Abstract: The chromosomes of Pinus Massoniana collecte d from the 7 provenances in the south of China were analyzed on their fluorecent banding pattern by squotial staining with the two kinds of specificially bandin g fluorochroums,CMA and DAPI .Results were as follows:(1)There were five types of the CMA-banding in the chromosomes of Pinus massoniana. It had a pair o f metacentric chromosome with two CMA-bands at both their proximal and intersti tial regions (type A), six pairs of metacentric chromosome with interstitial CMA -band(type B),a pair of metacentric chromosome with the proximal CMA-band (typ e C),and three pairs of metercentric or submetacentric chromosome without CMA-b and (type D) as well as a pair of submetercentric chromosome with proximal CMA- band (type E).Its CMA band formula was 2n=24=2A+12B+2C+6D+2E.(2)The seven prov enances of P.massoniana were calssified into two groups by CMA-banding patt ern.(3) The DAPI banding pattern of the seven provenances of P.massoniana was compared. The numbers of fluorescence banding of the provenance in southern region were more than those of the provenance in middle region.The numbers of fl uorescence banding of provenance in the western region were more than those of p rovenance in eastern region.