光质对不同家系青钱柳叶酚类物质积累及抗氧化活性的影响

doi:10.3969/j.issn.1000-2006.201901015

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2020, Vol. 44 ›› Issue (2): 17-25.doi: 10.3969/j.issn.1000-2006.201901015

所属专题：青钱柳叶有效成分专题

• 专题报道(执行主编李维林) • 上一篇下一篇

光质对不同家系青钱柳叶酚类物质积累及抗氧化活性的影响

陈培¹(), 周明明¹, 方升佐¹^,²^,^*(), 刘洋¹, 杨万霞¹^,², 尚旭岚¹^,²

1.南京林业大学林学院,江苏南京 210037
2.南京林业大学,南方现代林业协同创新中心,江苏南京 210037

收稿日期:2019-01-10 修回日期:2019-05-25 出版日期:2020-03-30 发布日期:2020-04-01
通讯作者: 方升佐
基金资助:
国家自然科学基金项目(31470637);江苏省第4期“333工程”培养资金资助项目(BRA2015318)

Effects of light quality on accumulation of phenolic compounds and antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves from different families

CHEN Pei¹(), ZHOU Mingming¹, FANG Shengzuo¹^,²^,^*(), LIU Yang¹, YANG Wanxia¹^,², SHANG Xulan¹^,²

1. College of Forestry, Nanjing Forestry University, Nanjing 210037, China
2. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China

Received:2019-01-10 Revised:2019-05-25 Online:2020-03-30 Published:2020-04-01
Contact: FANG Shengzuo

摘要/Abstract

摘要：

【目的】光质对植物体内酚类物质的积累有着重要的影响。青钱柳作为我国特有的单种属乔木,功能多样,具有材用、药用、观赏价值。通过研究光质处理对不同家系青钱柳叶酚类物质积累及抗氧化活性的影响,为青钱柳苗期培育过程中的环境选择和进一步开发保健产品提供理论依据。【方法】在人工气候室内可控温湿度的条件下对不同家系(广西金钟山7号、广西金钟山6号、四川沐川31号、浙江安吉1号)青钱柳当年生实生苗进行白光、蓝光、绿光和红光4种光质处理,测定其叶片生物量、酚类物质含量及单株产量和体外抗氧化活性[1,1-二苯基-2-三硝基苯肼(DPPH)、自由基清除能力和铁离子还原能力(FRAP)],并分析了抗氧化活性与酚类物质含量的相关性。【结果】除家系对总黄酮含量和单株产量无显著影响外,光质和家系及其交互作用对叶生物量、酚类物质含量和单株产量及抗氧化活性均有显著影响。总体来讲,白光处理下的青钱柳叶生物量最大,酚类物质含量、单株产量及抗氧化活性却最低;蓝光和绿光处理下的青钱柳叶具有较高的酚类含量以及抗氧化活性,但绿光处理下的叶生物量最小。因此,蓝光处理对青钱柳叶酚类物质的积累及其抗氧化能力的提高更有利。从家系层面看,安吉1号家系青钱柳的叶生物量最大,而金钟山6号家系青钱柳的叶酚类含量、酚类单株产量及抗氧化活性最高。相关性分析表明,青钱柳叶总酚及酚酸类物质含量与抗氧化活性呈显著相关(P<0.05)。【结论】光质的调控显著影响不同家系青钱柳叶酚类物质积累和抗氧化活性,蓝光处理下的金钟山6号青钱柳叶的酚类物质积累多且抗氧化能力强。研究结果可为青钱柳保健品的开发及人工林定向培育过程中的光照管理提供参考。

关键词: 青钱柳, 光质, 家系, 酚类, 抗氧化活性, 蓝光

Abstract:

【Objective】Light quality has a certain effect on the accumulation of phenolic compounds in plants. Cyclocarya paliurus (Batal.) Iljinskaja is a unique species in China, it is widely used as a material for wood, medicines and ornamentation. The objective of the present study was to investigate the effect of light quality on the accumulation of phenolic compounds in C. paliurus (Batal.) Iljinskaja leaves from different families and their antioxidant activity and thereby provide a basis for optimizing the light environment during seedling development and further development ofC. paliurus (Batal.) Iljinskaja healthcare products.【Method】Current-year seedlings of C. paliurus (Batal.) Iljinskaja from four families (Guangxi Jinzhongshan No. 7, Guangxi Jinzhongshan No. 6, Sichuan Muchuan No. 31 and Zhejiang Anji No. 1) were treated with four light quality groups (white light, blue light, green light and red light) with controlled temperature and humidity in an artificial climate chamber. Leaf biomass, phenolic content and yield in C. paliurus (Batal.) Iljinskaja leaves as well as phenolic antioxidant activity [DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical-scavenging ability and FRAP (ferric reducing power assay)]in vitro were measured, and the correlation between phenolic content and antioxidant activity was analyzed.【Result】Light quality and family and their interaction had significant effects on leaf biomass, phenolic content and yield per plant and antioxidant activity. However, there were no significant effects of family on the total flavonoid content and yield per plant. In general, white light treatment resulted in the highest value of leaf biomass but the lowest value of phenolic content and yield per plant and antioxidant activity. The seedlings treated with blue light and green light showed higher content of phenolics and antioxidant activity, but green light treatment resulted in the lowest value of leaf biomass. Therefore, blue light treatment was more beneficial in improving the accumulation of phenolics and their antioxidant capacity inC. paliurus (Batal.) Iljinskaja leaves. At the family’s level, the leaf biomass ofC. paliurus (Batal.) Iljinskaja from Anji No. 1 was the highest. However, the highest values of phenolic content and yield per plant and their antioxidant capacity were observed in Jinzhongshan No. 6. The correlation analysis showed that the content of total phenolics and phenolic acids significantly correlated with the antioxidant activity ( P < 0.05). 【Conclusion】Regulation of light quality significantly affected the accumulation of phenolics and their antioxidant activity in the leaves of C. paliurus (Batal.) Iljinskaja belonging to the different families. Leaves of C. paliurus (Batal.) Iljinskaja from Jinzhongshan No. 6 treated with blue light accumulated more phenolics and thereby exhibited higher antioxidant ability. The results provide a reference for the development of C. paliurus (Batal.) Iljinskaja healthcare products and light management for directional cultivation in C. paliurus (Batal.) Iljinskaja plantations.

Key words: Cyclocarya paliurus (Batal.) Iljinskaja, light quality, family, phenolics, antioxidant activities, blue light

中图分类号:

S792.99
S718

陈培,周明明,方升佐,等. 光质对不同家系青钱柳叶酚类物质积累及抗氧化活性的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(2): 17-25.

CHEN Pei, ZHOU Mingming, FANG Shengzuo, LIU Yang, YANG Wanxia, SHANG Xulan. Effects of light quality on accumulation of phenolic compounds and antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves from different families[J].Journal of Nanjing Forestry University (Natural Science Edition), 2020, 44(2): 17-25.DOI: 10.3969/j.issn.1000-2006.201901015.

图/表 6

表1

图1

图2

图3

图4

表2

参考文献 32

[1]	谢明勇, 谢建华. 青钱柳研究进展[J]. 食品与生物技术学报, 2008,27(1):113-121.
	XIE M Y, XIE J H. Review about the research on Cyclocarya paliurus (Batal.) Iljinskaja [J]. Journal of Food Science and Biotechnology, 2008,27(1):113-121. DOI: 10.3321/j.issn:1673-1689.2008.01.021.
[2]	中国药材公司. 中国中药资源志要[M]. 北京: 科学出版社, 1994.
	Chinese Herbal Medicine Company. Resources of traditional Chinese medicine[M]. Beijing: Science Press, 1994.
[3]	陈玮玲, 钟培培, 王远兴. 青钱柳叶活性成分的抗氧化活性及UPLC-QTOF-MS/MS分析[J]. 食品科学, 2017,38(8):122-128.
	CHEN W L, ZHONG P P, WANG Y X. Antioxidant activities and identification of bioactive components of Cyclocarya paliurus leaves by UPLC-QTOF-MS/MS [J]. Food Science, 2017,38(8):122-128. DOI: 10.7506/spkx1002-6630-201708020.
[4]	舒任庚, 舒积成. 青钱柳中的酚类化学成分[J]. 中草药, 2007,38(4):507-508.
	SHU R G, SHU J C. The phenolic constituents in Cyclocarya paliurus [J]. Chinese Traditional and Herbal Drugs, 2007,38(4):507-508. DOI: 10.3321/j.issn:0253-2670.2007.04.010.
[5]	LIU Y, QIAN C Y, DING S H, et al. Effect of light regime and provenance on leaf characteristics, growth and flavonoid accumulation in Cyclocarya paliurus (Batal.) Iljinskaja coppices[J]. Botanical Studies, 2016, 57:28. DOI: 10.1186/s40529-016-0145-7. doi: 10.1186/s40529-016-0145-7
[6]	周昕, 汪贵斌, 刘琳, 等. 光强对喜树幼苗生长及喜树碱含量的影响[J]. 南京林业大学学报(自然科学版), 2016,40(3):9-14.
	ZHOU X, WANG G B, LIU L, et al. Effects of light intensity on the growth of Camptotheca acuminata seedlings and camptothecin contents [J]. J Nanjing For Univ (Nat Sci Ed), 2016,40(3):9-14. DOI: 10.3969/j.issn.1000-2006.2016.03.002.
[7]	徐友, 王欢利, 汪贵斌, 等. 温度和光照强度对银杏叶黄酮合成的影响[J]. 中南林业科技大学学报, 2016,36(4):30-34.
	XU Y, WANG H L, WANG G B, et al. Effects of temperature and light intensity on flavonoid biosynjournal of ginkgo (Ginkgo biloba L.) leaves [J]. Journal of Central South University of Forestry & Technology, 2016,36(4):30-34. DOI: 10.14067/j.cnki.1673-923x.2016.04.006.
[8]	QIAN H M, LIU T Y, DENG M D, et al. Effects of light quality on main health-promoting compounds and antioxidant capacity of Chinese kale sprouts[J]. Food Chemistry, 2016, 196:1232-1238. DOI: 10.1016/j.foodchem.2015.10.055. doi: 10.1016/j.foodchem.2015.10.055
[9]	LEE N Y, LEE M J, KIM Y K, et al. Effect of light emitting diode radiation on antioxidant activity of barley leaf[J]. Journal of the Korean Society for Applied Biological Chemistry, 2010, 53(6):685-690. DOI: 10.3839/jksabc.2010.104. doi: 10.3839/jksabc
[10]	李亚华, 陈龙, 高荣广, 等. LED 光质对茄子果实品质及抗氧化能力的影响[J]. 应用生态学报, 2015,26(9):2728-2734.
	LI Y H, CHEN L, GAO R G, et al. Effects of LED qualities on quality and antioxidation capacity of eggplant fruits[J]. Chinese Journal of Applied Ecology, 2015,26(9):2728-2734. DOI: 10.13287/j.1001-9332.20150630.008.
[11]	LIU Y, SONG L L, YU W W, et al. Light quality modifies camptothecin production and gene expression of biosynjournal in Camptotheca acuminata Decne seedlings[J]. Industrial Crops and Products, 2015, 66:137-143. DOI: 10.1016/j.indcrop.2014.12.046. doi: 10.1016/j.indcrop.2014.12.046
[12]	黄开栋, 钱壮壮, 唐罗忠. 林木修枝对林地环境的影响研究进展[J]. 世界林业研究, 2018,31(2):43-48.
	HUANG K D, QIAN Z Z, TANG L Z. Research progress in the effect of pruning on plantation environment[J]. World Forestry Research, 2018,31(2):43-48. DOI: 10.13348/j.cnki.sjlyyj.2017.0088.y.
[13]	陈龙, 景丹龙, 王军辉. 6个不同种源梓树叶片的总酚含量和抗氧化性能的比较[J]. 西北林学院学报, 2015,30(6):222-226,278.
	CHEN L, JING D L, WANG J H. Comparison of total phenols and antioxidant activity in the leaves of Catalpa ovate from six provenances [J]. Journal of Northwest Forestry University, 2015,30(6):222-226, 278. DOI: 10.3969/j.issn.1001-7461.2015.06.40.
[14]	SINGH S, SINGH D R, BANU V S, et al. Functional constituents (micronutrients and phytochemicals) and antioxidant activity of Centella asiatica (L.) Urban leaves[J]. Industrial Crops and Products, 2014, 61:115-119. DOI: 10.1016/j.indcrop.2014.06.045. doi: 10.1016/j.indcrop.2014.06.045
[15]	DENG B, SHANG X L, FANG S Z, et al. Integrated effects of light intensity and fertilization on growth and flavonoid accumulation in Cyclocarya paliurus[J]. Journal of Agricultural and Food Chemistry, 2012, 60(25):6286-6292. DOI: 10.1021/jf301525s. doi: 10.1021/jf301525s
[16]	邓波, 刘桂华, 余云云, 等. 遮荫和种源对青钱柳三萜类化合物积累的影响[J]. 生态学杂志, 2018,37(2):383-390.
	DENG B, LIU G H, YU Y Y, et al. Effects of shading and provenance on triterpenoid accumulation in leaves of Cyclocarya paliurus [J]. Chinese Journal of Ecology, 2018,37(2):383-390. DOI: 10.13292/j.1000-4890.201802.002.
[17]	CAO Y N, FANG S Z, YIN Z Q, et al. Chemical fingerprint and multicomponent quantitative analysis for the quality evaluation of Cyclocarya paliurus leaves by HPLC-Q-TOF-MS[J]. Molecules, 2017, 22(11):1927. DOI: 10.3390/molecules22111927. doi: 10.3390/molecules22111927
[18]	KOSANIĆ M, RANKOVIĆ B, VUKOJEVIĆ J. Antioxidant properties of some lichen species[J]. Journal of Food Science and Technology, 2011, 48(5):584-590. DOI: 10.1007/s13197-010-0174-2. doi: 10.1007/s13197-010-0174-2
[19]	WONG C C, LI H B, CHENG K W, et al. A systematic survey of antioxidant activity of 30 Chinese medicinal plants using the ferric reducing antioxidant power assay[J]. Food Chemistry, 2006, 97(4):705-711. DOI: 10.1016/j.foodchem.2005.05.049. doi: 10.1016/j.foodchem.2005.05.049
[20]	余龙江, 赵春芳. 次生代谢产物生物合成: 原理与应用[M]. 北京: 化学工业出版社, 2017.
	YU L J, ZHAO C F. Biosynthesis of secondary metabolites: theory and application[M]. Beijing: Chemical Industry Press, 2017.
[21]	潘俊倩, 佟曦然, 郭宝林. 光对植物黄酮类化合物的影响研究进展[J]. 中国中药杂志, 2016,41(21):3897-3903.
	PAN J Q, TONG X R, GUO B L. Progress of effects of light on plant flavonoids[J]. China Journal of Chinese Materia Medica, 2016,41(21):3897-3903. DOI: 10.4268/cjcmm20162103.
[22]	EVANS J R. The dependence of quantum yield on wavelength and growth irradiance[J]. Functional Plant Biology, 1987, 14(1):69-79. DOI: 10.1071/pp9870069. doi: 10.1071/PP9870069
[23]	MIRANDA J H, WILLIAMS R. Developmental influence of in vitro light quality and carbon dioxide on photochemical efficiency of PSII of strawberry leaves (Fragaria × ananassa)[J]. Journal of Applied Horticulture, 2007, 9(1):13-16. doi: 10.37855/jah.2007.v09i01.03
[24]	LIU Y, FANG S Z, YANG W X, et al. Light quality affects flavonoid production and related gene expression in Cyclocarya paliurus[J]. Journal of Photochemistry and Photobiology B: Biology, 2018, 179:66-73. DOI: 10.1016/j.jphotobiol.2018.01.002. doi: 10.1016/j.jphotobiol.2018.01.002
[25]	LIU H K, CHEN Y Y, HU T T, et al. The influence of light-emitting diodes on the phenolic compounds and antioxidant activities in pea sprouts[J]. Journal of Functional Foods, 2016, 25:459-465. DOI: 10.1016/j.jff.2016.06.028. doi: 10.1016/j.jff.2016.06.028
[26]	SHIGA T, SHOJI K, SHIMADA H, et al. Effect of light quality on rosmarinic acid content and antioxidant activity of sweet basil, Ocimum basilicum L.[J]. Plant Biotechnology, 2009, 26(2):255-259. DOI: 10.5511/plantbiotechnology.26.255. doi: 10.5511/plantbiotechnology.26.255
[27]	鲁燕舞, 张晓燕, 耿殿祥, 等. 光质对萝卜芽苗菜总酚类物质含量及抗氧化能力的影响[J]. 园艺学报, 2014,41(3):545-552.
	LU Y W, ZHANG X Y, GENG D X, et al. Effects of light quality on total phenolic contents and antioxidant activity in radish sprouts[J]. Acta Horticulturae Sinica, 2014,41(3):545-552. DOI: 10.16420/j.issn.0513-353x.2014.03.016.
[28]	李波, 黄明菊, 李妍岚, 等. 肿节风中咖啡酸衍生物及抗氧化活性[J]. 沈阳药科大学学报, 2009,26(11):900-903, 910.
	LI B, HUANG M J, LI Y L, et al. Antioxidant constituents of Sarcandra glabra (Thunb.)Nakai [J]. Journal of Shenyang Pharmaceutical University, 2009,26(11):900-903, 910. DOI: 10.14066/j.cnki.cn21-1349/r.2009.11.011.
[29]	马天翔, 史宁, 陈乾, 等. 红景天中8 种成分体外抗氧化作用的比较[J]. 中国药理学通报, 2012,28(9):1224-1228.
	MA T X, SHI N, CHEN Q, et al. Comparison on the antioxidant activity of eight components from Rhodiola in vitro [J]. Chinese Pharmacological Bulletin, 2012,28(9):1224-1228. DOI: 10.3969/j.issn.1001-1978.2012.09.010.
[30]	申海进, 郭巧生, 房海灵. 野菊花60%乙醇提取物的酚类成分组成及其清除自由基和防霉变能力分析[J]. 植物资源与环境学报, 2010,19(1):20-25.
	SHEN H J, GUO Q S, FANG H L. Analyses on phenolic compositions, abilities of scavenging free radicals and mildewproof of 60% ethanol extract from Dendranthema indicum flower [J]. Journal of Plant Resources and Environment, 2010,19(1):20-25. DOI: 10.3969/j.issn.1674-7895.2010.01.004.
[31]	林智, 吕海鹏, 崔文锐, 等. 普洱茶的抗氧化酚类化学成分的研究[J]. 茶叶科学, 2006,26(2):112-116.
	LIN Z, LU H P, CUI W R, et al. Study on antioxidative polyphenol compounds in Pu’er tea[J]. Journal of Tea Science, 2006,26(2):112-116. DOI: 10.3969/j.issn.1000-369X.2006.02.007.
[32]	崔琳琳, 周一鸣, 季红斌, 等. 不同光质对苦荞萌发过程中的黄酮类化合物及其抗氧化活性的影响[J]. 食品工业科技, 2016,37(6):104-108.
	CUI L L, ZHOU Y M, JI H B, et al. Effects of light quality on flavonoids and antioxidant properties of germinated tartary buckwheat[J]. Science and Technology of Food Industry, 2016,37(6):104-108. DOI: 10.13386/j.issn1002-0306.2016.06.012.

处理号 treatment No.	光质 light quality	家系 family
WJ7	WL(白色LED)	J7(金钟山7号)
WJ6	WL(白色LED)	J6(金钟山6号)
WM	WL(白色LED)	M(沐川31号)
WA	WL(白色LED)	A(安吉1号)
BJ7	BL(蓝色LED)	J7(金钟山7号)
BJ6	BL(蓝色LED)	J6(金钟山6号)
BM	BL(蓝色LED)	M(沐川31号)
BA	BL(蓝色LED)	A(安吉1号)
GJ7	GL(绿色LED)	J7(金钟山7号)
GJ6	GL(绿色LED)	J6(金钟山6号)
GM	GL(绿色LED)	M(沐川31号)
GA	GL(绿色LED)	A(安吉1号)
RJ7	RL(红色LED)	J7(金钟山7号)
RJ6	RL(红色LED)	J6(金钟山6号)
RM	RL(红色LED)	M(沐川31号)
RA	RL(红色LED)	A(安吉1号)

抗氧化活性 antioxidant activity	EC₅₀	TPC	TF	TPA	PA1	PA2	PA3	F1	F2	F3	F4	F5	F6
IC₅₀	0.810^**	-0.653^**	-0.340	-0.785^**	-0.730^**	-0.773^**	-0.706^**	-0.306	-0.354	-0.325	-0.252	-0.326	-0.265
EC₅₀	—	-0.584^*	-0.195	-0.799^**	-0.768^**	-0.780^**	-0.661^**	-0.178	-0.195	-0.225	-0.131	-0.185	-0.155

光质对不同家系青钱柳叶酚类物质积累及抗氧化活性的影响

Effects of light quality on accumulation of phenolic compounds and antioxidant activities in Cyclocarya paliurus (Batal.) Iljinskaja leaves from different families

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 32

相关文章 15

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	王改萍, 章雷, 曹福亮, 丁延朋, 赵群, 赵慧琴, 王峥. 红蓝光质对银杏苗木生长生理特性及黄酮积累的影响[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 105-112.
[2]	宋子琪, 卞国良, 林峰, 胡凤荣, 尚旭岚. 流式细胞仪鉴定青钱柳倍性方法的建立及其应用[J]. 南京林业大学学报（自然科学版）, 2024, 48(2): 61-68.
[3]	王纪, 方升佐. 不同抗褐化剂对青钱柳愈伤组织酶活性和生长的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(6): 167-174.
[4]	苏泾涵, 王改萍, 刘玉华, 戚亚, 彭大庆, 李守科, 曹福亮. 叶用文冠果总多酚提取工艺及抗氧化活性分析[J]. 南京林业大学学报（自然科学版）, 2023, 47(5): 129-137.
[5]	黄梓良, 徐子恒, 孙操稳. 青钱柳种子雨的季节动态及土壤种子库特征[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 18-26.
[6]	王文月, 张振, 金国庆, 孙林山, 邱勇斌, 周志春, 杨涛. 两地点8年生柏木生长性状家系变异及选择[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 42-48.
[7]	方升佐. 青钱柳产业发展历程及资源培育研究进展[J]. 南京林业大学学报（自然科学版）, 2022, 46(6): 115-126.
[8]	贾展慧, 贾晓东, 许梦洋, 莫正海, 翟敏, 宣继萍, 张计育, 王刚, 王涛, 郭忠仁. 薄壳山核桃原花青素合成关键酶基因的克隆与表达分析[J]. 南京林业大学学报（自然科学版）, 2022, 46(5): 49-57.
[9]	贾庆彬, 刘庚, 赵佳丽, 李奎友, 孙文生. 红松半同胞家系生长性状变异分析与优良家系选择[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 109-116.
[10]	王碧霞, 杜小奇, 邓燕, 蔡晓梅, 苏光灿. 凉山油橄榄主栽品种叶中营养物质和酚类含量的季节性变化[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 169-176.
[11]	李丹丹, 翁启杰, 甘四明, 周长品, 黄世能, 李梅. 基于EST-SSR标记鉴定猴耳环自由授粉的全同胞子代[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 95-101.
[12]	徐展宏, 朱莹, 金慧颖, 孙操稳, 方升佐. 不同叶色青钱柳叶片色素、多酚含量及光合特性的差异[J]. 南京林业大学学报（自然科学版）, 2022, 46(2): 103-110.
[13]	孙操稳, 仲文雯, 洑香香, 尚旭岚, 方升佐. 青钱柳幼林地上部分生物量生长模型研究[J]. 南京林业大学学报（自然科学版）, 2022, 46(1): 138-144.
[14]	李娜, 朱培林, 丰采, 温敏学, 方升佐, 尚旭岚. 青钱柳嫁接愈合过程中砧穗生理特性及其与亲和性的关系[J]. 南京林业大学学报（自然科学版）, 2021, 45(1): 13-20.
[15]	周永晟, 徐子恒, 袁发银, 尚旭岚, 孙操稳, 方升佐. 亚热带3个地点青钱柳群落特征比较[J]. 南京林业大学学报（自然科学版）, 2021, 45(1): 29-35.