干旱和复水对4个芍药品种生理指标的影响及品种抗旱性评价

doi:10.3969/j.issn.1000-2006.201803030

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2018, Vol. 61 ›› Issue (06): 44-50.doi: 10.3969/j.issn.1000-2006.201803030

干旱和复水对4个芍药品种生理指标的影响及品种抗旱性评价

常青山¹,张利霞^2*,王建章³,王梓¹,徐少君¹,康璐¹,严俊驿¹,杨梦浩¹,赵一凡¹, 刘杨¹

(1.河南科技大学林学院,河南洛阳 471023; 2.河南科技大学农学院,河南洛阳 471023; 3.洛阳国际牡丹园,河南洛阳 471011)

出版日期:2018-11-30 发布日期:2018-11-30
基金资助:
收稿日期:2018-03-19 修回日期:2018-07-17
基金项目:河南省高等学校重点科研资助项目(16A220005,15A180037); 河南省自然科学基金项目(182300410082); 河南省科技攻关计划项目(162102110095); 河南科技大学高级别项目培育基金项目(2015GJB029); 河南科技大学大学生研究训练计划(SRTP)项目(2016154,2017335)
第一作者:常青山(hkdcqs@126.com),讲师,博士。*通信作者:张利霞(hkdzlx@126.com),讲师,博士。

Effects of drought stress and rewatering on physiological indexes of four Paeonia lactiflora cultivars and evaluation of their drought resistance

WANG Zi¹, XU Shaojun¹, KANG Lu¹, YAN Junyi¹, YANG Menghao¹, ZHAO Yifan¹, LIU Yang¹

(1. College of Forestry, Henan University of Science and Technology, Luoyang 471023, China; 2. College of Agriculture, Henan University of Science and Technology, Luoyang 471023, China; 3. Luoyang International Peony Garden,Luoyang 471011, China)

Online:2018-11-30 Published:2018-11-30

摘要/Abstract

摘要： 【目的】通过盆栽实验,选取‘粉玉奴’、‘种生粉’、‘赵园粉’和‘晚霞红’4个芍药品种,研究逐渐干旱及复水条件对4个芍药品种生理指标的影响,以期为芍药抗旱品种的筛选及栽培种植提供依据。【方法】以4种芍药品种为材料,采用自然干旱及复水的方法,于干旱胁迫0、7、14、21 d及复水7 d后测定芍药叶片的生理指标,并进行抗旱性评价,筛选影响品种抗旱性的主要指标。【结果】随着干旱胁迫时间的延长,芍药幼苗叶片相对含水量(RWC)、净光合速率(P_n)、气孔导度(G_s)与蒸腾速率(T_r)呈下降趋势; 而电导率(REC)、丙二醛(MDA)含量、可溶性糖含量(SSC)呈现逐渐增加的趋势,气孔限制值(L_s)呈现先上升后下降的趋势,胞间二氧化碳浓度(C_i)与叶绿素含量(Chl)表现为先降低后升高的趋势; 复水后RWC、C_i与L_s均恢复到对照水平,其他指标则表现出不同程度的恢复。叶绿素含量在干旱胁迫条件下出现了叶绿素“浓缩”的现象,而在复水条件下则恢复正常。主成分分析表明,2个主成分可以代表芍药抗旱性91.459%的原始数据信息量,根据计算出的4个芍药品种的综合评价值(D),对4个芍药品种抗旱性进行了评价; 根据权重对各指标在抗旱中的重要性进行了评估。【结论】根据主成分分析结果,4个芍药抗旱性强弱依次为:‘粉玉奴’>‘种生粉’>‘赵园粉’>‘晚霞红’。依据各指标的权重大小,权重值较高的7个指标(P_n、T_r、G_s、SSC、REC、MDA和RWC)可以作为芍药抗旱性评价的主要指标。

Abstract: 【Objective】 Study the effects of gradual drought and rewatering on the physiological indexes of four herbaceous peony cultivars, and provide a scientific basis for screening drought-resistant varieties of herbaceous peony. 【Method】 Four Paeonia lactiflora(herbaceous peony)cultivars were used as experimental materials, and the physiolo-gical changes in herbaceous peony seedlings were analyzed after drought stress for 0, 7, 14 or 21 d and rewatering for 7 d in pot experiments. The drought resistance of the four herbaceous peony cultivars was comprehensively evaluated, and the major indexes were selected using principal component analysis. 【Result】 With the extension of drought stress time, leaf relative water content(RWC), net photosynthetic rate(P_n), stomatal conductance(G_s), and transpiration rate(T_r)decreased; relative electrical conductivity(REC), malondialdehyde(MDA)content, and soluble sugar content(SSC)increased; stomatal limitation(L_s)increased and then decreased; and intercellular carbon dioxide(C_i)and chlorophyll(Chl)content initially decreased and then increased. After rewatering, RWC, C_i, and L_s were restored to the control level, and the other parameters recovered to some degree. Chlorophyll content was concentrated under drought stress and returned to normal under rehydration conditions. Principal component analysis showed that two principal components could explain 91.459% of the data on herbaceous peony cultivar drought resistance. The drought resistance of the four herbaceous peony cultivars was evaluated according to the comprehensive evaluation value(D), and the importance of each index in drought resistance was evaluated according to the weight of each index. 【Conclusion】 Based on the result of principal component analysis, the drought resistance of the four herbaceous peony cultivars were determined as: ‘Fen Yu Nu’>‘Zhong Sheng Fen’>‘Zhao Yuan Fen’>‘Wan Xia Hong’. Based on the weight of each index, seven indexes with relatively high weights(P_n, T_r, G_s, SSC, REC, MDA and RWC)could be used as the major indexes of drought resistance of herbaceous peony.

中图分类号:

S718.43

常青山,张利霞,王建章,等. 干旱和复水对4个芍药品种生理指标的影响及品种抗旱性评价[J]. 南京林业大学学报（自然科学版）, 2018, 61(06): 44-50.

WANG Zi, XU Shaojun, KANG Lu, YAN Junyi, YANG Menghao, ZHAO Yifan, LIU Yang. Effects of drought stress and rewatering on physiological indexes of four Paeonia lactiflora cultivars and evaluation of their drought resistance[J].Journal of Nanjing Forestry University (Natural Science Edition), 2018, 61(06): 44-50.DOI: 10.3969/j.issn.1000-2006.201803030.

参考文献

[1] 贾清华,刘爱青,刘燕. 不同栽培方式下芍药生长开花及生物量变化[J]. 西南农业学报, 2013, 26(2): 728-732. DOI: 10.16213/j.cnki.scjas.2013.02.072.
JIA Q H, LIU A Q, LIU Y. Growth, florescence and biomass changes of Paeonia lactiflora under different cultivation patterns[J]. Southwest China Journal of Agricultural Sciences, 2013, 26(2): 728-732.
[2] 郭慧. 土壤干旱胁迫对4个芍药品种生理生化反应的影响[D]. 长沙: 湖南农业大学, 2009.
GUO H. Effects of soil drought stress on the physiological and biochemical reaction of four cultivars in Paeonia lactiflora[D]. Changsha: Hunan Agricultural University, 2009.
[3] 王琪,刘建鑫,张建军,等. 水分胁迫对芍药生长和生理生化特性影响的研究[J]. 植物遗传资源学报, 2014, 15(6): 1270-1277.DOI: 10.13430 / j.cnki.jpgr.2014.06.015.
WANG Q, LIU J X, ZHANG J J, et al. The effects of growth and physiology of herbaceous peony under water stress[J]. Journal of Plant Genetic Resources, 2014, 15(6): 1270-1277.
[4] XU Z Z, ZHOU G S, SHIMIZU H. Plant responses to drought and rewatering[J]. Plant Signaling & Behavior, 2010, 5(6): 649-654. DOI: 10.4161/psb.5.6.11398.
[5] 杨文权,顾沐宇,寇建村,等. 干旱及复水对小冠花光合及叶绿素荧光参数的影响[J]. 草地学报, 2013, 21(6): 1130-1135. DOI: 10.11733/j.issn.1007-0435.2013. 06. 015.
YANG W Q, GU M Y, KOU J C, et al. Effects of drought and rewatering on the photosynthesis and chlorophyll fluorescence of Coronilla varia[J]. Acta Agrestia Sinica,2013,21(6): 1130-1135.
[6] 崔婷茹,于慧敏,李会彬,等. 干旱胁迫及复水对狼尾草幼苗生理特性的影响[J]. 草业科学, 2017, 34(4): 788-793. DOI: 10.11829/j.issn.1001-0629.2016-0535.
CUI T R, YU H M, LI H B, et al. Effect of drought stress and rewatering on physiological characteristics of Pennisetum alopecuroides seedlings[J]. Pratacultura Science,2017,34(4): 788-793.
[7] 孔祥生,易现峰. 植物生理学实验原量与技术[M]. 北京: 中国农业出版社, 2012.
[8] 刘杜玲,彭少兵,孙红梅,等. 早实核桃不同品种抗旱性综合评价[J]. 园艺学报, 2014, 42(3):545-553. DOI: 10.16420/j.issn.0513-353x.2014-0917.
LI D L, PENG S B, SUN H M, et al. Comprehensive evaluation on drought resistance of early fruiting walnut cultivars[J]. Acta Horticulturae Sinica, 2014, 42(3):545-553.
[9] 刘新华. 因子分析中数据正向化处理的必要性及其软件实现[J]. 重庆工学院学报(自然科学版), 2009, 23(9): 152-155.
LIU X H. Necessity and software operation of positive management in factor analysis[J].Journal of Chongqing Institute of Technology(Natural Science Edition), 2009, 23(9): 152-155.
[10] 张军,刘红,李晓萍,等. 干旱对小麦孕穗期叶片生理特性及产量的影响[J]. 干旱地区农业研究, 2014, 32(3): 1-8.
ZHANG J, LIU H, LI X P, et al. Effect of drought on leaf phy-siological parameters and yield of wheat at booting stage[J]. Agricultural Research in the Arid Areas, 2014, 32(3): 1-8.
[11] 熊洁,邹小云,陈伦林,等. 油菜苗期耐铝基因型筛选和鉴定指标的研究[J]. 中国农业科学, 2015, 48(16): 3112-3120. DOI: 10.3864/j.issn.0578-1752.2015.16.002.
XIONG J, ZOU X Y, CHEN L L, et al. Screening of rapeseed genotypes with aluminum tolerance at seedling stage and evaluation of selecting indices[J]. Scientia Agricultura Sinica, 2015, 48(16): 3112-3120.
[12] 朱宗河,郑文寅,张学昆. 甘蓝型油菜耐旱相关性状的主成分分析及综合评价[J]. 中国农业科学, 2011, 44(9): 1775-1787. DOI: 10.3864/j.issn.0578-1752.2011.09.003.
ZHU Z H, ZHENG W Y, ZHANG X K. Principal component analysis and comprehensive evaluation on morphological and agronomic traits of drought tolerance in rapeseed(Brassica napus L.)[J]. Scientia Agricultura Sinica, 2011, 44(9): 1775-1787.
[13] 赵雪,张秀珍,牟洪香,等. 干旱胁迫对不同种源文冠果幼苗水分生理特性及渗透调节物质的影响[J]. 东北林业大学学报, 2017, 45(6): 17-21. DOI: 10.13759/j.cnki.dlxb.2017.06.004.
ZHAO X, ZHANG X Z, MOU H X, et al. Effect of drought stress on the water physiological characteristics and the osmotic regulation substances of Xanthoceras sorbifollia seedings from different provenances[J]. Journal of Northeast Forestry University, 2017, 45(6): 17-21.
[14] SOHRABI Y, HEIDARI G, WEISANY W. et al. Changes of antioxidative enzymes, lipid peroxidation and chlorophyll content in chickpea types colonized by different Glomus species under drought stress[J]. Symbiosis, 2012, 56(1): 5-18.
[15] 付宝春,薄伟,秦国杰,等. 景天品种对干旱的生理响应及抗旱性综合评价[J]. 分子植物育种, 2017, 15(3): 1096-1103. DOI: 10.13271/j.mpb.015.001096.
FU B C, BO W, QIN G J, et al. Physiological response of drought stress in sedum species and evaluation of drought resistance[J]. Molecular Plant Breeding, 2017, 15(3): 1096-1103.
[16] 常青山,张利霞,陈煜,等. 菊花黄绿叶突变体的光合与类囊体膜光谱[J]. 林业科学, 2013, 49(2): 72-78. DOI: 10.11707/j.1001-7488.20130211.
CHANG Q S, ZHANG L X, CHEN Y, et al. Characteristics of photosynthesis and spectra properties of thylakoid membranes in the yellow-green leaf mutant of chrysanthemum[J]. Scientia Silvae Sinicae, 2013, 49(2): 72-78.
[17] 胡标林,扬平,万勇,等. 东乡野生稻BILs群体苗期抗旱性综合评价及其遗传分析[J]. 植物遗传资源学报, 2013, 14(2): 249-256. DOI: 10.13430/j.cnki.jpgr.2013.02. 022.
HU B L, YANG P, WAN Y, et al. Comprehensive assessment of drought resistance of bils population derived from dongxiang wild rice(Oryza rufipogon Griff.)at seedling stage and its genetic analysis[J]. Journal of Plant Genetic Resources, 2013, 14(2): 249-256.
[18] 汪灿,周棱波,张国兵,等. 酒用糯高粱资源成株期抗旱性鉴定及抗旱指标筛选[J]. 中国农业科学, 2017, 50(8):1388-1402. DOI: 10.3864/j.issn.0578-1752.2017.08. 004.
WANG C, ZHOU L B, ZHANG G B, et al. Drought resistance identification and drought resistance indices screening of liquor-making waxy sorghum resources at adult plant stage[J]. Scientia Agricultura Sinica, 2017, 50(8): 1388-1402.
[19] 潘昕,邱权,李吉跃,等. 干旱胁迫对青藏高原6种植物生理指标的影响[J]. 生态学报, 2014, 34(13): 3558-3567. DOI: 10.13430/j.cnki.jpgr.2013.02.022.
PAN X, QIU Q, LI J Y, et al. Physiological indexes of six plant species from the tibetan plateau under drought stress[J]. Acta Ecologica Sinica, 2014, 34(13): 3558-3567.

干旱和复水对4个芍药品种生理指标的影响及品种抗旱性评价

Effects of drought stress and rewatering on physiological indexes of four Paeonia lactiflora cultivars and evaluation of their drought resistance

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	张银凤, 蔡洪月, 彭金根, 刘学军, 谢利娟, 张华, 王艳梅. 深圳城市公园不同栽植环境对毛棉杜鹃生长的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 197-204.
[2]	崔令军, 刘瑜霞, 林健, 石开明. 盐胁迫下丛枝菌根真菌对桢楠根系生长和激素的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(4): 119-124.
[3]	曾俊,孙慧珍. 超声发射特征归类识别木质部栓塞信息[J]. 南京林业大学学报（自然科学版）, 2018, 61(01): 89-97.
[4]	茹广欣,刘小囡,朱秀红,张龙冲,王鋆瑞,周霜晴. 泡桐黄化突变体生理特性分析[J]. 南京林业大学学报（自然科学版）, 2017, 60(04): 181-185.
[5]	刘龙云,吴彩娥,李婷婷,陈宗勇. 棕榈花苞营养成分分析[J]. 南京林业大学学报（自然科学版）, 2017, 60(03): 193-197.
[6]	戴前莉,李金花,胡建军,卢孟柱,GIUSEPPENervo. 增施铁对镉胁迫下柳树生长及光合生理性能的改善[J]. 南京林业大学学报（自然科学版）, 2017, 60(02): 63-72.
[7]	王景燕,唐海龙,龚伟,胡文,芶国军. 水肥耦合对汉源花椒幼苗生长、养分吸收和肥料利用的影响[J]. 南京林业大学学报（自然科学版）, 2016, 59(03): 33-40.
[8]	甘小洪1,2,丁雨龙1. 毛竹茎竿纤维细胞发育过程中酸性磷酸酶的动态变化[J]. 南京林业大学学报（自然科学版）, 2006, 49(03): 13-18.
[9]	余观夏1,阮锡根1,封维忠1,黄敏仁2. 杨树无性系超冷现象分析[J]. 南京林业大学学报（自然科学版）, 2006, 49(02): 67-70.
[10]	徐锡增1,徐呈祥2. 硅对盐胁迫下枣树根尖离子微域分布的影响[J]. 南京林业大学学报（自然科学版）, 2006, 49(02): 85-88.
[11]	徐呈祥1,2,马艳萍2,徐锡增1,胡恒康2. 盐胁迫下外源硅调节金丝小枣根和叶片膜脂肪酸的研究[J]. 南京林业大学学报（自然科学版）, 2006, 49(02): 89-93.
[12]	宋立奕1,2,方升佐1. 水培青檀幼苗对NaCl胁迫的生理响应[J]. 南京林业大学学报（自然科学版）, 2006, 49(02): 94-98.
[13]	朱万泽1,吴永波2,薛建辉2. 贡嘎山地区黄背栎的光合特性[J]. 南京林业大学学报（自然科学版）, 2006, 49(01): 25-28.
[14]	万劲1,2,石雷1*,张金政1,汤庚国2. 盐胁迫对鸢尾叶片生理指标的影响[J]. 南京林业大学学报（自然科学版）, 2006, 49(01): 57-60.
[15]	倪建中,王伟,郁书君,贺漫媚,张继方,代色平. 不同种源木棉生长及光合特性研究[J]. 南京林业大学学报（自然科学版）, 2015, 58(06): 185-189.