摘要
<正>利用ＣＴＡＢ方法提取银杏、板栗的冬芽、嫩叶、半木质化韧皮部、老枝韧皮部和成熟叶子的 ＤＮＡ。结果表明，除成熟叶子外，其它 ４种组织的 ＤＮＡ 较容易提取，且 ＤＮＡ的纯度较高，Ａ２６０／Ａ２８０值都在 １．８以上，每 １００ ｍｇ材料的产量为 ２２～５０Ｇμｇ。清晰明亮的 ＰＣＲ产物也表明所提取的ＤＮＡ可满足ＤＮＡ分子标记的要求。
Abstract
This paper shows that the genomic DNA from different tissues of Ginkgo and Chinese chest- nut was extracted by CTAB method. The results indicate that except for mature leaf, other tissues, uch as dormant bud, young leaf, phloem and half-lignous phloem, can be used to extract DNA easily and successfully. In addition,the value of A260/A280 for each DNA samples above mentioned is over 1. 8,and the quantity is over 22~50 g per 100 mg. Also,clear and bright products of RAPD reveal that the DNA may meet needs of DNA molecular markers.
沈永宝;施季森.
银杏、板栗不同组织DNA的提取[J]. 南京林业大学学报（自然科学版）. 2001, 25(06): 80-82 https://doi.org/10.3969/j.jssn.1000-2006.2001.06.021
SHEN Yong-bao.SHI Ji-sen.
DNA Extraction from Different Tissues of Ginkgo and Chinese Chestnut[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2001, 25(06): 80-82 https://doi.org/10.3969/j.jssn.1000-2006.2001.06.021
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