全基因组测序技术研究及其在木本植物中的应用

doi:10.3969/j.issn.1000-2006.201709020

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南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (05): 172-178.doi: 10.3969/j.issn.1000-2006.201709020

全基因组测序技术研究及其在木本植物中的应用

刘海琳,尹佟明^*

南京林业大学,南方现代林业协同创新中心,江苏南京 210037

出版日期:2018-09-15 发布日期:2018-09-15
基金资助:
收稿日期:2017-09-09 修回日期:2018-03-16 基金项目:国家自然科学基金项目(31570662) 第一作者:刘海琳(lindaliu_njfu@163.com),博士。*通信作者:尹佟明(tmyin@njfu.edu.cn),教授,博士。

Progress on the whole genome sequencing and the application in woody plants

LIU Hailin, YIN Tongming^*

Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

Online:2018-09-15 Published:2018-09-15

摘要/Abstract

摘要： 基因组序列是开展遗传研究重要的信息基础,随着测序技术飞速发展至第3代长片段测序方法,测序读长历经从几十到数万个碱基的提升,对进一步提升基因组组装的完整度以及准确性提供了极大的裨益。现已完成了大量植物种全基因组测序工作,其中木本植物有40多个,还有更多树种的全基因组测序正在进行之中。针对各类测序技术的基因组组装及后续分析,研究人员也开发了大量的生物信息学工具。笔者从测序技术、基因组装技术和全基因组测序生物信息学分析等方面,罗列了目前已完成全基因组测序的木本植物,介绍了全基因组测序技术的发展与应用,以及适用于第3代数据基因组组装的生物学分析软件,为林木基因组研究者提供一定的借鉴。

Abstract: Genome sequence provides one of the essential platforms for different aspects of genetics study for a focal organism. With the fast progress in sequencing technology, it has undergone three generations. The length of reads developed from decades of bases to decades of thousand bases, which has been of great benefit to the completeness and accuracy of genome assembly. Numbers of species have been sequenced the whole genome including more than 40 woody plants thus far, and there are many more sequencing projects of tree genome in process. Consequently, different bioinformatics analytical tools have been developed and applied for genome assembly and the subsequent analyses. Here, in the aspects of sequence technologies, method of genome assembling and the analysis by bioinformatics, we briefly listed the sequenced woody plants, reviewed the technical progress and utility of sequencing technologies, and introduced several analytical methods in genome assembling by using the third generation sequencing technology.

中图分类号:

Q948
S722

刘海琳,尹佟明. 全基因组测序技术研究及其在木本植物中的应用[J]. 南京林业大学学报（自然科学版）, 2018, 42(05): 172-178.

LIU Hailin, YIN Tongming. Progress on the whole genome sequencing and the application in woody plants[J].Journal of Nanjing Forestry University (Natural Science Edition), 2018, 42(05): 172-178.DOI: 10.3969/j.issn.1000-2006.201709020.

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全基因组测序技术研究及其在木本植物中的应用

Progress on the whole genome sequencing and the application in woody plants

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