干旱和复水对4个芍药品种生理指标的影响及品种抗旱性评价

doi:10.3969/j.issn.1000-2006.201803030

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南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (06): 44-50.doi: 10.3969/j.issn.1000-2006.201803030

干旱和复水对4个芍药品种生理指标的影响及品种抗旱性评价

常青山¹,张利霞^2*,王建章³,王梓¹,徐少君¹,康璐¹,严俊驿¹,杨梦浩¹,赵一凡¹, 刘杨¹

(1.河南科技大学林学院,河南洛阳 471023; 2.河南科技大学农学院,河南洛阳 471023; 3.洛阳国际牡丹园,河南洛阳 471011)

出版日期:2018-11-30 发布日期:2018-11-30
基金资助:
收稿日期:2018-03-19 修回日期:2018-07-17
基金项目:河南省高等学校重点科研资助项目(16A220005,15A180037); 河南省自然科学基金项目(182300410082); 河南省科技攻关计划项目(162102110095); 河南科技大学高级别项目培育基金项目(2015GJB029); 河南科技大学大学生研究训练计划(SRTP)项目(2016154,2017335)
第一作者:常青山(hkdcqs@126.com),讲师,博士。*通信作者:张利霞(hkdzlx@126.com),讲师,博士。

Effects of drought stress and rewatering on physiological indexes of four Paeonia lactiflora cultivars and evaluation of their drought resistance

WANG Zi¹, XU Shaojun¹, KANG Lu¹, YAN Junyi¹, YANG Menghao¹, ZHAO Yifan¹, LIU Yang¹

(1. College of Forestry, Henan University of Science and Technology, Luoyang 471023, China; 2. College of Agriculture, Henan University of Science and Technology, Luoyang 471023, China; 3. Luoyang International Peony Garden,Luoyang 471011, China)

Online:2018-11-30 Published:2018-11-30

摘要/Abstract

摘要： 【目的】通过盆栽实验,选取‘粉玉奴’、‘种生粉’、‘赵园粉’和‘晚霞红’4个芍药品种,研究逐渐干旱及复水条件对4个芍药品种生理指标的影响,以期为芍药抗旱品种的筛选及栽培种植提供依据。【方法】以4种芍药品种为材料,采用自然干旱及复水的方法,于干旱胁迫0、7、14、21 d及复水7 d后测定芍药叶片的生理指标,并进行抗旱性评价,筛选影响品种抗旱性的主要指标。【结果】随着干旱胁迫时间的延长,芍药幼苗叶片相对含水量(RWC)、净光合速率(P_n)、气孔导度(G_s)与蒸腾速率(T_r)呈下降趋势; 而电导率(REC)、丙二醛(MDA)含量、可溶性糖含量(SSC)呈现逐渐增加的趋势,气孔限制值(L_s)呈现先上升后下降的趋势,胞间二氧化碳浓度(C_i)与叶绿素含量(Chl)表现为先降低后升高的趋势; 复水后RWC、C_i与L_s均恢复到对照水平,其他指标则表现出不同程度的恢复。叶绿素含量在干旱胁迫条件下出现了叶绿素“浓缩”的现象,而在复水条件下则恢复正常。主成分分析表明,2个主成分可以代表芍药抗旱性91.459%的原始数据信息量,根据计算出的4个芍药品种的综合评价值(D),对4个芍药品种抗旱性进行了评价; 根据权重对各指标在抗旱中的重要性进行了评估。【结论】根据主成分分析结果,4个芍药抗旱性强弱依次为:‘粉玉奴’>‘种生粉’>‘赵园粉’>‘晚霞红’。依据各指标的权重大小,权重值较高的7个指标(P_n、T_r、G_s、SSC、REC、MDA和RWC)可以作为芍药抗旱性评价的主要指标。

Abstract: 【Objective】 Study the effects of gradual drought and rewatering on the physiological indexes of four herbaceous peony cultivars, and provide a scientific basis for screening drought-resistant varieties of herbaceous peony. 【Method】 Four Paeonia lactiflora(herbaceous peony)cultivars were used as experimental materials, and the physiolo-gical changes in herbaceous peony seedlings were analyzed after drought stress for 0, 7, 14 or 21 d and rewatering for 7 d in pot experiments. The drought resistance of the four herbaceous peony cultivars was comprehensively evaluated, and the major indexes were selected using principal component analysis. 【Result】 With the extension of drought stress time, leaf relative water content(RWC), net photosynthetic rate(P_n), stomatal conductance(G_s), and transpiration rate(T_r)decreased; relative electrical conductivity(REC), malondialdehyde(MDA)content, and soluble sugar content(SSC)increased; stomatal limitation(L_s)increased and then decreased; and intercellular carbon dioxide(C_i)and chlorophyll(Chl)content initially decreased and then increased. After rewatering, RWC, C_i, and L_s were restored to the control level, and the other parameters recovered to some degree. Chlorophyll content was concentrated under drought stress and returned to normal under rehydration conditions. Principal component analysis showed that two principal components could explain 91.459% of the data on herbaceous peony cultivar drought resistance. The drought resistance of the four herbaceous peony cultivars was evaluated according to the comprehensive evaluation value(D), and the importance of each index in drought resistance was evaluated according to the weight of each index. 【Conclusion】 Based on the result of principal component analysis, the drought resistance of the four herbaceous peony cultivars were determined as: ‘Fen Yu Nu’>‘Zhong Sheng Fen’>‘Zhao Yuan Fen’>‘Wan Xia Hong’. Based on the weight of each index, seven indexes with relatively high weights(P_n, T_r, G_s, SSC, REC, MDA and RWC)could be used as the major indexes of drought resistance of herbaceous peony.

中图分类号:

S718.43

常青山,张利霞,王建章,等. 干旱和复水对4个芍药品种生理指标的影响及品种抗旱性评价[J]. 南京林业大学学报（自然科学版）, 2018, 42(06): 44-50.

WANG Zi, XU Shaojun, KANG Lu, YAN Junyi, YANG Menghao, ZHAO Yifan, LIU Yang. Effects of drought stress and rewatering on physiological indexes of four Paeonia lactiflora cultivars and evaluation of their drought resistance[J].Journal of Nanjing Forestry University (Natural Science Edition), 2018, 42(06): 44-50.DOI: 10.3969/j.issn.1000-2006.201803030.

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干旱和复水对4个芍药品种生理指标的影响及品种抗旱性评价

Effects of drought stress and rewatering on physiological indexes of four Paeonia lactiflora cultivars and evaluation of their drought resistance

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