赤霉素对‘大富贵’芍药休眠解除及内源激素和糖类代谢的影响

doi:10.3969/j.issn.1000-2006.201909019

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南京林业大学学报（自然科学版） ›› 2020, Vol. 44 ›› Issue (3): 26-32.doi: 10.3969/j.issn.1000-2006.201909019

所属专题：球根花卉专题

• 专题报道（执行主编李维林） • 上一篇下一篇

赤霉素对‘大富贵’芍药休眠解除及内源激素和糖类代谢的影响

姜楠南¹^,²(), 张启翔¹, 王媛³, 孙音², 房义福²(), 徐金光²()

^1.花卉种质资源创新与分子育种北京市重点实验室，国家花卉工程技术研究中心，北京林业大学园林学院，北京 100083
^2.山东省林业科学研究院，山东济南 250014
^3.山东省林木种苗和花卉站，山东济南 250014

收稿日期:2019-09-09 修回日期:2019-10-29 出版日期:2020-05-30 发布日期:2020-06-11
通讯作者: 房义福,徐金光
作者简介:姜楠南（abcnnjiang@126.com）
基金资助:
山东省林业科技创新项目（鲁财农指[2018]0019）

Effects of GA₃ on dormancy release, endogenous hormones levels and sugar metabolism in Paeonia lactiflora ‘Da Fugui’

JIANG Nannan¹^,²(), ZHANG Qixiang¹, WANG Yuan³, SUN Yin², FANG Yifu²(), XU Jinguang²()

^1.Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture of Beijing Forestry University, Beijing, 100083, China
^2.Shandong Academy of Forestry, Jinan 250014, China
^3.Forest Seedling and Flower Station of Shandong Province, Jinan 250014, China

Received:2019-09-09 Revised:2019-10-29 Online:2020-05-30 Published:2020-06-11
Contact: FANG Yifu,XU Jinguang

摘要/Abstract

摘要： 目的

研究不同质量浓度赤霉素（GA₃，0、100、200、300、1 000 mg/L）替代低温解除芍药品种‘大富贵’休眠的效果，探讨休眠解除前后其内源激素和糖类代谢生理变化，为生产上利用GA₃解除芍药休眠及调控花期提供理论及技术支撑。

方法

以盆栽露地越冬‘大富贵’为对照，调查不同浓度GA₃处理植株生长、开花的差异，采用高效液相色谱仪测定果糖、葡萄糖、蔗糖含量，采用酶联免疫法测定5种糖类代谢相关酶活性，采用液相色谱-质谱联用测定5种内源激素含量。

结果

①100、200、300、1 000 mg/L GA₃处理均可解除‘大富贵’休眠；休眠解除后植株生长发育有差异，4种 GA₃处理茎折萎率分别为0.88%、18.1%、65.7%、100%；前3种处理可正常开花，但较对照株高降低17.9%～23.5%，成花数量减少46.6%～65.9%，花径减小11.8%～16.7%。②‘大富贵’休眠期、芽鳞开绽期主要可溶性糖为果糖、葡萄糖，芽膨大期蔗糖占比升高；休眠期、芽膨大期、芽鳞开绽期蔗糖合成酶、细胞壁转化酶、液泡转化酶活性呈升高趋势，蔗糖磷酸合成酶、α-淀粉酶先略降后上升。③赤霉素、生长素、脱落酸、水杨酸含量整体呈先降后升趋势，茉莉酸含量则先升后降，变化趋势与其他内源激素相反。

结论

4种浓度GA₃处理均可解除‘大富贵’休眠，100 mg/L GA₃处理对植物生长与促成栽培开花情况最好；休眠期、芽鳞开绽期果糖、葡萄糖为芽内主要可溶性糖，芽膨大期根内营养物质以蔗糖形式转运至芽；外施GA₃后芍药5种内源激素水平发生剧烈变化，植株休眠到生长的转变是各种激素协同作用的结果。

关键词: 赤霉素, 解除休眠, 糖类代谢, 内源激素, 芍药

Abstract: Objective

We investigated the effects of gibberellin acid (GA₃) (0, 100, 200, 300 and 1 000 mg/L) on dormancy release to replace low-temperature treatments; furthermore, physiological changes in endogenous hormones and carbohydrate metabolism were examined. The results may provide theoretical and technical support for applied use of GA₃to terminate dormancy and regulate flowering time in Paeonia lactiflora.

Method

We investigated effects of GA₃ at different concentrations on plant growth and flowering using open-field overwintered potted P. lactiflora ‘Da Fugui’ as a control. We measured fructose, glucose and sucrose concentrations using high performance liquid chromatography (HPLC). Activities of enzymes associated with the metabolism of five sugar compounds and content of five endogenous hormones were determined using enzyme-linked immunoassays (ELISA) and liquid chromatography-mass spectrometry(LC-MS), respectively.

Result

The results showed that 100, 200, 300 and 1 000 mg/L GA₃ affected dormancy termination in P. lactiflora ‘Da Fugui’, and substantial differences in plant growth and development were observed in treated seedlings. Stem lodging and wilting rates of seedlings treated with 100, 200, 300 and 1 000 mg/L GA₃ were 0.88%, 18.1%, 65.7% and 100%, respectively. Treatments with 100-300 mg/L GA₃ led to substantially decreased height (variation rate 17.9%-23.5%), number of flowers (variation rate 46.6%-65.9%), and flower diameter (variation rate 11.8%-16.7%); however, the flowering process was normal. During dormancy and during the bud scale bursting period, fructose and glucose were the predominant soluble sugars in ‘Da Fugui’ plants, and the proportion of sucrose increased du?ring bud swelling. During dormancy, bud swelling, and bud scale bursting, sucrose synthase, cell wall invertase, and vacuolar invertase activity increased, whereas sucrose phosphate synthase and alpha-amylase decreased slightly initially and then increased. Gibberellin, auxin, abscisic acid and salicylic acid content initially decreased and then increased, whereas jasmonic acid content showed the opposite pattern.

Conclusion

Treatments with 100-400 mg/L GA₃ affected dormancy release in ‘Da Fugui’ plants, and the strongest effect was observed at 100 mg/L GA₃ which produced the best growth and flowering results. Fructose and glucose are the main soluble sugars during bud dormancy and bud scale burs?ting, and nutrients produced in the roots are transferred to buds in form of sucrose during bud swelling. GA₃ treatments induced considerable changes in the levels of five endogenous hormones, and subsequent synergistic effects elicited the transition from dormancy to seedling growth.

Key words: gibberellin, dormancy release, sugar metabolism, endogenous hormones, Paeonia lactiflora

中图分类号:

S682.1

姜楠南,张启翔,王媛,等. 赤霉素对‘大富贵’芍药休眠解除及内源激素和糖类代谢的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(3): 26-32.

JIANG Nannan, ZHANG Qixiang, WANG Yuan, SUN Yin, FANG Yifu, XU Jinguang. Effects of GA₃ on dormancy release, endogenous hormones levels and sugar metabolism in Paeonia lactiflora ‘Da Fugui’[J].Journal of Nanjing Forestry University (Natural Science Edition), 2020, 44(3): 26-32.DOI: 10.3969/j.issn.1000-2006.201909019.

图/表 6

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图5

参考文献 35

1	姜楠南, 吴晓星, 王翠香, 等. 芍药周年供花技术研究综述[J]. 林业与环境科学, 2017, 33(4):135-138.
	JIANG N N, WU X X, WANG C X, et al. A review on the annual Paeonia lactiflora supply technology[J]. Forestry and Environmental Science,2017,33(4):135-138. DOI:10.3969/j.issn.1006-4427. 2017.04.026.
2	高秀华, 傅向东. 赤霉素信号转导及其调控植物生长发育的研究进展[J]. 生物技术通报, 2018, 34(7):1-13.
	GAO X H, FU X D. Research progress for the gibberellin signaling and action on plant growth and development[J]. Biotechnology Bulletin, 2018, 34(7):1-13. DOI:10.13560/j.cnki.biotech.bull.1985.2018-0447.
3	张文娟. 冷藏和赤霉素（GA3）处理对牡丹促成栽培影响的研究[D]. 北京：北京林业大学, 2005. ZHANG W X. Study on the effects of chilling and GA3 treatments on forcing culture of tree peony[D]. Beijing: Beijing Forestry University, 2005.
4	吕长平, 成明亮, 莫宁捷, 等. 外源GA₃对芍药花芽发育的影响[J]. 作物研究, 2009, 23(2):133-135.
	LÜ C P, CHENG M L, MO N J, et al. Effects of exogenous GA₃ on flower bud development of peony[J]. Crop Research, 2009, 23(2):133-135. DOI:10.16848/j.cnki.issn.1001-5280.2009.02.020.
5	叶真逍. 外源赤霉素处理对西瓜种子萌发过程中活性氧和糖类代谢、乙醛酸循环的影响[D]. 杭州：浙江农林大学, 2015. YE Z X. The effect of gibberellin pretreatment on active oxygen metabolism, sugar metabolism glyoxylate cycle of watermelon seed during germination[D]. Hangzhou: Zhejiang Agriculture & Forestry University, 2015.
6	GAO Z, ZHANG C J, LUO M, et al. Proteomic analysis of pear (Pyrus pyrifolia) ripening process provides new evidence for the sugar/acid metabolism difference between core and mesocarp [J]. Proteomics, 2016, 16(23): 3025-3041. DOI:10.1002/pmic.201600108.
7	SUN T P. The molecular mechanism and evolution of the GA⁃GID1⁃DELLA signaling module in plants[J]. Current Biology, 2011, 21(9): 338-345. DOI:10.1016/j.cub.2011.02.036.
8	DAVIÈRE J M，ACHARD P. A pivotal role of DELLAs in regulating multiple hormone signals[J]. Mol Plant，2016，9(1)：10-20．DOI:10.1016/j.molp.2015.09.011.
9	HAUVERMALE A L，ARIIZUMI T，STEBER C M. Gibberellin signaling：a theme and variations on DELLA repression[J]. Plant Physiol，2012，160(1)：83-92. DOI:10.1104/pp.112. 200956.
10	高健洲, 张逸璇, 李秉玲, 等. 数量化模型估算观赏植物需冷量研究进展[J]. 中国农学通报, 2018, 34(19):76-82.
	GAO J Z, ZHANG Y X, LI B L, et al. Advances in quantitative model for estimating chilling requirement of ornamental plants [J]. Chinese Agricultural Science Bulletin, 2018, 34(19):76-82. DOI:CNKI:SUN:ZNTB.0.2018-19-013.
11	张志良,瞿伟菁.植物生理学实验指导[M]. 北京:高等教育出版社,2003.
	ZHANG Z L，QU W J. The experimental guide for plant physiology[M]. Beijing:Higer Education Press,2003.
12	耿兴敏,裘建宇,王良桂. 球根花卉花粉萌发过程中糖类物质及相关酶活性的变化[J]. 南京林业大学学报(自然科学版), 2013,37(4):34-38.
	GENG X M, QIU J Y, WANG L G. Changes of carbohydrate content and related enzyme activity during pollen germination of two bulb flowers[J]. Journal of Nanjing Forestry University (Natural Sciences Edition), 2013,37(4):34-38. DOI:10.3969/j.issn.1000-2006.2013.04.007
13	GAI S P, ZHANG Y X, LIU C Y, et al. Transcript profiling of Paoenia ostii during artificial chilling induced dormancy release identifies activation of GA pathway and carbohydrate metabolism[J]. PloS One, 2013, 8(2), e55297. DOI:10.1371/journal.pone.0055297.
14	CHENG F Y, ZHONG Y, LONG F, et al. Chinese herbaceous peonies: cultivar selection for forcing culture and effects of chilling and GA₃ on plant development[J]. Israel Journal of Plant Sciences, 2009, 57(4): 357-367. DOI:10.1560/IJPS.57.4.357.
15	YEO S M, RHIE Y H, LEE S Y, et al. Dormancy release and flowering of Paeonia lactiflora ‘Taebaek’ by natural cumulative chilling and GA₃ treatment[J]. Horticulture, Environment, and Biotechnology, 2012, 53(4):263-270. DOI:10.1007/s13580-012-0037-z.
16	李燕, 梁俊, 楚克欢, 等. 萌芽期苹果花芽中糖含量及相关酶活性的变化[J]. 果树学报, 2016, 33(2):172-178.
	LI Y, LIANG J, CHU K H, et al. A study on carbon metabolism in apple flower bud during budbreak[J]. Journal of Fruit Science, 2016, 33(2):172-178. DOI:10.13925/j.cnki.gsxb.20150314.
17	RUAN Y L. Sucrose metabolism: gateway to diverse carbon use and sugar signaling[J]. Annual Review of Plant Biology, 2014, 65(1):33-67. DOI:10.1146/annurev⁃arplant⁃050213-040251.
18	WANG L, RUAN Y L. Regulation of cell division and expansion by sugar and auxin signaling[J]. Frontiers in Plant Science, 2013, 4:163. DOI:10.3389/fpls.2013.00163.
19	盛毅迪. GABA对大麦及水稻糊粉细胞α⁃淀粉酶生成的诱导效应[D]. 南京:南京农业大学, 2015.
	SHENG Y D. Induction of aleurone α⁃amylase production by GABA in seeds of barley and rice[D]. Nanjing: Nanjing Agricultural University, 2015.
20	WEISS D, ORI N. Mechanisms of cross talk between gibberellin and other hormones[J]. Plant Physiology, 2007, 144(3):1240-1246. DOI:10.1104/pp.107.100370.
21	KOSOVÁ K, PRÁŠIL I T, VÍTÁMVÁS P, et al. Complex phytohormone responses during the cold acclimation of two wheat cultivars differing in cold tolerance, winter Samanta and spring Sandra[J]. Journal of Plant Physiology, 2012, 169( 6) : 567－576. DOI:10.1016/j.jplph.2011.12.013.
22	张永平, 乔永旭, 喻景权, 等. 园艺植物果实糖积累的研究进展[J]. 中国农业科学, 2008, 41（4）:1151-1157.
	ZHANG Y P, QIAO Y X, YU J Q, et al. Progress of researches of sugar accumulation mechanism of horticultural plant fruits[J]. Scientia Agricultura Sinica, 2008, 41(4):1151-1157. DOI:10.3864/j.issn.0578-1752.2008.04.026.
23	KANG G Z, WANG Z X, SUN G C. Participation of H₂O₂ in enhancement of cold chilling by salicylic acid in banana seedlings[J]. Acta Botanica Sinica, 2003, 45(5):567-573.
24	DANG L J. Plants just say No to pathogens[J]. Nature, 1998, 394(6693):525-527. DOI:10.1038/28958.
25	HEINRICH M, HETTENHAUSEN C, LANGE T, et al. High levels of jasmonic acid antagonize the biosynthesis of gibberellins and inhibit the growth of Nicotiana attenuata stems[J]. The Plant Journal, 2013, 73(4): 591-606. DOI:10.1111/tpj.12058.
26	吕梦雯, 徐金光, 杜杰, 等. 外源赤霉素和多效唑对芍药冬季鳞芽发育的影响[J]. 植物生理学报, 2018, 54(5):790-802.
	LÜ M W, XU J G, DU J, et al. Effects of exogenous gibberellin A₃ and paclobutrazol on development of herbaceous peony (Paeonia lactiflora) bulbils[J]. Plant Physiology Journal, 2018, 54(5):790-802. DOI:10.13592/j.cnki.ppj.2017.0560.
27	VIDAL A M, BEN C W, MANUEL T, et al. Regulation of gibberellin 20⁃oxidase gene expression and gibberellin content in citrus by temperature and citrus exocortis viroid[J]. Planta, 2003, 217(3):442-448. DOI:10.1007/s00425-003-0999-2.
28	ZHANG Y Y, ZHANG B C, YAN D W, et al. Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation[J]. The Plant Journal: for Cell and Molecular biology,2011,67(2):342-353.DOI:10.1111/j.1365-313x.2011. 04596.x.
29	VARBANOVA M, YAMAGUCHI S, YANG Y, et al. Methylation of gibberellins by Arabidopsis GAMT1 and GAMT2[J]. The Plant Cell, 2007, 19(1):32-45. DOI:10.1105/tpc.106.044602.
30	SPONSEL V M. Gibberellins in Pisum sativum⁃their nature, distribution and involvement in growth and development of the plant[J]. 1985, 65(4):533-538. DOI:10.1111/j.1399-3054.1985.tb08686.x.
31	BINENBAUM J, WEINSTAIN R, SHANI E. Gibberellin localization and transport in plants[J]. Trends in Plant Science, 2018, 23: 410-421. DOI:10.1016/j.tplants.2018.02.005.
32	TAL I, ZHANG Y, JORGENSEN M E, et al. The Arabidopsis NPF3 protein is a GA transporter[J]. Nature Communications, 2016, 7:11486. DOI:10.1038/ncomms11486.
33	郑国生, 盖树鹏, 盖伟玲. 低温解除牡丹芽休眠进程中内源激素的变化[J]. 林业科学, 2009, 45(2): 48.
	ZHENG G S, GAI S P, GAI W L. Changes of endogenous hormones during dormancy release by chilling in tree peony[J]. Scientia Silvae Sinicae, 2009, 45(2): 48.
34	张佳平. 芍药在杭州栽培的耐热评价及地下芽休眠机理研究[D]. 杭州:浙江大学, 2015.
	ZHANG J P. Heat resistance evaluation and dormancy mechanism of the underground renewal bud of Paeonia lactiflora cultivated in Hangzhou City[D]. Hangzhou: Zhejiang University, 2015.
35	袁燕波, 王历慧, 于晓南. 芍药休眠芽发育进程内源激素变化研究[J]. 浙江农业学报, 2014, 26(1):54-60.
	YUAN Y B, WANG L H, YU X N. Comparative analysis of endogenous hormones during dormant bud development of Chinese herbaceous peony[J]. Acta Agriculturae Zhejiangensis, 2014, 26(1):54-60. DOI:10.3969/j.issn.1004-1524.2014.01.10.

环境 environment	GA₃处理水平/ （mg·L^-1） GA₃ concentration	发芽所需时长/d days to sprout	萌芽数 shoot number	地径/mm basal diameter	发芽到开花时长/d days from sprout to flower	折萎率/% wilting rates	株高/cm plant height	成花数 flower amount per pot	花径/cm flower diameter
室内 indoor	100	11	4.72±0.98 c	5.61±0.39 a	75	0.88	39.31±2.95 b	1.83±0.19 b	7.67±1.46 b
	200	10	6.85±1.05 b	5.22±0.65 a	72	21.25	41.86±1.63 b	1.56±0.21 b	8.12±0.68 b
	300	10	7.13±0.85 b	5.17±0.58 a	71	64.47	42.17±3.28 b	1.17±0.16 b	7.85±0.92 b
	1 000	8	8.84±1.37 a	4.45±0.98 b	—	100.00	15.45±1.87 c	—	—
室外 outdoor	0(CK)	151	5.12±1.45 c	6.20±0.86 a	51	0	51.38±0.86 a	3.43±0.33 a	9.21±0.79 a
	100	14	4.95±1.88 c	6.13±1.05 a	—	0.52	12.26±1.03 c	—	—
	200	13	6.92±1.23 b	5.34±0.72 a	—	18.12	15.45±1.24 c	—	—
	300	12	7.24±0.96 b	5.17±0.58 a	—	56.29	18.84±1.37 c	—	—
	1 000	10	8.56±1.24 a	4.56±0.74 b	—	100.00	14.28±0.72 c	—	—

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