<正>采用 RAPD技术分析了 1 6个球孢白僵菌菌株的遗传分化。由 1 6 0个随机引物中筛选出 2 7个引物对各菌株进行 PCR扩增。结果表明从 1 6个球孢白僵菌菌株中共获 3 0 1个 RAPD标记 ,其中多态性标记 2 88个 ,占 95.7% ,表明菌株间具有较丰富的遗传多态性。聚类分析把1 6个球孢白僵菌菌株分为三大类群 (1 ) Blh、Bfj、Bgd和 Bpy;(2 ) By7、F- 2 6 3、RECBb0 1、B12 和 B13;(3 ) B7、B11、Bxs、B6 、By2 、Bpc和 Bzs。菌株 DNA多态性与采集地之间有一定的相关性 ,但与寄主来源、孢子形态和对松墨天牛幼虫的毒力间未表现出明显的相关性

primers were selected from 160 primers for RAPD analysis of 16 strains of B. bassiana .Altogether 301 scorable DNA fragments were recorded. Among them, 288 were polymorphic,and accounted for 95.7%.A dendrogram generated by UPGMA method would divide 16 strains into three distinct groups, (1) B lh , B fj , B gd and B py ; (2) B y7 , F 263, RECBb01, B 12 and B 13 ; and (3)B 7,B 11 , B xs , B 6 , B y2 , B pc and B zs . The cluster results were certainly associated with geographic regions, rather than original hosts, conidiospore size and virulence on Monochamus alternatus larvae.