoides[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2018, 42(03): 111-116 https://doi.org/10.3969/j.issn.1000-2006.201706047

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