通过对毛竹(Phyllostachys edulis)GeBP转录因子分子特征和表达模式的研究，为揭示其在表皮毛形成过程中的作用提供参考。

通过生物信息学方法对毛竹GeBP转录因子的基因结构、蛋白基本理化性质与结构特征以及系统进化进行系统分析；采用转录组数据，对各成员基因在毛竹不同组织中的表达模式进行分析，同时利用实时定量PCR(qRT?PCR)的方法进行验证。

在毛竹基因组中共鉴定出16个GeBP转录因子家族基因成员(PeGeBP1-PeGeBP16)，其中仅3个基因(PeGeBP3、PeGeBP4和PeGeBP5)含有内含子，数量分别为1、1和5个。PeGeBPs编码的蛋白主要定位在细胞核内，蛋白分子质量为23.37～62.17 ku，理论等电点为5.02~10.14。系统进化分析表明，毛竹与水稻(Oryza sativa)和二穗短柄草(Brachypodium distachyum)对应的GeBP成员亲缘关系较近，分别聚类到4个分支，而与拟南芥(Arabidopsis thaliana)和毛果杨(Populus trichocarpa)的距离较远。转录组表达谱热图分析显示，除PeGeBP5在叶片中未检测到表达外，其他基因在鞭生根、茎生根、笋、叶片和箨片中均有表达，但表达丰度具有一定的差异。qRT?PCR结果表明，16个PeGeBPs在叶、笋、箨、箨片和纤毛中均检测到表达，但存在明显差异，在有表皮毛的组织（叶、箨、箨片和纤毛）中12个PeGeBPs的表达量均高于无表皮毛的笋，只有PeGeBP16在笋中表达量高于其他组织，而其他3个基因在笋中的表达量也较低。

从毛竹中鉴定了16个GeBP转录因子基因，各基因在不同的组织中表达量差异明显，表明它们功能的多样性。大多数PeGeBPs(12个)的表达量在有表皮毛的组织中均高于无表皮毛的组织，表明这些基因可能参与表皮毛的形成调控。

Molecular characteristics and expression pattern of GeBP transcription factors (TFs) in moso bamboo (Phyllostachys edulis) were studied in order to help reveal the role of GeBP TFs involved in the formation of trichomes.

Bioinformatic tools were used for systematic analyses of GeBP TFs in moso bamboo, including the online software GSDS 2.0 to examine gene structure, ExPaSy to investigate basic physicochemical properties, and Plant mPLoc to study protein structural characteristics and their subcellular locations. Phylogenetic analyses were performed using MEGA6.0 and amino acid sequences of GeBPs of different species. Transcriptome data generated from different tissues such as rhizome roots, stem roots, shoots, leaves and sheath blades were used to analyze expression patterns of GeBP genes in moso bamboo. Quantitative reverse?transcription PCR (qRT?PCR) was performed to assess expression patterns of GeBP genes using cDNA of different amounts of trichome tissue.

A total of 16 members of the GeBP gene family were identified in the genome of moso bamboo (PeGeBP1 to PeGeBP16). Only three PeGeBPs (PeGeBP3, PeGeBP4 and PeGeBP5) contained introns (n = 1， n = 1， n = 5, respectively). The molecular weight of proteins encoded by PeGeBPs ranged from 23.37 to 62.17 ku, and the theoretical isoelectric points ranged from 5.02 to 10.14. Predictions of subcellular locations showed that five PeGeBPs are located in both nucleus and cytoplasm, ten PeGeBPs are in nucleus, and one occurs only in the cytoplasm. Phylogenetic analyses suggested four clades of PeGeBPs with five, four, three and four members, respectively; they appeared to be closely related to those of Oryza sativa and Brachypodium distachyum and distantly related to those of Arabidopsis thaliana and Populus trichocarpa. Transcriptome data indicated that all PeGeBPs are expressed in rhizome roots, stem roots, shoots, leaves and sheath blades, at certain differences, apart from PeGeBP5 which was not expressed in leaves. qRT?PCR results showed that 16 PeGeBPs occurred in leaves, shoots, sheaths, sheath blades and cilia, and significant differences between tissue types were observed. Expression levels of 12 PeGeBPs in tissues containing trichomes such as leaves, sheaths, sheath blades and cilia were higher than those in shoots without trichomes. Only PeGeBP16 showed higher expression levels in shoots than that in other tissues. Moreover, expression levels of the other three genes in bamboo shoots were also low.

Sixteen GeBP genes were identified in moso bamboo, and their expression levels differed significantly between tissues, suggesting functional diversity. Expression levels of 12 out of 16 PeGeBPs were higher in tissues containing trichomes than that in tissues without trichomes, suggesting that these genes may be involved in the regulation of trichome formation.