Abstract: Mycelia of Trichoderma reesei Rut C30 were adsorbed and immobilized effectively on pieces of porous plastics. In repeated batch fermentations using sulphate pulp as the substrate of cellulase production, the filter paper and cellobiase activity produced by the immobilized cells were 2. 4 IU·mL-1 and 0. 2 IU·mL-1 respectively, which were 1. 6 times and 2. 2 times as high as that of the free cell fermentation separately. The immobilized cells were stable after having been continuously used for more than 40 days. The results of enzymatic hydrolysis have shown that the hydrolytic ability of the enzyme which was produced by the immobilized cells was very effective to lignocellulosic materials, when the enzyme dosage per gram substrate was above 20 IU, the efficiency of enzymatic hydrolysis was over 90 percent.