<正>本试验采用聚丙烯酰胺凝胶垂直平板电泳,分析了黑杨派13个无性系。试验表明,美洲黑杨,欧洲黑杨及其杂种欧美杨的过氧化物酶同工酶酶谱图式有明显的差异。美洲黑杨具有3—4条谱带,Rf值为0.667的谱带是美洲黑杨特有的。加龙杨(Populus nigra cv.‘blanc de garonne’)是典型的欧洲黑杨,具有7条谱带,Rf值为0.533和0.613的谱带是欧洲黑杨特有的。欧美杨无性系具有4—8条谱带,其酶谱图式是不同的。高优势的欧美杨杂种兼有美洲黑杨和欧洲黑杨的谱带。 黑杨派各无性系的谱带数不同,其Rf值亦有差异。有的无性系酶谱相近似,Rf值相同,但在酶的活性上却有明显的不同。我们认为,用同工酶分析法对黑杨派无性系进行鉴定是可行的。

Electrophoreses on vertical polyacrylamide plate were adopted to analyse 13 clones of Aigioros poplars. Marked differences have been found in peroxide isozyme patterns in Populus nigra, P. deltoides and their hybrids Populus x Euramericana. Each clone of the P. deltoides has 3-4 bands of zymogram. The band with a Rf value of 0.667 is peculiar to P. deltoides. Populus nigra cv. "blane de garonne," a typical clone of P. nigra, has 7 bands of zymogram. Bands with Rf values 0.535 and 0.613 are peculiar to "blane de garonne." Clones of Populus x Euramericand have 4-g bands. They are varied in isozyme patterns. The highly vigorous hybrids of Populus x Euramericana have both the same bands as P. nigra and P. deltoides.The Aigioros clones not only vary in band number but also differ in band Rf value. Some clones are generally alike in their zymograms and similar in Rf values, but vary in enzyme activities.Consequently, we hold that the method of isozyme analysis for identifying Aigioros clones is feasible,- 777 -