不同产地银杏黄酮及相关活性物质含量变异分析

吴雅琼; 国靖; 周琦; 胥猛; 汪贵斌; 徐立安

doi:10.3969/j.issn.1000-2006.201803004

吴雅琼,国靖,周琦,胥猛,汪贵斌,徐立安

南京林业大学学报（自然科学版） ›› 2019, Vol. 43 ›› Issue (03) : 183-188.

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南京林业大学学报（自然科学版） ›› 2019, Vol. 43 ›› Issue (03) : 183-188. DOI: 10.3969/j.issn.1000-2006.201803004

研究简报

不同产地银杏黄酮及相关活性物质含量变异分析

吴雅琼,国靖,周琦,胥猛,汪贵斌,徐立安^*

作者信息 +

Variation analysis of flavonoids and related active substances content in ginkgo leaves from different regions

WU Yaqiong, GUO Jing, ZHOU Qi, XU Meng, WANG Guibin, XU Li’an^*

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文章历史 +

摘要

【目的】比较研究来自10个产地的银杏古树叶黄酮,以及与黄酮合成相关的可溶性糖、蛋白质含量和苯丙氨酸解氨酶(PAL)活性,以了解不同产地银杏叶片的活性物质含量变异以及各活性物质间的相关关系。【方法】以10个产地银杏古树叶为材料,测定各活性物质的含量,探究各成分在不同产地间及产地内的变异。【结果】嫁接在相同环境条件下的银杏,其叶片活性物质含量在产地间及产地内均存在显著差异。10个产地银杏古树总黄酮、可溶性糖、蛋白质含量和PAL活性(鲜质量)分别为9.13 mg/g、157.15 mg/g、0.71 g/L和10.96 U/g。银杏叶黄酮含量与可溶性糖含量及PAL活性呈极显著正相关,而可溶性糖和蛋白质含量存在极显著负相关关系。【结论】来自汉中产地的黄酮含量最高,在今后优良叶用银杏来源的选择中应予重视。都匀产地黄酮变量的变异系数最大,在产地间选择的同时也需要关注产地内的选择。

Abstract

【Objective】We aimed to understand the correlation and the variation in the content of active compounds [flavonoids, soluble sugar, protein and phenylalanine ammonia-lyase(PAL)] of Ginkgo biloba L.(ginkgo)leaves from 10 different regions.【Method】Their active compounds determined, geographic sources and individual variations were analyzed.【Result】Under the same environmental conditions, the bioactive contents of ginkgo leaves differed significantly among the 10 regions. The average flavonoids, soluble sugar, protein content and PAL activity of plants from the 10 ginkgo-growing regions were 9.13 mg/g, 157.15 mg/g, 0.71 g/L and 10.96 U/g, respectively. Ginkgo flavonoid content was positively correlated with soluble sugar conten and PAL activity, whereas soluble sugar and protein content were significantly negatively correlated.【Conclusion】Ginkgo from the Hanzhong region had the highest flavonoid content, which should be noted for future sourcing of ginkgo. The variation coefficients for flavonoids in the Duyun region was the largest; thus, more attention must be paid to individual selection from among and within regions.

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吴雅琼,国靖,周琦,胥猛,汪贵斌,徐立安. 不同产地银杏黄酮及相关活性物质含量变异分析[J]. 南京林业大学学报（自然科学版）. 2019, 43(03): 183-188 https://doi.org/10.3969/j.issn.1000-2006.201803004

WU Yaqiong, GUO Jing, ZHOU Qi, XU Meng, WANG Guibin, XU Li’an. Variation analysis of flavonoids and related active substances content in ginkgo leaves from different regions[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2019, 43(03): 183-188 https://doi.org/10.3969/j.issn.1000-2006.201803004

中图分类号： S792.95

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基金

收稿日期:2018-03-02 修回日期:2018-07-06
基金项目:国家林业公益性行业科研专项项目(201504105); 江苏省研究生科研与实践创新计划项目(KYCX18_0954); 江苏高校优势学科建设工程资助项目(PAPD)。
第一作者:吴雅琼(347470439@qq.com),博士生。*通信作者:徐立安(laxu@njfu.edu.cn),教授,ORCID(0000-0001-6616-9747)。