
盐胁迫对大花四照花种子萌发与幼苗生长的影响
Effects of salt stress on seed germination and seedling growth of Cornus florida
探究盐胁迫对大花四照花种子萌发及幼苗生长的影响,为大花四照花在滨海地区的推广提供理论依据。
对大花四照花种子采取两种预处理:T1处理为用清水配制500 mg/L的GA3浸泡3 d,并进行低温层积;T2处理用4种浓度盐溶液(质量分数分别为0.20%、0.30%、0.40%和0.45%)配制的500 mg/L GA3浸泡3 d,低温层积。层积2个月后进行发芽、幼苗形态和生理指标测定。发芽基质(沙)和水分管理:T1处理种子分别采用5个盐浓度水平[0(CK)、0.20%.0.30%.0.40%和0.45%]进行处理,而T2处理种子则采用与预处理相一致的盐浓度水平。
盐胁迫极显著降低了大花四照花种子的发芽率(GR)、发芽势(GE)、发芽指数(GI)和成苗率,并且使其盐害率极显著上升(P<0.01)o相对于T1处理,T2处理对各指标的影响更大,尤其当盐溶液质量分数≥0.3%时,各处理水平的GI在2个处理间差异显著。盐胁迫对幼苗生长指标的影响主要体现在苗高上,其次是根长、相对含水量、鲜质量和干质量。同时,丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和脯氨酸(Pro)含量均随着盐浓度的上升而显著增加,而可溶性糖(SSC)含量在盐溶液质量分数为0.40%时达到最高,然后开始下降;盐胁迫对K+含量的影响显著(P<0.05),且随着盐浓度的增加而下降,但对Na+含量的影响不显著。
盐胁迫抑制了大花四照花种子的发芽,加盐预处理加剧了抑制作用,而且显著地影响了幼苗的生长指标、生理指标和对离子的吸收。综合来看,大花四照花种子和幼苗可在0.30%的盐胁迫条件下正常萌发和生长,因此可作为滨海地区潜在的候选观赏树种,但需要进一步的试验性栽培来验证。
The purpose of this study was to demonstrate the effects of salt stress on seed germination and seedling growth of Corios florida, to provide a basis for its potential application in coastal areas.
The seeds of C.florida in pretreatment 1 (T1) were soaked with 500 mg/L gibberellin acid (GA3) , which was prepared with water. The seeds of pretreatment 2 (T2) were soaked with 500 mg/L gibberellin acid (GA3), which was prepared with four concentrations of salt solution(0.20%, 0.30%, 0.40% and 0.45%) .All the seeds were soaked for three days, followed by moist chilling for two months, then the germination testing, measurements of seedling growth and biochemical indexes were carried out. Germinating substrate (sand) and water management were as follows: five salt concentrations [0(CK), 0.20%, 0.30%, 0.40% and 0.45%] were used in T1 treatment seeds, while the corresponding to pretreatment salt concentrations were used in T2 treatment seeds.
Salt stress extremely significantly decreased the germination rate and germination energy. Moreover, the effect of salt stress in T2 was considerably greater than that in T1; especially, germination indexs of salt concentrations ≥0.30% were significantly different between T1 and T2. The effects of salt stress on seedling indexes are mainly reflected in seedling height, followed by root length, relative water content, fresh weight, and dry weight. Furthermore, malondialdehyde content, superoxide dismutase activity, and proline content increased significantly with increasing salt concentration, whereas soluble sugar content was the highest at a salt concentration of 0.40%. K+ content in the roots significantly decreased with increasing salt concentration, but the response of Na+ content was not significant (P<0.05).
Salt stress inhibited the germination of C. florida seeds, and pre?treatment with salt enhanced the inhibition. Moreover, the growth indicators, biochemical indicators, and ion absorption of seedlings significantly differed with increasing salt concentration. Comprehensively, seed germination and seedling growth of C. florida were normal under a salt concentration of 0.30%. Thus, C. florida is a candidate species for ornamental use in salt?rich coastal areas, but further field cultivation is needed before extensive application.
大花四照花 / 盐胁迫 / 种子 / 发芽指标 / 生化指标
Cornus florida / salt stress / seed / germination index / biochemical parameters
1 | 李建国,濮励杰,朱明,等. 土壤盐渍化研究现状及未来研究热 点[J].地理学报,2012,67(9): 1233-1245. |
1 | Ll J G,PU L J, ZHU M,et al.The present situation and hot issues in the salt?affected soil research [J]. Acta Geogr Sin, 2012, 67(9): 1233-1245. |
2 | 葛云, 程知言, 胡建, 等. 不同秸秆利用方式下江苏滨海盐碱地盐碱障碍调控[J]. 江苏农业科学,2018, 46(2): 223-227. |
2 | GE Y, CHENG Z Y, HU J,et al. Regulation of saline? alkali barrier in coastal saline?alkali land in Jiangsu Province under different straw utilization patterns [J]. Jiangsu Agric Sci, 2018, 46(2): 223-227. DOl:10.15889/j.issn.1002-1302. 2018.02.056. |
3 | 张 恒,刘晓婷,陈 嵩,等. 盐胁迫下三倍体小黑杨杂种无性系叶片蛋白质差异表达分析[J]. 南京林业大学学报(自然科学版),2020,44(2):59-66. |
3 | ZHANG H,LIU X T,CHEN S,et al.Analysis of differentially expressed proteins in leaves of triploid Populus simonii × P. nigra hybrid clones under salt stress[J].Journal of Nanjing Forestry University(Natural Science Edition),2020,44(2):59-66. DOI:10.3969/j.issn.1000-2006. 201904027. |
4 | 刘凤歧,刘杰淋,朱瑞芬,等.4种燕麦对NaCl胁迫的生理响 应及耐盐性评价[J].草业学报,2015,24(1): 183-189. |
4 | LIU F Q,LIU J L,ZHU R F,et al.Physiological responses and tolerance of four oat varieties to salt stress[J]. Acta Prataculturae Sin, 2015,24(1): 183-189. DOl:10.11686/cyxb20150122. |
5 | CHENG Y E,DONG M Y,FAN X W, et al. A study on cassava tolerance to and growth responses under salt stress[J]. Environ Exp Bot,2018,155: 429-440. DOl:10.1016/j.envexpbot.2018. 07.022. |
6 | HATTA H.Branching principles governing the architecture of Cornus kousa (Comaceae)[J]. Ann Bot, 1999,84(2): 183-193. DOl:10.1006/anbo.1999.0906. |
7 | HADZlABDlC D,ElTZPATRlCK B M, WANG X, et al.Analysis of genetic diversity in flowering dogwood natural stands using microsa? tellites:the effects of dogwood anthracnose[J]. Genetica, 2010, 138(9/10): 1047-1057. DOl:10.1007/s10709-010-9490-8. |
8 | 马青江,孙操稳,张乐英,等.东亚四照花群体中国潜在适生区预测研究[J].南京林业大学学报(自然科学版),2019,43(5):135-140. |
8 | MA Q J,SUN C W,ZHANG L Y,et al. Identification of potential distribution region for East Asian dogwoods(Cornus)in China[J]. Journal of Nanjing Forestry University(Natural Science Edition),2019,43(5):135-140. DOI:10.3969/j.issn.1000-2006.201901046. |
9 | 兰红星,华正媛,杨华,等.美国引进大花四照花幼苗生长规律 的初探[J]. 浙江林业科技, 2013,33(2): 63-66. |
9 | LAN H X, HUA Z Y, YANG H, et al. Observation on growth of Cornus florida seedlings[J]. J Zhejiang For Sci Technol, 2013,33(2): 63-66. DOl:10.3969/j.issn.1001-3776.2013.02.015. |
10 | FU X X, LIU H N, ZHOU X D, et al. Seed dormancy mechanism and dormancy breaking techniques for Cornus kousa var. chinensis[J].Seed Sci Technol, 2013, 41(3): 458-463. DOl: 10. 15258/sst.2013.41.3.12. |
11 | 宋松泉, 程红焱, 姜孝成, 等. 种子生物学[M]. 北京: 科学 出版社, 2008. |
11 | SONG S Q, CHENG H Y, JlANG X C, et al. Seed biology[M]. Beijing: Science Press, 2008. |
12 | 王学奎, 黄见良. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社,2015. |
12 | WANG X K, HLANG J L. Experimental principles and techniques of plant physiology and biochemistry[M]. Beijing: Higher Education Press, 2015. |
13 | 胡生荣,高永,武飞,等.盐胁迫对两种无芒雀麦种子萌发的影响[J]. 植物生态学报, 2007,31(3): 513-520. |
13 | HU S R,GAO Y, WU F, et al. Effect of salt stress on germination of Bromus stamineus and Bromus inermis cv. Xilguole[J]. J Plant Ecol Chin Version, 2007,31(3): 513-520. |
14 | 贾永正,张子晗,喻方圆,等.盐胁迫对紫薇种子萌发特性的影响[J]. 种子,2016, 35(10): 87-91, 94. |
14 | JlA Y Z,ZHANG Z H, YU F Y,et al. Effects of salt stress on germination characteristics of Lagerstroemia indica seeds[J]. Seed, 2016,35(10):87-91, 94. DOl:10.16590/j.cnki.1001-4705.2016.10.087. |
15 | 郁万文, 曹福亮, 蔡金峰, 等.盐碱胁迫对喜树幼苗生长及体内离子选择性运输的影响1)[J].东北林业大学学报,2015,43(11): 1-5. |
15 | YU W W,CAO F L,CAI J F,et al.Effects of saline and alkali stresses on growth and mineral nutrition selective transportation of Camptotheca acuminata Decne[J]. J Northeast For Univ, 2015,43(11): 1-5. DOl:10.3969/j.issn.1000-5382.2015.11.001. |
16 | 王树凤,胡韵雪,孙海菁,等.盐胁迫对2种栎树苗期生长和根 系生长发育的影响[J]. 生态学报, 2014,34(4): 1021-1029. |
16 | WANG S F, HU Y X, SUN H J, et al. Effects of salt stress on growth and root development of two oak seedlings[J]. Acta Ecol Sin, 2014,34(4): 1021-1029.DOl:10.5846/stxb201209291363. |
17 | 刘爱荣,张远兵,陈登科.盐胁迫对盐芥(Thellungiella halophila)生长和抗氧化酶活性的影响[J]. 植物研究,2006, 26(2):2216-2221. |
17 | LIU A R,ZHANG Y B,CHEN D K.Effects of salt stress on the growth and the antioxidant enzyme activity of Thellungiella halophila [J]. Bull Bot Res, 2006, 26(2): 2216-2221. |
18 | 周旋,申璐,肖霄,等.外源水杨酸对盐胁迫下茶树生长及抗氧 化酶活性的影响[J]. 西北农业学报,2014,23(6): 127- 133. |
18 | ZHOU X,SHEN L, XlAO X, et al. Effects of exogenous salicylic acid on growth and antioxidant enzyme activities of tea plant (Camellia sinensis) under salt stress[J] .Acta Agric Boreali-Occiden-talis Sin, 2014,23(6): 127-133. DOl:10.7606/j.issn.1004-1389.2014.06.019. |
19 | 刘文瑜,杨宏伟,魏小红,等.外源NO调控盐胁迫下蒺藜苜蓿 种子萌发生理特性及抗氧化酶的研究[J]. 草业学报, 2015,24(2): 85-95. |
19 | LIU W Y,YANG H W,WEI X H, et al. Effectsof ex?ogenous nitric oixde on seed germination, physiological characteristics and active oxygen metabolism of Medicago truncatula under NaCl stress[J]. Acta Prataculturae Sin, 2015, 24(2): 85-95. DOl:10.11686/cyxb20150211. |
20 | 石元豹,汪贵斌,黄广远,等.3个臭椿半同胞家系耐盐性比较 研究[J]. 四川农业大学学报, 2016,34(2): 153-160. |
20 | SHI Y B, WANG G B, HUANG G Y, et al. Salt tolerance of 3 half?sib families of Ailanthus altissima[J]. J Sichuan Agric Univ, 2016,34?(2)?: 153-160. DOl:10.16036/j.issn.1000-2650. 2016. 02.005. |
21 | CRAMER G R, L?UCHLl A, EPSTElN E. Effects of NaCl and CaCl2 on lon activities in complex nutrient solutions and root growth of cotton[J]. Plant Physiol, 1986,81(3): 792-797. DOl: 10.1104/pp.81.3.792. |
22 | PARlDA A K, DAS A B. Salt tolerance and salinity effects on plants:a review[J]. Ecotoxicol Environ Saf, 2005, 60(3): 324-349. DOl:10.1016/j.ecoenv.2004.06.010. |
23 | 张露婷, 吴江, 梅丽, 等.喜树种源耐盐能力评价及耐盐指标筛选[J]. 林业科学, 2011,47(11): 66-72. |
23 | ZHANG L T,WU J, MEI L, et al. Saline tolerance of Camptotheca acuminata provenances and the index selection for saline tolerance[J] .Sci Silvae Sin, 2011,47(11): 66-72. |
24 | 林选栋, 武文莉, 林丽果, 等.不同盐胁迫水平下硅对高羊茅幼苗生物量、酶活性和渗透调节物质的影响[J]. 草业科学, 2018,35(7): 1653-1660. |
24 | LlN X D, WU W L, LlN L G,et al. Effect of silicon supplies on biomass and antioxidase and osmolytes of tall fescue seedlings under different salt concentration conditions[J].Pratacultural Sci, 2018,35(7): 1653-1660.DOl:10.11829/j.issn.1001-0629.2017-0382. |
/
〈 |
|
〉 |