南京林业大学学报(自然科学版) ›› 2020, Vol. 44 ›› Issue (1): 63-71.doi: 10.3969/j.issn.1000-2006.201903057
魏黔春1,2(), 江泽平2,3, 刘建锋1,2, 史胜青1,2, 赵秀莲1,2, 常二梅1,2,*()
收稿日期:
2019-03-21
修回日期:
2019-09-03
出版日期:
2020-02-08
发布日期:
2020-02-02
通讯作者:
常二梅
作者简介:
魏黔春( 基金资助:
WEI Qianchun1,2(), JIANG Zeping2,3, LIU Jianfeng1,2, SHI Shengqing1,2, ZHAO Xiulian1,2, CHANG Ermei1,2,*()
Received:
2019-03-21
Revised:
2019-09-03
Online:
2020-02-08
Published:
2020-02-02
Contact:
CHANG Ermei
摘要:
【目的】 提高侧柏古树的嫩枝扦插生根率,建立侧柏古树扦插繁殖技术体系。【方法】 采用3种植物生长调节剂[ABT1,NAA和IBA+NAA(等质量比)]的3组质量浓度(200~600,1 000~3 000和4 000~10 000 mg/L)处理采自5、100、300、700 a侧柏的插穗,对插穗生根进程、生根性状(生根率、生根数量、最长根长)、生根进程中营养物质(可溶性淀粉、可溶性糖)含量进行动态测定。【结果】 通过观察发现:0~35 d为愈伤组织形成期;35~65 d为不定根形成期;65~95 d为不定根伸长期。50 a侧柏以南面上部的插穗生根效果较好,生根率为40.8%。100 a侧柏6月扦插的生根率为20.1%。5 a用质量浓度600 mg/L的ABT1处理4 h时的生根效果最好,而100、300、700 a侧柏插穗用中浓度的IBA+NAA (1:1)处理时扦插生根率较高。进一步筛选发现质量浓度为1 000 mg/L的IBA+NAA (等质量比)处理1 min的插穗生根效果最好。全部年龄段侧柏插穗在愈伤组织形成期,可溶性糖、淀粉含量都上升,而5 a侧柏插穗上升的幅度最大。【结论】 选择侧柏古树幼嫩枝条夏季扦插有利于促进插穗不定根的形成。
中图分类号:
魏黔春,江泽平,刘建锋,等. 侧柏古树扦插试验及插穗营养物质变化[J]. 南京林业大学学报(自然科学版), 2020, 44(1): 63-71.
WEI Qianchun, JIANG Zeping, LIU Jianfeng, SHI Shengqing, ZHAO Xiulian, CHANG Ermei. Effects of several factors on rooting of cutting propagation of ancient Platycladus orientalias and the changes of nutritive material[J].Journal of Nanjing Forestry University (Natural Science Edition), 2020, 44(1): 63-71.DOI: 10.3969/j.issn.1000-2006.201903057.
表1
生长调节剂、处理浓度、处理时间编号"
类别 type | 处理/ (mg·L-1) treatment concentration | 浓度 编号 No. | 生长调节剂 growth regulator | 调节剂 编号 No. | 处理时间 treatment time | 时间 编号 No. |
---|---|---|---|---|---|---|
低浓度 low soncen- tration | 200 | B1 | ABT1 | A1 | 1 h | C1 |
400 | B2 | NAA | A2 | 2 h | C2 | |
600 | B3 | IBA+ NAA (1∶1) | A3 | 4 h | C3 | |
中浓度 middle soncen- tration | 1 000 | D1 | ABT1 | A1 | 30 s | E1 |
2 000 | D2 | NAA | A2 | 1 min | E2 | |
3 000 | D3 | IBA+ NAA (1∶1) | A3 | 3 min | E3 | |
高浓度 high soncen- tration | 4 000 | F1 | ABT1 | A1 | 5 s | G1 |
8 000 | F2 | NAA | A2 | 30 s | G2 | |
10 000 | F3 | IBA+ NAA (1∶1) | A3 | 60 s | G3 |
表3
采集部位对扦插生根的影响"
采条部位 position | 生根率/% rooting rate | 生根数/条 rooting number | 最长根长/m the longest root length |
---|---|---|---|
东上up of east | 36.9 b | 11.0 b | 14.0 b |
东下below of east | 19.0 d | 8.0 cd | 13.3 bc |
南上up of south | 40.8 a | 12.3 a | 15.8 a |
南下below of south | 18.1 e | 7.4 d | 12.0 cde |
西上up of west | 24.6 c | 8.9 c | 12.4 bcd |
西下below of west | 16.1 e | 6.7 d | 12.9 bc |
北上up of north | 7.7 f | 3.4 e | 10.4 e |
北下below of north | 20.1 d | 8.0 dc | 10.9 de |
表4
低浓度生长调节剂处理对不同树龄侧柏插穗生根的影响"
处理 treatment | 5 a | 100 a | 300 a | 700 a | ||||
---|---|---|---|---|---|---|---|---|
生根率/% rooting rate | 生根数/条 rooting number | 生根率/% rooting rate | 生根数/条 rooting number | 生根率/% rooting rate | 生根数/条 rooting number | 生根率/% rooting rate | 生根数/条 rooting number | |
A1B1C2 | 73.9 b | 20.2 c | 19.0 a | 5.0 b | 16.1 b | 2.2 c | 0.0 d | 0.0 e |
A1B2C3 | 38.4 f | 12.7 e | 0.0 c | 0.00 f | 5.5 e | 0.8 d | 3.9 c | 0.7 d |
A1B3C1 | 90.2 a | 23.2 b | 0.0 c | 0.0 f | 20.1 a | 2.8 b | 7.7 bc | 1.0 cd |
A2B1C1 | 66.5 c | 17.3 d | 13.2 b | 3.3 bc | 0.0 f | 0.0 e | 0.0 d | 0.0 e |
A2B2C2 | 32.8 g | 11.8 e | 0.0 c | 0.0 f | 9.3 d | 0.7 d | 20.1 a | 2.3 b |
A2B3C3 | 28.4 g | 9.5 f | 0.0 c | 0.0 f | 0.0 f | 0.0 e | 0.0 d | 0.0 e |
A3B1C3 | 46.9 e | 10.8 f | 0.0 c | 0.0 f | 22.2 a | 4.1 a | 23.1 a | 3.3 a |
A3B2C1 | 89.1 e | 27.6 a | 14.5 b | 2.7 c | 14.8 bc | 2.2 c | 16.1 b | 1.7 c |
A3B3C2 | 67.8 c | 15.2 d | 14.8 b | 4.3 b | 0.0 e | 0.0 e | 0.0 d | 0.0 e |
CK | 56.5 d | 16.6 d | 18.5 a | 7.0 a | 11.0 a | 4.2 a | 3.9 c | 3.2 a |
表5
中浓度生长调节剂处理对不同树龄侧柏插穗生根的影响"
处理 treatment | 5 a | 100 a | 300 a | 700 a | ||||
---|---|---|---|---|---|---|---|---|
生根率/% rooting rate | 生根数/条 rooting number | 生根率/% rooting rate | 生根数/条 rooting number | 生根率/% rooting rate | 生根数/条 rooting number | 生根率/% rooting rate | 生根数/条 rooting number | |
A1D1E2 | 69.9 a | 21.18 b | 20.1 bc | 5.83 b | 17.2 bc | 3.33 d | 25.9 a | 6.83 a |
A2D1E3 | 54.8 b | 20.06 b | 22.5 bc | 5.67 b | 18.5 bc | 5.17 bc | 14.8 b | 2.00 cd |
A3D1E1 | 71.9 a | 24.30 a | 17.7 cd | 5.50 b | 29.3 a | 7.17 a | 18.5 c | 3.83 b |
A1D2E1 | 26.5 e | 16.22 bc | 20.1 bc | 7.17 ab | 16.5 c | 3.00 d | 12.2 d | 2.00cd |
A2D2E2 | 45.4 c | 13.56 d | 19.0 cd | 5.17 b | 0.0 d | 0.00 e | 0.0 e | 0.00 e |
A3D2E3 | 47.7 c | 11.77 de | 26.5 a | 8.83 a | 20.1 b | 3.83 cd | 13.2 d | 2.33 cd |
A1D3E3 | 48.5 c | 15.94 bc | 7.7 d | 3.00 c | 16.1 c | 4.50 c | 16.1 bc | 2.50 c |
A2D3E1 | 20.1 f | 9.36 e | 21.4 bc | 7.33 ab | 17.7 bc | 4.50 c | 17.7 bc | 3.17 b |
A3D3E2 | 34.4 d | 17.24 bc | 25.4 a | 7.17 ab | 19.5 b | 6.00 b | 20.1 b | 2.67 c |
表6
高浓度生长调节剂处理对不同树龄侧柏插穗生根的影响"
处理 treatment | 5 a | 100 a | 300 a | 700 a | ||||
---|---|---|---|---|---|---|---|---|
生根率/% rooting rate | 生根数/条 rooting number | 生根率/% rooting rate | 生根数/条 rooting number | 生根率/% rooting rate | 生根数/条 rooting number | 生根率% rooting rate | 生根数/条 rooting number | |
A1F1G2 | 30.1 e | 5.73 e | 17.7 ab | 5.50 b | 14.3 b | 2.17 bc | 5.5 c | 1.00 cd |
A2F1G3 | 53.9 b | 9.15 b | 19.0 b | 4.50 b | 11.7 c | 2.00 bc | 3.8 d | 1.00 cd |
A3F1G1 | 53.1 b | 8.50 bc | 20.1 b | 6.17 ab | 20.1 a | 3.83 a | 16.1 a | 3.50 a |
A1F2G1 | 67.8 a | 11.77 a | 25.6 a | 7.83 a | 12.2 c | 1.50 d | 10.6 b | 1.67 bc |
A2F2G3 | 26.5 e | 6.22 d | 3.8 d | 1.00 d | 17.7 ab | 2.67 b | 9.3 bc | 2.33 b |
A3F2G2 | 41.5 d | 7.85 cd | 9.3 c | 2.50 c | 16.1 ab | 2.83 b | 11.5 b | 1.33 bc |
A1F3G3 | 53.8 b | 9.12 b | 20.3 b | 4.83 b | 13.2 b | 1.67 d | 3.8 d | 0.67 d |
A2F3G1 | 48.5 c | 8.15 bc | 20.1 b | 5.17 b | 14.8 b | 2.00 bc | 0.0 e | 0.00 e |
A3F3G2 | 61.7 ab | 9.41 b | 0.0 e | 0.00 e | 0.0 d | 0.00 e | 0.0 e | 0.00 e |
表7
不同浓度与时间的A3处理对不同树龄侧柏插穗生根的影响"
处理 treatment | 5 a | 100 a | 300 a | 700 a | ||||
---|---|---|---|---|---|---|---|---|
生根率/% rooting rate | 生根数/条 rooting number | 生根率/% rooting rate | 生根数/条 rooting number | 生根率/% rooting rate | 生根数/条 rooting number | 生根率/% rooting rate | 生根数/条 rooting number | |
A3B1C3 | 26.5 e | 9.18 e | 20.1 c | 5.32 c | 17.7 d | 4.07 e | 20.1 a | 4.89 cd |
A3B2C2 | 31.9 d | 10.85 e | 19.0 cd | 4.37 cd | 0.0 f | 0.00 f | 0.0 d | 0.00 g |
A3B2C1 | 44.6 cd | 17.96 cd | 10.6 e | 3.64 e | 21.4 c | 5.40 d | 20.1 a | 6.52 a |
A3D1E3 | 40.8 cd | 16.76 cd | 26.6 b | 8.53 b | 0.0 f | 0.00 f | 19.0 a | 5.24 b |
A3D1E2 | 63.5 a | 26.25 a | 29.3 a | 11.32 a | 23.5 ab | 6.80 ab | 17.7 b | 4.22 d |
A3D1E2 | 53.1 b | 22.10 b | 20.8 c | 5.09 cd | 26.5 a | 7.11 a | 17.7 b | 3.20 e |
A3D2E1 | 57.3 b | 24.54 a | 21.4 c | 5.60 c | 24.6 b | 6.41 ab | 16.1 b | 2.80 ef |
A3D3E1 | 33.6 d | 9.98 e | 11.0 de | 4.31 cd | 9.3 e | 4.63 e | 3.8 c | 1.55 f |
A3F1E1 | 49.2 bc | 19.90 bc | 12.2 e | 5.45 c | 11.5 e | 3.11 f | 0.0 d | 0.00 g |
表8
不同试验因素对侧柏扦插生根效果的方差分析结果"
处理因素 treatment factors | 生根率/% rooting rate | 生根数/条 rooting number | 最长根长/m the longest root length | |||
---|---|---|---|---|---|---|
F | P | F | P | F | P | |
枝条部位cutting position | 5.894 | 0.002** | 3.272 | 0.023* | 0.677 | 0.690 |
扦插时间cutting time | 17.383 | 0.000*** | 9.407 | 0.002** | 42.055 | 0.000*** |
树龄tree age | 38.686 | 0.000*** | 46.164 | 0.000*** | ||
生长调节剂处理growth regulator treatment | 5.906 | 0.038* | 4.436 | 0.066 | - | - |
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