碳源对不同产地香樟体细胞胚胎发生及植株再生的影响

荆茹月; 王佩兰; 黄振; 何林骏; 李志辉

doi:10.3969/j.issn.1000-2006.201904002

荆茹月, 王佩兰, 黄振, 何林骏, 李志辉

南京林业大学学报（自然科学版） ›› 2020, Vol. 44 ›› Issue (1) : 54-62.

PDF(2355 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(2355 KB)

南京林业大学学报（自然科学版） ›› 2020, Vol. 44 ›› Issue (1) : 54-62. DOI: 10.3969/j.issn.1000-2006.201904002

研究论文

碳源对不同产地香樟体细胞胚胎发生及植株再生的影响

荆茹月 ¹ ,
王佩兰 ¹ ,
黄振 ² ,
何林骏 ¹ ,
李志辉 ¹^,^*

作者信息 +

Effects of different carbon sources on somatic embryo of Cinnamomum camphora L.

JING Ruyue ¹ ,
WANG Peilan ¹ ,
HUANG Zhen ² ,
HE Linjun ¹ ,
LI Zhihui ¹^,^*

Author information +

文章历史 +

摘要

【目的】 探究渗透调节物质种类及其不同浓度对香樟体细胞胚胎发生及植株再生的影响,为提高香樟体胚诱导率、优化体细胞胚胎发生体系奠定理论基础。【方法】 以7月初在湖南省郴州、长沙、娄底及怀化市采集的香樟(Cinnamomum camphora L.)的未成熟合子胚为试验材料,分析不同碳源种类、浓度及组合方式对香樟体胚诱导、芽萌发、生根等方面的影响。【结果】 适合香樟的体胚诱导培养基为MS+1.0 mg/L的6-苄氨基腺嘌呤(6-BA)+0.3 mg/L的2,4-二氯苯氧乙酸(2,4-D)+1.0 mg/L 链霉素+15 g/L 蔗糖+30 g/L 山梨醇+6.5 g/L 琼脂+700 mg/L水解酪蛋白;增殖培养基为MS+15 g/L 蔗糖+30 g/L 山梨醇+6.5 g/L 琼脂+700 mg/L 水解酪蛋白;芽萌发培养基为MS+0.1 mg/L 苯基噻二唑基脲(TDZ)+0.5 mg/L 萘乙酸(NAA)+0.1 mg/L 吲哚丁酸(IBA)+15 g/L 蔗糖+15 g/L 山梨醇+6.5 g/L 琼脂;生根培养基为MS+1.0 mg/L IBA+15 g/L 蔗糖+15 g/L 山梨醇+6.5 g/L 琼脂。【结论】 经过改良,添加15 g/L蔗糖和30 g/L山梨醇的组合碳源,香樟体胚的诱导率明显提高;添加15 g/L蔗糖和15 g/L山梨醇的组合碳源处理的新植株生根条数多、主根明显,生长状况更优。

Abstract

【Objective】 In this study, we investigated the effects of somotic adjustment substances on somatic embryogenesis and regeneration of Cinnamomum camphora L. plants, which will provide a foundation for enhancing the induction rate of somatic embryogenesis and optimizing a somatic embryogenesis system for this species. 【Method】 The immature zygotic embryos of C. camphora collected in early July in Chenzhou, Changsha, Loudi and Huaihua City were used as materials for examining the effects of different carbon source types, concentrations, and combinations on somatic embryogenesis. The somatic embryo induction rate, germination rate, and rooting rate of plants were determined under different conditions.【Result】 The suitable medium for somatic embryo induction was MS medium+1.0 mg/L 6-benzylaminopurine (6+BA)+0.3 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D)+1.0 mg/L streptomycin+15 g/L sucrose+30 g/L sorbitol+6.5 g/L agar+700 mg/L hydrolyzed casein; a suitable proliferation medium was MS+15 g/L sourse +30 g/L sorbitol+6.5 g/L agar+700 mg/L hydrolyzed casein; a suitable medium for bud germination was murashige and skoog medium+0.1 mg/L thidiazuron (TDZ)+0.5 mg/L naphthylacetic acid (NAA)+0.1 mg/L 3-indolebutyric acid (IBA)+15 g/L sucrose+15 g/L sorbitol+6.5 g/L agar; and a suitable medium for rooting was MS+1.0 mg/L IBA+15 g/L sucrose+15 g/L sorbitol+6.5 g/L agar. 【Conclusion】 As a consequence of modification, somatic embryo induction was significantly improved by adding 15 g/L sucrose and 30 g/L sorbitol as combined carbon sources, and the new plants thus generated had a larger number of rooting strips, obvious main roots, and better growth characteristics.

导出引用

荆茹月, 王佩兰, 黄振, 等. 碳源对不同产地香樟体细胞胚胎发生及植株再生的影响[J]. 南京林业大学学报（自然科学版）. 2020, 44(1): 54-62 https://doi.org/10.3969/j.issn.1000-2006.201904002

JING Ruyue, WANG Peilan, HUANG Zhen, et al. Effects of different carbon sources on somatic embryo of Cinnamomum camphora L.[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2020, 44(1): 54-62 https://doi.org/10.3969/j.issn.1000-2006.201904002

中图分类号： S792.23

参考文献

列表( 原文顺序 | 文献年度倒序 | 文中引用次数倒序 ) 可视化分析

[1]

邹晖, 陈永快, 王伟英. 香樟组培快繁技术研究进展[J]. 福建农业科技, 2009(1):71-72.

ZOU

, CHEN

Y K

, WANG

W Y

. Research progress on tissue culture and rapid propagation technology of Cinnamomum camphora L. [J]. Fujian Agricultural Science and Technology, 2009(1):71-72. DOI: 10.13651/j.cnki.fjnykj.2009.01.043.

https://doi.org/10.13651/j.cnki.fjnykj.2009.01.043

本文引用 [1]

[2]

毛春英. 香樟的引种与驯化研究[J]. 山东农业大学学报(自然科学版), 2004, 35(4):534-539.

MAO

C Y

. Research on introduction and domestication of Cinnamomum camphora (Linn) Presl [J]. Journal of Shandong Agricultural University(Natural Science), 2004, 35(4):534-539. DOI: 10.3969/j.issn.1000-2324.2004.04.014.

https://doi.org/10.3969/j.issn.1000-2324.2004.04.014

本文引用 [1]

[3]

叶润燕, 童再康, 张俊红, 等. 樟树茎段组培快繁[J]. 浙江农林大学学报, 2016, 33(1):177-182.

R Y

, TONG

Z K

, ZHANG

J H

, et al. Tissue culture and rapid propagation for stems of Cinnamomum camphora [J]. Journal of Zhejiang A&F University, 2016, 33(1):177-182. DOI: 10.11833/j.issn.2095-0756.2016.01.024.

https://doi.org/10.11833/j.issn.2095-0756.2016.01.024

本文引用 [1]

[4]

戴小英, 章挺, 汪信东, 等. 樟树组织培养技术研究进展[J]. 江西农业大学学报, 2017, 39(5):900-906.

DAI

X Y

, ZHANG

, WANG

X D

, et al. The advance in the research on the tissue culture of Cinnamomum camphora [J]. Acta Agriculturae Universitatis Jiangxiensis(Natural Sciences Edition), 2017, 39(5):900-906. DOI: 10.13836/j.jjau.2017116.

https://doi.org/10.13836/j.jjau.2017116

本文引用 [1]

[5]	辛全伟. 香樟优良无性系繁殖技术的研究[D]. 福州: 福建农林大学, 2010. XIN Q W. Study on propagation techniques of superior clones of Cinnamomum camphora [D]. Fuzhou: Fujian Agriculture and Forestry University, 2010. 本文引用 [1]

[6]

杜丽, 叶要妹, 包满珠. 香樟未成熟合子胚体胚发生及植株再生研究[J]. 林业科学, 2006, 42(6):37-39.

, YE

Y M

, BAO

M Z

. Study on somatic embryogenesis and plant regeneration of immature zygotic embryo of Cinnamomum camphora [J]. Scientia Silvae Sinicae, 2006, 42(6):37-39. DOI: 10.3321/j.issn:1001-7488.2006.06.007.

https://doi.org/10.3321/j.issn:1001-7488.2006.06.007

本文引用 [1]

[7]	蔡玲, 覃子海, 黄金使, 等. 樟脑型樟树芽器官离体培养技术[J]. 广西林业科学, 2013, 42(4):315-318. CAI L, QIN Z H, HUANG J S, et al. Culture technology of buds in Cinnamomum camphora with camphor in vitro [J]. Guangxi Forestry Science, 2013, 42(4):315-318. 本文引用 [1]

[8]

杜丽. 香樟子叶胚体胚发生诱导的研究[J]. 河南农业科学, 2014, 43(10):108-111.

. Research on somatic embryogenesis induction from cotyledonary embryo of Cinnamomum camphora L [J]. Journal of Henan Agricultural Sciences, 2014, 43(10):108-111. DOI: 10.3969/j.issn.1004-3268.2014.10.025.

https://doi.org/10.3969/j.issn.1004-3268.2014.10.025

本文引用 [4]

[9]

杨柳, 李志辉, 林立彬. 激素对不同产地樟树体胚发生的影响[J]. 中南林业科技大学学报, 2018, 38(12):115-120, 135.

YANG

, LI

Z H

, LIN

L B

. Effects of hormon on somatic embryogenesis from different provenances of Cinnamomum camphora [J]. Journal of Central South University of Forestry & Technology, 2018, 38(12):115-120,135. DOI: 10.14067/j.cnki.1673-923x.2018.12.016.

https://doi.org/10.14067/j.cnki.1673-923x.2018.12.016

本文引用 [3]

[10]	KOMAI F, OKUSE I, SAGA K, et al. Improvement on the efficiency of somatic embryogenesis from spinach root tissues by applying various sugars[J]. Journal of the Japanese Society for Horticultural Science, 1996, 65(1):67-72. 本文引用 [1]

[11]	HAZARIKA B N. Acclimatization of tissue cultured plants[J]. Curr Sci, 2003, 85:1704-1711. 本文引用 [1]

[12]	JAVED F, IKRAM S. Effect of sucrose induced osmotic stress on callus growth and biochemical aspects of two wheat genotypes[J]. Pakistan Journal of Botany, 2008, 40(4):1487-1495. 本文引用 [1]

[13]

陈婷婷, 吴小芹, 叶建仁, 等. 抗松材线虫病马尾松体胚发生与植株再生条件的优化[J]. 南京林业大学学报(自然科学版), 2019, 43(3):1-8.

CHEN

T T

, WU

X Q

, YE

J R

, et al. Optimization of somatic embryogenesis and plant regeneration conditions of Pinus massoniana with resistance to disease [J]. Journal of Nanjing Forestry University (Natural Sciences Edition): 2019, 43(3):1-8. DOI: 10.3969/j.issn.1000-2006.201806005.

https://doi.org/10.3969/j.issn.1000-2006.201806005

本文引用 [1]

[14]	施雪萍. 樟树体细胞胚再生体系的优化和转化Barnase、PaFT基因的研究[D]. 武汉: 华中农业大学, 2009. SHI X P. Enhancement of somatic embryogenesis and genetic transformation with Barnase and PaFT genes in Cinnamomum camphora L [D]. Wuhan: Huazhong Agricultural University, 2009. 本文引用 [1]

[15]	ZHANG C X, YAO Z Y, ZHAO Z, et al. Histological observation of somatic embryogenesis from cultured embryos ofQuercus variabilis BI.[J]. Journal of Plant Physiology and Molecular Biology, 2007, 33(1):33-38. 本文引用 [1]

[16]

陈金慧, 施季森, 诸葛强, 等. 杂交鹅掌楸体细胞胚胎发生研究[J]. 林业科学, 2003, 39(4):49-53.

CHEN

J H

, SHI

J S

, ZHUGE

, et al. Studies on the somatic embryogenesis of Liriodendron hybrids(L.chinense × L.tulipifera) [J]. Scientia Silvae Sinicae, 2003, 39(4):49-53. DOI: 10.3321/j.issn:1001-7488.2003.04.008.

https://doi.org/10.3321/j.issn:1001-7488.2003.04.008

本文引用 [2]

[17]

项伟波, 赵金凯, 吴家胜, 等. 香榧体细胞胚发生、发育的形态与细胞学观察[J]. 园艺学报, 2015, 42(8):1477-1486.

XIANG

W B

, ZHAO

J K

, WU

J S

, et al. Morphological and cytological observation of somatic embryogenesis and development in Torreya grandis ‘Merrillii’ [J]. Acta Horticulturae Sinica, 2015, 42(8):1477-1486. DOI: 10.16420/j.issn.0513-353x.2015-0039.

https://doi.org/10.16420/j.issn.0513-353x.2015-0039

本文引用 [1]

[18]	龙健梅. 柑橘珠心胚起始相关miRNA挖掘与csi-miR156a调控体细胞胚发生作用机理[D]. 武汉: 华中农业大学, 2017. LONG J M. Identification of miRNA involved inCitrus nucellar embryo initiation and regulatory mechanism of csi-miR156a in somatic embryogenesis[D]. Wuhan: Huazhong Agricultural University, 2017. 本文引用 [1]

[19]

王亚馥, 崔凯荣, 陈克明, 等. 小麦组织培养中体细胞胚胎发生的细胞胚胎学及淀粉消长动态的研究[J]. 实验生物学报, 1993, 26(3):259-267.

WANG

Y F

, CUI

K R

, CHEN

K M

, et al. Studies on cell embryology and dynamics of starch during somatic embryogenesis of wheat(Triticum aestivum L.) [J]. Acta Biologiae Experimentalis Sinica, 1993, 26(3):259-267.

本文引用 [1]

[20]

沈海龙, 高翔翔, 杨玲. 甘露醇、蔗糖和低温预处理对花楸体细胞胚诱导的影响[J]. 植物生理学通讯, 2008, 44(4):677-681.

SHEN

H L

, GAO

X X

, YANG

. Effects of mannitol, sucrose and cold pretreatment on somatic embryogenesis of Sorbus pohuashanensis (Hance) Hedl [J]. Plant Physiology Communications, 2008, 44(4):677-681. DOI: 10.13592/j.cnki.ppj.2008.04.047.

https://doi.org/10.13592/j.cnki.ppj.2008.04.047

本文引用 [1]

[21]

张俊娥. 5种碳源对柑橘愈伤组织生长及体细胞胚胎发生能力的影响[J]. 江西师范大学学报(自然科学版), 2010, 34(2):211-214.

ZHANG

J E

. The effects of five carbon sources to growth speed of callus and somatic embryogenesis in Citrus [J]. Journal of Jiangxi Normal University(Natural Sciences Edition), 2010, 34(2):211-214. DOI: 10.3969/j.issn.1000-5862.2010.02.024.

https://doi.org/10.3969/j.issn.1000-5862.2010.02.024

本文引用 [1]

[22]

吴丽芳, 魏晓梅. 渗透剂对白刺花体细胞胚成熟及萌发的影响[J]. 广西植物, 2019, 39(8) : 1107-1114.

L F

, WEI

X M

. Effects of penetrant on maturation and germination for somatic embryo of Sophora davidii [J]. Guangxi Plants, 2019, 39(8) : 1107-1114. DOI: 10.11931/guihaia.gxzw201810021.

https://doi.org/10.11931/guihaia.gxzw201810021

本文引用 [1]

[23]	李立娟. 深入探究“植物细胞吸水、失水原理”[J]. 生物学通报, 2017, 52(6):45-46. LI L J. In-depth study of the principle of water uptake and dehydration of plant cells[J]. Bulletin of Biology, 2017, 52(6):45-46. 本文引用 [1]

[24]

杨玲, 刘虹男, 张冬严, 等. 生长调节剂和渗透调节物质对水曲柳体胚发生的影响[J]. 植物研究, 2017, 37(5):682-689.

YANG

, LIU

H N

, ZHANG

D Y

, et al. Effect of plant growth regulators and osmoticums on somatic embryogenesis of Fraxinus mandshurica Rupr .[J]. Bulletin of Botanical Research, 2017, 37(5):682-689. DOI: 10.7525/j.issn.1673-5102.2017.05.007.

https://doi.org/10.7525/j.issn.1673-5102.2017.05.007

本文引用 [2]

[25]

黄镜浩, 谢江辉, 王松标, 等. 扁桃杧(Mangifera persiciformis)体胚发生及再生体系建立[J]. 果树学报, 2009, 26(2):176-179, 264.

HUANG

J H

, XIE

J H

, WANG

S B

, et al. Studies on somatic embryogenesis and plant regeneration of Mangifera persiciformis [J]. Journal of Fruit Science, 2009, 26(2):176-179, 264. DOI: 10.13925/j.cnki.gsxb.2009.02.014.

https://doi.org/10.13925/j.cnki.gsxb.2009.02.014

本文引用 [1]

[26]

胡恒康, 江香梅, 张启香, 等. 碳源对山核桃体细胞胚发生和植株再生的影响[J]. 浙江农林大学学报, 2011, 28(6):911-917.

H K

, JIANG

X M

, ZHANG

Q X

, et al. Somatic embryogenesis and plant regeneration fromCarya cathayensis embryos using different carbon sources [J]. Journal of Zhejiang Agriculture and Forestry University, 2011, 28(6):911-917. DOI: 10.3969/j.issn.2095-0756.2011.06.012.

https://doi.org/10.3969/j.issn.2095-0756.2011.06.012

本文引用 [1]

[27]

GARIN

, BERNIER-CARDOU

, ISABEL

, et al. Effect of sugars, amino acids, and culture technique on maturation of somatic embryos of Pinus strobus on medium with two gellan gum concentrations[J]. Plant Cell, Tissue and Organ Culture, 2000, 62(1):27-37. DOI: 10.1023/A:1006402215457.

https://doi.org/10.1023/A:1006402215457

http://link.springer.com/10.1023/A:1006402215457

本文引用 [1]

[28]

刘杜玲, 吕平会, 彭少兵, 等. 板栗愈伤组织诱导及外植体褐化研究[J]. 中南林业科技大学学报, 2016, 36(12):26-30.

LIU

D L

, LV

P H

, PENG

S B

, et al. Research on callus induction and explant browning of chestnut (Castanea mollissima Bl.) [J]. Journal of Central South University of Forestry & Technology, 2016, 36(12):26-30. DOI: 10.14067/j.cnki.1673-923x.2016.12.005.

https://doi.org/10.14067/j.cnki.1673-923x.2016.12.005

本文引用 [1]

[29]	陈碧华, 张娟, 范辉华. 沉水樟组织培养技术研究[J]. 种子, 2016, 35(2):121-123. CHEN B H, ZHANG J, FAN H H. Tissue culture technique of Cinnamomum micranthum [J]. Seed, 2016, 35(2):121-123. DOI: 10.16590/j.cnki.1001-4705.2016.02.121. https://doi.org/10.16590/j.cnki.1001-4705.2016.02.121 本文引用 [1]

[30]

彭春雪, 崔雪梅, 刘春苹, 等. 3种抗褐化剂影响水曲柳体胚发生的生理分析[J]. 植物研究, 2019, 39(2):252-258.

PENG

C X

, CUI

X M

, LIU

C P

, et al. Physiological analysis of three anti-browning agents affecting somatic embryogenesis in Fraxinus mandshurica [J]. Bulletin of Botanical Research, 2019, 39(2):252-258. DOI: 10.7525/j.issn.1673-5102.2019.02.011.

https://doi.org/10.7525/j.issn.1673-5102.2019.02.011

本文引用 [1]

[31]	吴婉婉, 孙威江, 陈志丹. 茶树茎段组织培养过程中防褐化污染方法研究[J]. 中国茶叶, 2019, 41(1):22-24. WU W W, SUN W J, CHEN Z D. Study on the method of preventing browning pollution in the tissue culture process of tea stems[J]. China Tea, 2019, 41(1):22-24. 本文引用 [1]

[32]

张鑫. 几种防褐剂对阿月浑子种胚培养褐化现象抑制效应研究[J]. 天津农林科技, 2018(1):7-8, 12.

ZHANG

. Study on the inhibitory effect of several anti-browning agents on browning of Pistacia vera L. seed embryos [J]. Science and Technology of Tianjin Agriculture and Forestry, 2018(1):7-8, 12. DOI: 10.16013/j.cnki.1002-0659.2018.0004.

https://doi.org/10.16013/j.cnki.1002-0659.2018.0004

本文引用 [1]

[33]

吴丽君, 叶建仁. 火炬松体胚发生与几种专利培养基的应用[J]. 南京林业大学学报(自然科学版), 2008, 32(1):1-5.

L J

, YE

J R

. A study on somatic embryogenesis in loblolly pine and application of several patent media[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2008, 32(1):1-5. DOI: 10.3969/j.issn.1000-2006.2008.01.001.

https://doi.org/10.3969/j.issn.1000-2006.2008.01.001

本文引用 [1]