Abstract: Citric acid esters of partial palmitic acid glycerides are a kind of system of many components with very complex polarisabilities,so that it is difficult to separate them from each other.The mixtures can not be separated effectively by solvent systems of n hexane ether formic acid(80∶20∶2) recommenced in references.In this paper,silica G+1% carboxymethyl cellulose(CMC) + 0.6 mol/L boric acid were used as the thin layer chromatography condition,and the solvent system for thin layer chromatography was optimized using the uniform design method,the optimal system for Citric acid esters of partial palmitic acid glycerides was trichloromethane proanone acetic acid (85∶15∶1),the results accorded with one of electron spray ionization mass spectrum(ESI-MS).