白桦BpTCP8基因生物信息学及表达特性分析

doi:10.3969/j.issn.1000-2006.201811066

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南京林业大学学报（自然科学版） ›› 2019, Vol. 43 ›› Issue (5): 67-73.doi: 10.3969/j.issn.1000-2006.201811066

白桦BpTCP8基因生物信息学及表达特性分析

安琳君(), 栾嘉豫, 任丽, 李慧玉, 夏德安^*()

东北林业大学林学院,黑龙江哈尔滨 150040

收稿日期:2018-11-30 修回日期:2019-05-14 出版日期:2019-10-08 发布日期:2019-10-08
通讯作者: 夏德安
基金资助:
国家科技重大专项和重点研发项目省级资金资助项目(GX18B027);国家重点研发计划(2017YFD0600603)

Bioinformatics and expression characteristics analysis of BpTCP8 in Betula platyphylla Suk.

AN Linjun(), LUAN Jiayu, REN Li, LI Huiyu, XIA De’an^*()

School of Forestry, Northeast Forestry University, Harbin 150040, China

Received:2018-11-30 Revised:2019-05-14 Online:2019-10-08 Published:2019-10-08
Contact: XIA De’an

摘要/Abstract

摘要：

【目的】通过研究白桦BpTCP8基因的序列特征及其在不同组织部位、激素处理与胁迫应答中的表达特性,为揭示BpTCP8在白桦生长发育中的功能提供依据。【方法】利用生物信息学方法对BpTCP8基因进行分析,采用实时荧光定量 PCR 技术分析BpTCP8基因在不同组织部位、激素处理与胁迫应答中的表达特性。【结果】生物信息学分析发现,BpTCP8含有TCP家族高度保守的bHLH结构域;qRT-PCR分析结果表明,白桦BpTCP8在发育和衰老初期的叶片中表达量高,并在深裂型叶片、顶芽、木质部、韧皮部中都上调表达。在激素(GA₃、ME-JA、ABA)与非生物胁迫(PEG、NaCl、CdCl₂、NaHCO₃)处理下,BpTCP8都对其产生了相应的应答。【结论】BpTCP8基因可能参与到了白桦叶片成熟、叶型、顶芽、木质部和韧皮部的生长过程中;该基因在GA₃、JA处理中呈现正调控的应答模式,并且可能通过ABA信号途径影响植物抗旱,耐盐、碱、重金属胁迫等。

关键词: 白桦, BpTCP8基因, 表达特性, 激素应答, 胁迫应答

Abstract:

【Objective】 To reveal the function of BpTCP8 in the growth and development ofBetula platyphylla Suk., we analyzed the sequence characteristics, expression pattern in different tissues and the BpTCP8 gene response to hormones and stress.【Method】 The sequence characteristics of BpTCP8 gene were analyzed using bioinformatic methods. Besides, the expression characteristics of the BpTCP8 gene in different tissues as well as its response to hormones and stress were analyzed by real-time fluorescent quantitative PCR.【Result】 Using bioinformatic tools, it was found that the predicted amino acid sequence for the BpTCP8 gene contained a highly conserved bHLH domain, similar to that of the TCP family of transcriptional regulators. Results from qRT-PCR analyses indicated that BpTCP8 was highly expressed during the early developmental stages and during leaf senescence; BpTCP8 was also found to be upregulated in leaves with deep-splitting, apical buds, the xylem and the phloem. Different expression patterns of BpTCP8 were exhibited under different hormone treatments (GA₃, ME-JA and ABA) and abiotic stresses (high PEG, NaCl, CdCl₂ and NaHCO₃concentrations).【Conclusion】 Therefore, the BpTCP8 gene may be involved in leaf maturation and leaf shape, and in the growth of apical buds, the xylem, and the phloem of B. platyphylla Suk.. The gene is positively regulated by GA₃ and JA. BpTCP8 expression may also affect plant resistance to drought, salt, alkali and heavy metals through the ABA signaling pathway.

Key words: Betula platyphylla Suk., BpTCP8 gene, expression characteristics, hormone response, stress response

中图分类号:

S722

安琳君,栾嘉豫,任丽,等. 白桦BpTCP8基因生物信息学及表达特性分析[J]. 南京林业大学学报（自然科学版）, 2019, 43(5): 67-73.

AN Linjun, LUAN Jiayu, REN Li, LI Huiyu, XIA De’an. Bioinformatics and expression characteristics analysis of BpTCP8 in Betula platyphylla Suk.[J].Journal of Nanjing Forestry University (Natural Science Edition), 2019, 43(5): 67-73.DOI: 10.3969/j.issn.1000-2006.201811066.

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引物 perimer	正向引物 forward perimer	反向引物 reverse perimer
BpTCP8	5'-ATGATATCAAATTCAAGAGAA-3'	5'-TTAAGAACCGGTGTTCTCCT-3'
定量PCR引物 qRT-PCR perimer	5'-GCTTGATGCCACTAAGCTTGATAT-3'	5'-CCATGGAATTGTTCAGCAAGG-3'
α-Tubulin	5'-GCACTGGCCTCCAAGGAT-3'	5'-TGGGTCGCTCAATGTCAAGG-3'

白桦BpTCP8基因生物信息学及表达特性分析

Bioinformatics and expression characteristics analysis of BpTCP8 in Betula platyphylla Suk.

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