Abstract: primers were selected from 160 primers for RAPD analysis of 16 strains of B. bassiana .Altogether 301 scorable DNA fragments were recorded. Among them, 288 were polymorphic,and accounted for 95.7%.A dendrogram generated by UPGMA method would divide 16 strains into three distinct groups, (1) B lh , B fj , B gd and B py ; (2) B y7 , F 263, RECBb01, B 12 and B 13 ; and (3)B 7,B 11 , B xs , B 6 , B y2 , B pc and B zs . The cluster results were certainly associated with geographic regions, rather than original hosts, conidiospore size and virulence on Monochamus alternatus larvae.