建兰花色形成的成分检测

doi:10.3969/j.issn.1000-2006.201806041

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南京林业大学学报（自然科学版） ›› 2019, Vol. 62 ›› Issue (04): 57-62.doi: 10.3969/j.issn.1000-2006.201806041

建兰花色形成的成分检测

李文建^1,2,3,沈永宝^1,2*,史锋厚²,贾文庆⁴

(1.南京林业大学风景园林学院,江苏南京 210037; 2.南京林业大学,南方现代林业协同创新中心,江苏南京 210037; 3.河南城建学院生命科学与工程学院,河南平顶山 467036; 4.河南科技学院园艺园林学院,河南新乡 453003)

出版日期:2019-07-22 发布日期:2019-07-22
基金资助:
收稿日期:2018-06-29 修回日期:2019-05-07 基金项目:江苏高校优势学科建设工程资助项目(PAPD); 河南省科技攻关(国际合作领域)项目(172102410053)。第一作者:李文建(85590067@qq.com),讲师,博士生。*通信作者:沈永宝(ybshen@njfu.com.cn),教授,ORCID(0000-0002-0440-5086)。

Component detection analysis of floral color formation in Cymbidium ensifolium

LI Wenjian^1,2,3, SHEN Yongbao^1,2*, SHI Fenghou², JIA Wenqing⁴

(1. College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China; 2. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; 3. College of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan 467036, China; 4. School of Horticulture Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China)

Online:2019-07-22 Published:2019-07-22

摘要/Abstract

摘要： 【目的】花色是建兰重要的观赏性状之一。对建兰花色形成的色素类成分进行检测分析,为探索建兰花色形成的物质结构组成及新花色品种培育提供依据。【方法】以建兰复色花瓣为材料,用色差计测定花色表型并进行色度比色,检测两种颜色花瓣的总蛋白含量和pH; 通过高效液相色谱-质谱联用,检测两色组织的主要色素成分和结构组成。【结果】黄绿色花瓣组织亮度值(L*, 70.1±1.7)是红色系花瓣组织的近2倍; 红色花瓣红度值(a*, 51.2±1.9)为黄绿色组织4倍多,而黄绿色花瓣黄度值(b*, 35.2±1.5)为红色组织近3倍,两种颜色组织色度差异较显著; 红色系花瓣组织可溶性蛋白含量[(3.44±0.11)mg/g]是黄绿色花瓣组织2倍多; 从两种颜色组织材料中共检测出12种黄酮醇苷和8种花青素苷。【结论】异鼠李素糖苷为建兰黄绿色花瓣组织特有,天竺葵素-二糖苷、飞燕草葡萄糖苷、山奈酚鼠李糖苷、矢车菊素芸香糖苷、山奈酚芸香糖苷和山奈酚芸香糖苷半乳糖苷为红色花瓣组织特有。

Abstract: 【Objective】The flower color is the important ornamental character of Cymbidium ensifolium. In this study, components of C. ensifolium flowers were detected and provided foundation for color composition analysis and new variety cultivation. 【Method】 Firstly, the yellow-green and red petal tissues of six plants were used in this study. Secondly, the phenotypes and digital color matching were detected, and the protein content of petals and pH value were also measured. Finally, the pigment and structural composition in two flower tissues were detected by UV-Vis spectrophotometer and high performance liquid chromatography-mass spectrometry(LC-MS). 【Result】 The yellow-green petals in brightness value(L*, 70.1±1.7)was almost twice than that in red petals. The red petals in red value(a*, 51.2±1.9)was four times more than that of yellow-green petals. The yellow-green petals in yellow value(b*, 35.2±1.5)was about three times more than that of red petals. The result showed conspicuous chromaticity difference between two color petals; The red petals in protein content(3.44±0.11)mg/g was about 2 times more than that in yellow-green petals; twelve flavonol glycosides and 8 anthocyanin were inspected in these sample petals. 【Conclution】Isorhamnetin glycosides is located only in yellow-green petals, and some of which indwell only in the red petals, such as geranium-diglucoside, delphinium glucoside, kaempferol rhamnoside, cornflower rutin glycoside, kaempferol rutinoside and kaempferol rutinoside galactoside.

中图分类号:

S682

李文建,沈永宝,史锋厚,等. 建兰花色形成的成分检测[J]. 南京林业大学学报（自然科学版）, 2019, 62(04): 57-62.

LI Wenjian, SHEN Yongbao, SHI Fenghou, JIA Wenqing. Component detection analysis of floral color formation in Cymbidium ensifolium[J].Journal of Nanjing Forestry University (Natural Science Edition), 2019, 62(04): 57-62.DOI: 10.3969/j.issn.1000-2006.201806041.

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建兰花色形成的成分检测

Component detection analysis of floral color formation in Cymbidium ensifolium

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