Abstract: The importance of indirectuse value of biodiversity of nature reserves is becoming more and more recognized.Due to the divergence in valuation theory and methods,the results of the studies are not accepted in practices.This paper evaluates the indirectuse value of biodiversity of Yaoluoping nature reserve by using alternative cost methods,opportunity cost methods and shadow prices.Using such an approach,the indirectuse value of biodiversity of the reserve is estimated at 255 million RMB,being a part of the total economic value of biodiversity in the reserve.The ecological functions assessed are relate to six aspects:soil conservation,water conservation,gas regulation,nutrient cycling,pollutant absorption and pest control.The result is adjusted according to the regional development phase coefficient and current value is 71 million RMB on the basis of 2000.Our work will contribute to the conservation of the nature reserves and provid an example of the economic evaluation of indirectuse value of biodiversity of nature reserves by offering an assessing valuing indicator system,pricing parameter and measuring model.