新型分子生物学技术在花卉定向育种中的应用进展

doi:10.3969/j.issn.1000-2006.201902014

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南京林业大学学报（自然科学版） ›› 2019, Vol. 43 ›› Issue (6): 173-180.doi: 10.3969/j.issn.1000-2006.201902014

新型分子生物学技术在花卉定向育种中的应用进展

夏溪(), 奉树成, 张春英^*()

上海植物园,上海城市植物资源开发应用工程技术中心,上海 200231

收稿日期:2019-02-19 修回日期:2019-05-08 出版日期:2019-11-30 发布日期:2019-11-30
通讯作者: 张春英
基金资助:
上海市科学技术委员会项目(18DZ2283500)

Advance in flower directive breeding using new molecular biology techniques

XIA Xi(), FENG Shucheng, ZHANG Chunying^*()

Shanghai Botanical Garden,Shanghai Urban Plant Resources Development and Application Engineering Research Center,Shanghai 200231, China

Received:2019-02-19 Revised:2019-05-08 Online:2019-11-30 Published:2019-11-30
Contact: ZHANG Chunying

摘要/Abstract

摘要：

分子生物学技术可以提高花卉定向育种的效率和精准度,其中转基因技术是目前应用最广泛的花卉定向育种技术,基因编辑和高通量测序技术是近年来发展迅速的分子生物学技术。基于国内外研究现状,阐述了转基因和基因编辑技术在花色、花香、株型和抗性育种中的应用,以及高通量测序技术在花卉目标性状调控的分子机制研究、基因定位、分子标记开发方面助力花卉育种的研究进展。提出了利用新型分子生物学技术加快花卉定向育种进程的研究方向与建议,以期为定向培育出具有新奇花色、花香、花型、抗性的观赏植物新品种提供参考。

关键词: 花卉, 定向育种, 转基因技术, 基因编辑, 高通量测序

Abstract:

Molecular biology techniques can be used to improve the precision and efficiency of flower directive breeding. In various molecular breeding techniques, the transgenic is the most widely used one, the gene editing and next generation sequencing (NGS) techniques have been more and more adopted in recent years. In this article, we reviewed the recent flower breeding studies in color, fragrance, plant shape and resistance breeding using transgenic and gene editing. In addition, we introduced how NGS was used in molecular mechanism, gene localization and molecular marker development, which could empower in breeding. Finally, we make the suggestions on how to use new molecular biology techniques in directive breeding, aiming at providing references for the directive breeding of ornamental plants with novel flower colors, fragrance, morphology and stress tolerance.

Key words: flower, directive breeding, transgenic techniques, gene editing, next generation sequencing (NGS)

中图分类号:

夏溪,奉树成,张春英. 新型分子生物学技术在花卉定向育种中的应用进展[J]. 南京林业大学学报（自然科学版）, 2019, 43(6): 173-180.

XIA Xi, FENG Shucheng, ZHANG Chunying. Advance in flower directive breeding using new molecular biology techniques[J].Journal of Nanjing Forestry University (Natural Science Edition), 2019, 43(6): 173-180.DOI: 10.3969/j.issn.1000-2006.201902014.

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新型分子生物学技术在花卉定向育种中的应用进展

Advance in flower directive breeding using new molecular biology techniques

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[1]	王伟, 邱志楠, 李爽, 白向东, 刘桂丰, 姜静. CRISPR/Cas9核糖核蛋白介导的无T-DNA插入的白桦BpGLK1精准突变[J]. 南京林业大学学报（自然科学版）, 2024, 48(1): 11-17.
[2]	陈赢男, 韦素云, 曲冠正, 胡建军, 王军辉, 尹佟明, 潘惠新, 卢孟柱, 康向阳, 李来庚, 黄敏仁, 王明庥. 现代林木育种关键核心技术研究现状与展望[J]. 南京林业大学学报（自然科学版）, 2022, 46(6): 1-9.
[3]	丁晓磊, 张悦, 林司曦, 叶建仁. 基于高通量测序技术的松材线虫研究进展[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 1-7.
[4]	孙佳彤, 国艳娇, 李爽, 周晨光, 姜立泉, 李伟. 基于CRISPR/Cas9的毛果杨bHLH106转录因子的功能研究[J]. 南京林业大学学报（自然科学版）, 2021, 45(6): 15-23.
[5]	陈秀波, 段文标, 陈立新, 朱德全, 赵晨晨, 刘东旭. 小兴安岭3种原始红松混交林土壤nirK型反硝化微生物群落特征[J]. 南京林业大学学报（自然科学版）, 2021, 45(2): 77-86.
[6]	李磊, 蒋敬, 陈云霞. DNA宏条形码技术在动植物法医鉴定中的应用进展[J]. 南京林业大学学报（自然科学版）, 2021, 45(1): 235-241.
[7]	季淮, 韩建刚, 李萍萍, 朱咏莉, 郭俨辉, 郝达平, 崔皓. 洪泽湖湿地植被类型对土壤有机碳粒径分布及微生物群落结构特征的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(1): 141-150.
[8]	黄金思, 奚晓桐, 丁晓磊, 叶建仁. 基于SNP标记的广东省松材线虫种群分化研究[J]. 南京林业大学学报（自然科学版）, 2019, 43(6): 25-31.
[9]	马秋月,戴晓港,李淑娴. 外显子组测序技术述评[J]. 南京林业大学学报（自然科学版）, 2016, 40(04): 157-163.
[10]	李红婷,董然. 铅在4种宿根花卉中的亚细胞分布及迁移转化特点[J]. 南京林业大学学报（自然科学版）, 2015, 39(04): 57-62.
[11]	马秋月,廖卓毅,张得芳,戴晓港,陈赢男,李淑娴. 碧桃花瓣转录组微卫星特征分析[J]. 南京林业大学学报（自然科学版）, 2015, 39(03): 34-38.
[12]	王磊;汤庚国;刘彤;赵九洲. 球根花卉花期调控的研究进展[J]. 南京林业大学学报（自然科学版）, 2004, 28(01): 66-70.
[13]	杜有新;杨涛;李成才;沈延松. 云南高山花卉种质资源调查[J]. 南京林业大学学报（自然科学版）, 2003, 27(05): 80-84.
[14]	蔡友铭;武雯;邹惠渝;黄敏仁. 分子标记在花卉研究中的应用现状[J]. 南京林业大学学报（自然科学版）, 2002, 26(02): 84-86.