
新型分子生物学技术在花卉定向育种中的应用进展
Advance in flower directive breeding using new molecular biology techniques
分子生物学技术可以提高花卉定向育种的效率和精准度,其中转基因技术是目前应用最广泛的花卉定向育种技术,基因编辑和高通量测序技术是近年来发展迅速的分子生物学技术。基于国内外研究现状,阐述了转基因和基因编辑技术在花色、花香、株型和抗性育种中的应用,以及高通量测序技术在花卉目标性状调控的分子机制研究、基因定位、分子标记开发方面助力花卉育种的研究进展。提出了利用新型分子生物学技术加快花卉定向育种进程的研究方向与建议,以期为定向培育出具有新奇花色、花香、花型、抗性的观赏植物新品种提供参考。
Molecular biology techniques can be used to improve the precision and efficiency of flower directive breeding. In various molecular breeding techniques, the transgenic is the most widely used one, the gene editing and next generation sequencing (NGS) techniques have been more and more adopted in recent years. In this article, we reviewed the recent flower breeding studies in color, fragrance, plant shape and resistance breeding using transgenic and gene editing. In addition, we introduced how NGS was used in molecular mechanism, gene localization and molecular marker development, which could empower in breeding. Finally, we make the suggestions on how to use new molecular biology techniques in directive breeding, aiming at providing references for the directive breeding of ornamental plants with novel flower colors, fragrance, morphology and stress tolerance.
花卉 / 定向育种 / 转基因技术 / 基因编辑 / 高通量测序
flower / directive breeding / transgenic techniques / gene editing / next generation sequencing (NGS)
[1] |
|
[2] |
|
[3] |
|
[4] |
|
[5] |
|
[6] |
|
[7] |
|
[8] |
|
[9] |
|
[10] |
|
[11] |
|
[12] |
|
[13] |
|
[14] |
|
[15] |
|
[16] |
|
[17] |
|
[18] |
李明亮, 张辉, 胡建军, 等. 转Bt基因和蛋白酶抑制剂基因杨树抗虫性的研究[J]. 林业科学, 2000, 36(2):93-97. DOI: 10.3321/j.issn:1001-7488.2000.02.015.
|
[19] |
|
[20] |
|
[21] |
|
[22] |
|
[23] |
|
[24] |
洪波, 仝征, 李邱华, 等. 地被菊花Fall Color体细胞胚途径再生、遗传转化及转基因植株的抗寒性检测[J]. 中国农业科学, 2006, 39(7):1443-1450. DOI: 10.1360/aps040087.
|
[25] |
|
[26] |
|
[27] |
|
[28] |
|
[29] |
|
[30] |
|
[31] |
|
[32] |
|
[33] |
潘洪杏, 刘侠, 万秀清, 等. 利用CRISPR-Cas9基因组编辑技术定向敲除烟草eIF4E-6基因[J]. 分子植物育种, 2017, 15(2):538-544. DOI: 10.13271/j.mpb.015.000538.
|
[34] |
|
[35] |
|
[36] |
|
[37] |
|
[38] |
|
[39] |
|
[40] |
|
[41] |
|
[42] |
|
[43] |
|
[44] |
|
[45] |
|
[46] |
|
[47] |
|
[48] |
|
[49] |
|
[50] |
|
[51] |
|
[52] |
张少平, 洪建基, 邱珊莲, 等. 紫背天葵高通量转录组测序分析[J]. 园艺学报, 2016, 43(5):935-946. DOI: 10.16420/j.issn.0513-353x.2016-0140.
|
[53] |
|
[54] |
何新颖, 戚杰, 胡永红. 基于比较转录组分析‘乌龙捧盛’牡丹花瓣初步着色的分子机理[G]//张启翔.中国观赏园艺研究进展. 北京: 中国林业出版社, 2017.
|
[55] |
|
[56] |
姜福星, 魏丕伟, 江欣燕, 等. 白花虎眼万年青高通量测序及转录组分析[J]. 现代园林, 2015, 12(4):312-313.
|
[57] |
|
[58] |
何崇单, 蔡雨蒙, 李萌, 等. 基于银杏花芽3个分化时期转录组测序的相关基因筛选与表达分析[J]. 园艺学报, 2018, 45(8):1479-1490. DOI: 10.16420/j.issn.0513-353x.2018-0023.
|
[59] |
孙霞, 郑成淑, 王秀峰, 等. 菊花花芽分化期叶片和茎尖转录组表达分析[G]//张启翔.中国观赏园艺研究进展. 北京: 中国林业出版社, 2011.
|
[60] |
|
[61] |
肖文芳, 李佐, 陈和明, 等. 基于转录组测序的蝴蝶兰微卫星特征分析[G]//张启翔.中国观赏园艺研究进展. 北京: 中国林业出版社, 2015.
|
[62] |
|
[63] |
|
/
〈 |
|
〉 |