Abstract: By Gray Relation Analysis, soil fertility was evaluated according to the biochemical variation of the microbes quantities, the nutrient contents and the enzyme activities in six types of forestry litters and soils. The conclusion was made as follows: Oak soil>Pine mixed Oak soil > Bamboo mixed Oak soil > Bamboo soil>Pine soil >Chinese fir soil warranted by their relation grades as 0. 841, 0. 813, 0. 786, 0. 773, 0. 690,0. 685. These results were consistent to the researches of Xiashu Forestry Ecological Station and indicated that this method was suitable for the apprehensive evaluation for the soil fertility. In general, this study gives some novel experiences and methods for further researches on forestry soil fertility.